A5074719167- Muscle Physiology and Disorders
- PARP inhibition in cancer therapy
- DNA Repair Mechanisms
- Immunotherapy and Immune Responses
- Agriculture and Rural Development Research
- Animal Behavior and Welfare Studies
- Metal complexes synthesis and properties
- Health, Medicine and Society
- Folate and B Vitamins Research
- Cell death mechanisms and regulation
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Human-Animal Interaction Studies
- vaccines and immunoinformatics approaches
- Autophagy in Disease and Therapy
- CAR-T cell therapy research
- Microtubule and mitosis dynamics
- Endoplasmic Reticulum Stress and Disease
- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- Mitochondrial Function and Pathology
- bioluminescence and chemiluminescence research
- Obesity and Health Practices
- Extracellular vesicles in disease
- Amino Acid Enzymes and Metabolism
Inserm
2013-2024
Centre National de la Recherche Scientifique
2013-2024
Nestlé (Switzerland)
2024
Université Claude Bernard Lyon 1
2023-2024
Institut NeuroMyoGène
2023-2024
Hôpital de la Timone
2024
L'Institut Agro
2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Université Laval
2019-2020
Université Paris Cité
2013-2018
Abstract Non–small cell lung carcinoma patients are frequently treated with cisplatin (CDDP), most often yielding temporary clinical responses. Here, we show that PARP1 is highly expressed and constitutively hyperactivated in a majority of human CDDP-resistant cancer cells distinct histologic origin. Cells manifesting elevated intracellular levels poly(ADP-ribosyl)ated proteins (PARhigh) responded to pharmacologic PARP inhibitors as well PARP1-targeting siRNAs by initiating DNA damage...
The foundation of modern vaccinology dates back to the 1790s, when English physician Edward Jenner uncovered tremendous medical potential prophylactic vaccination. Jenner's work ignited a wave nationwide vaccination campaigns abating incidence multiple life-threatening infectious diseases and culminating with eradication natural smallpox virus, which was definitively certified by WHO in 1980. possibility using vaccines against cancer first proposed at end 19th century Paul Ehrlich William...
The antineoplastic agent cis-diammineplatinum(II) dichloride (cisplatin, CDDP) is part of the poorly effective standard treatment non-small cell lung carcinoma (NSCLC). Here, we report a novel strategy to improve efficacy CDDP. In conditions in which CDDP alone or either two PARP inhibitors, PJ34 hydrochloride hydrate CEP 8983, used as standalone treatments were inefficient killing NSCLC cells, combination plus that 8983 found kill substantial fraction cells. This cytotoxic synergy could be...
Significance One hypothetical pathway leading to oncogenic transformation involves a transient phase of tetraploidization, followed by asymmetric cell divisions, aneuploidy, and genomic instability. By means pharmacological screen, we identified resveratrol salicylate as compounds that kill tetraploid cells more efficiently than their parental diploid counterparts. Resveratrol reduced the frequency arising from primary epithelial cultures exposed mitotic inhibitors. In mouse model intestinal...
Skeletal muscle relies on resident stem cells (MuSCs) for growth and repair. Aging diseases impair MuSC function, leading to cell exhaustion regenerative decline that contribute the progressive loss of skeletal mass strength. In absence clinically available nutritional solutions specifically targeting MuSCs, we used a human myogenic progenitor (hMP) high-content imaging screen natural molecules from food identify nicotinamide (NAM) pyridoxine (PN) as bioactive nutrients stimulate MuSCs have...
Vitamin B6 metabolism influences the adaptive response of non-small lung carcinoma (NSCLC) cells to distinct, potentially lethal perturbations in homeostasis, encompassing nutrient deprivation, hyperthermia, hypoxia, irradiation as well exposure cytotoxic chemicals, including DNA-damaging agent cisplatin (CDDP). Thus, siRNA-mediated downregulation pyridoxal kinase (PDXK), enzyme that generates bioactive form vitamin B6, protects NSCLC (as a large collection human and murine malignant...
The immune system avoids oncogenesis and slows down tumor progression through a mechanism called immunosurveillance. Nevertheless, some malignant cells manage to escape from control form clinically detectable tumors. Tetraploidy, which consists in the intrinsically unstable duplication of genome, is considered as (pre)-cancerous event that can result aneuploidy contribute oncogenesis. We previously described fact tetraploid be eliminated by system. Here, we investigate role different innate...
The balance of phospho-signaling at the outer kinetochore is critical for forming accurate attachments between kinetochores and mitotic spindle timely exit from mitosis. A major player in determining this PP2A-B56 phosphatase, which recruited to kinase attachment regulatory domain (KARD) budding uninhibited by benzimidazole 1-related 1 (BUBR1) a phospho-dependent manner. This unleashes rapid, switch-like phosphatase relay that reverses phosphorylation kinetochore, extinguishing checkpoint...
Accelerometer-based mobility scoring has focused on cow behaviors such as lying and walking. Accuracy levels high 91% have been previously reported. However, there limited replication of results. Here, measures identified indicative mobility, bouts walking time, were examined. On a research farm commercial farm, 63 grazing cows’ behavior was monitored in four trials (16, 16, 15 cows) using leg-worn accelerometers. Seventeen good (score 0), 23 imperfect 1), 22 mildly impaired 2) cows...
Abstract Skeletal muscle regeneration is mediated by resident stem cells that produce progeny to repair or recreate muscle. Critical this function the ability transition between states of proliferation and quiescence. This balancing act disrupted with age, leading eroded regenerative capacity. Notwistanding, mechanisms which regenerating niche directs MuSCs return dormant state are largely unknown. Since single-cell RNAseq methods exclude analysis multinucleated cells, we generated...
ABSTRACT Modulations in chromatin structure orchestrate gene expression and direct stem cell fate. More specifically, the histone 3 lysine 9 Methyltransferase Setdb1 controls transcriptional repression to regulate pluripotency self-renewal. While functions have been extensively studied embryonic cells cancer cells, less is known on its role adult vivo . Here, we show that by muscle (MuSCs) required for tissue regeneration following acute injury. We find SETDB1 represses of endogenous...