A5084208566- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Ion Transport and Channel Regulation
- Blood properties and coagulation
- Neuroscience and Neuropharmacology Research
- Neonatal Health and Biochemistry
- Cardiac electrophysiology and arrhythmias
- Pancreatic function and diabetes
- Parathyroid Disorders and Treatments
- Ion Channels and Receptors
- Calcium signaling and nucleotide metabolism
- Magnesium in Health and Disease
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Electrolyte and hormonal disorders
- Hormonal Regulation and Hypertension
- Cell death mechanisms and regulation
- Metabolism, Diabetes, and Cancer
- Protein Kinase Regulation and GTPase Signaling
- Lipid Membrane Structure and Behavior
- Biomedical Research and Pathophysiology
- Sphingolipid Metabolism and Signaling
- Receptor Mechanisms and Signaling
- Renal function and acid-base balance
- Diet and metabolism studies
University of Duisburg-Essen
2013-2025
Hangzhou Wanxiang Polytechnic
2025
Zhejiang University
2025
University of Tübingen
2015-2024
Siemens (Germany)
2013-2024
Max Planck Institute for Extraterrestrial Physics
2023-2024
École Polytechnique Fédérale de Lausanne
2022
University Children's Hospital Tübingen
1997-2021
Réseau Fribourgeois de Santé Mentale
2017-2021
Heinrich Heine University Düsseldorf
1996-2020
Background/Aims: Human parvovirus B19 (B19V) may cause inflammatory cardiomyopathy (iCMP) which is accompanied by endothelial dysfunction. The B19V capsid protein VP1 contains a lysophosphatidylcholine producing phospholipase A2 (PLA) sequence. Lysophosphatidylcholine has in turn been shown to inhibit Na+/K+ ATPase. present study explored whether modifies ATPase activity. Methods:Xenopus oocytes were injected with cRNA encoding isolated from patient suffering fatal B19V-iCMP or PLA2-negative...
Lang, Florian, Gillian L. Busch, Markus Ritter, Harald Völkl, Siegfried Waldegger, Erich Gulbins, and Dieter Häussinger. Functional Significance of Cell Volume Regulatory Mechanisms. Physiol. Rev. 78: 247–306, 1998. — To survive, cells have to avoid excessive alterations cell volume that jeopardize structural integrity constancy intracellular milieu. The function cellular proteins seems specifically sensitive dilution concentration, determining the extent macromolecular crowding. Even at...
Until now, a lack of inhibitors with high potency and selectivity in vivo has hampered investigation the p38 mitogen-activated protein kinase (MAPK) signaling pathway. We describe design skepinone-L, which is, to our knowledge, first ATP-competitive MAPK inhibitor excellent efficacy selectivity. Therefore, skepinone-L is valuable probe for chemical biology research, it may foster development unique class inhibitors.
Background/Aims: The serum- and glucocorticoid-inducible kinase Sgk1 contributes to cardiac remodeling development of heart failure, which is paralelled by Sgk1-dependent stimulation the Na+/H+ exchanger Nhe1. Glucocorticoids are powerful stimulators expression influence remodeling. present study thus explored whether glucocorticoid receptor agonist dexamethasone influenced expression, as well activity, phosphorylation at Ser703 Methods: Experiments were performed in HL-1 cardiomyocytes gene...
The serum- and glucocorticoid-inducible kinase-1 (SGK1) is ubiquitously expressed under genomic control by cell stress (including shrinkage) hormones gluco- mineralocorticoids). Similar to its isoforms SGK2 SGK3, SGK1 activated insulin growth factors via phosphatidylinositol 3-kinase the 3-phosphoinositide-dependent kinase PDK1. SGKs activate ion channels (e.g., ENaC, TRPV5, ROMK, Kv1.3, KCNE1/KCNQ1, GluR1, GluR6), carriers NHE3, GLUT1, SGLT1, EAAT1–5), Na + -K -ATPase. They regulate...
Previously we cloned a polyspecific transporter from rat (rOCT1) that is expressed in renal proximal tubules and hepatocytes mediates electrogenic uptake of organic cations with different molecular structures. Recently homologous kidney (rOCT2) was but not characterized detail. We report cloning characterization two transporters man (hOCT1 hOCT2) displaying approximately 80% amino acid identity to rOCT1 rOCT2, respectively. Northern blots showed hOCT1 mainly transcribed liver, while hOCT2...
To clarify the physiological role of Na(+)-D-glucose cotransporter SGLT1 in small intestine and kidney, Sglt1(-/-) mice were generated characterized phenotypically. After gavage d-glucose, intestinal glucose absorption across brush-border membrane (BBM) via GLUT2 analyzed. Glucose-induced secretion insulinotropic hormone (GIP) glucagon-like peptide 1 (GLP-1) wild-type compared. The impact on renal handling was investigated by micropuncture studies. It observed that developed a...
Transport of lactate and other monocarboxylates in mammalian cells is mediated by a family transporters, designated monocarboxylate transporters (MCTs). The MCT4 member this has recently been identified as the major isoform white muscle cells, mediating efflux out glycolytically active myocytes [Wilson, Jackson, Heddle, Price, Pilegaard, Juel, Bonen, Montgomery, Hutter Halestrap (1998) J. Biol. Chem. 273, 15920–15926]. To analyse functional properties transporter, rat was expressed Xenopus...
AbstractSurvival of human and animal cells requires avoidance excessive alterations cell volume. The osmolarity amassed by cellular accumulation organic substances must be compensated lowering cytosolic ion concentrations. Na+/K+ ATPase extrudes Na+ in exchange for K+, which can permeate the membrane through K+ channels. exit generates a cell-negative potential difference across membrane, driving anions such as Cl−. low Cl− concentrations counterbalance excess substances. Cell volume...
Exposure of erythrocytes to the Ca2+ ionophore ionomycin has recently been shown induce cell shrinkage, membrane blebbing, and breakdown phosphatidylserine asymmetry, all features typical apoptosis nucleated cells. Although asymmetry is thought result from activation a Ca2+-sensitive scramblase, mechanism role shrinkage have not explored. The present study was performed test whether ionomycin-induced Gardos K+ channels subsequent participate in human erythrocytes. According on-cell...
Paroxysmal dyskinesias are episodic movement disorders that can be inherited or sporadic in nature. The pathophysiology underlying these remains largely unknown but may involve disrupted ion homeostasis due to defects cell-surface channels nutrient transporters. In this study, we describe a family with paroxysmal exertion-induced dyskinesia (PED) over 3 generations. Their PED was accompanied by epilepsy, mild developmental delay, reduced CSF glucose levels, hemolytic anemia echinocytosis,...
A high intake of dietary salt (NaCl) has been implicated in the development hypertension, chronic inflammation, and autoimmune diseases. We have recently shown that a proinflammatory effect boosts activation Th17 cells classical, LPS-induced macrophages (M1). Here, we examined how alternative (M2) is affected by salt. In stark contrast to M1 macrophages, blunted BM-derived mouse stimulated with IL-4 IL-13, M(IL-4+IL-13) macrophages. Salt-induced reduction was not associated increased...
Hepatic metabolism and gene expression are among other regulatory mechanisms controlled by the cellular hydration state, which changes rapidly in response to anisotonicity, concentrative substrate uptake, oxidative stress, under influence of hormones such as insulin glucagon. Differential screening for cell volume sensitive transcripts a human hepatoma line revealed putative serine/threonine kinase, h-sgk, has 98% sequence identity serum- glucocorticoid regulated sgk, cloned from rat mammary...