A5082143634- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Spaceflight effects on biology
- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Microfluidic and Bio-sensing Technologies
- Wnt/β-catenin signaling in development and cancer
- Cellular transport and secretion
- Force Microscopy Techniques and Applications
- Kruppel-like factors research
- Axon Guidance and Neuronal Signaling
- Micro and Nano Robotics
- Glycosylation and Glycoproteins Research
- Connective tissue disorders research
- S100 Proteins and Annexins
- Connexins and lens biology
- Cancer Cells and Metastasis
- Heat shock proteins research
- Nuclear Structure and Function
- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- Erythrocyte Function and Pathophysiology
Centre National de la Recherche Scientifique
2012-2023
Institut de Biologie du Développement Marseille
2007-2023
Aix-Marseille Université
2014-2021
Institute for Bioengineering of Catalonia
2012-2019
Barcelona Institute for Science and Technology
2018
Universitat de Barcelona
2016
Collective cell migration (CCM) is essential for organism development, wound healing, and metastatic transition, the primary cause of cancer-related death, it involves cell–cell adhesion molecules cadherin family. Increased P-cadherin expression levels are correlated with tumor aggressiveness in carcinoma aggressive sarcoma; however, how promotes malignancy remains unknown. Here, using integrated biology biophysical approaches, we determined that specifically induces polarization CCM through...
Collective cell migration in spreading epithelia controlled environments has become a landmark our current understanding of fundamental biophysical processes development, regeneration, wound healing or cancer. Epithelial monolayers are treated as thin layers viscous fluid that exert active traction forces on the substrate. The model is exactly solvable and shows broad range applicabilities for quantitative analysis interpretation force microscopy data from variety experiments lines. In...
A general trait of cell monolayers is their ability to exert contractile stresses on surroundings. The scaling laws that link such with the size and geometry constituent cells remain largely unknown. In this Letter, we show active tension an epithelial monolayer scales linearly cells, a surprisingly simple relationship. slope relationship defines tensile modulus, which depends concentration myosin spans more than 2 orders magnitude across types molecular perturbations.
Although columnar epithelial cells are known to acquire an elongated shape, the mechanisms involved in this morphological feature have not yet been completely elucidated. Using human intestinal Caco2 cells, it was established here that levels of drebrin E, actin-binding protein, increase terminal web both vitro and vivo during formation apical domain. Drebrin E depletion found impair cell compaction elongation processes monolayer without affecting polarity or tight junctions. Decreasing...
The Crumbs (Crb) complex is a key epithelial determinant. To understand its role in morphogenesis, we examined function the Drosophila pupal wing, an epithelium undergoing hexagonal packing and formation of planar-oriented hairs. Crb distribution dynamic, being stabilized to subapical region just before hair formation. Lack crb or stardust, but not DPatj, affects delays formation, without impairing polarities with increased fluctuations cell junctions perimeter length, fragmentation adherens...
Fundamental processes in cell adhesion, motility, and rigidity adaptation are regulated by integrin-mediated adhesion to the extracellular matrix (ECM). The link between ECM component fibronectin (fn) integrin α5β1 forms a complex with ZO-1 cells at edge of migrating monolayers, regulating migration. However, how this affects α5β1-fn is unknown. Here we show that α5β1/ZO-1 decreases resistance force adhesions located monolayers while also increasing recruitment. Consistently molecular clutch...
Directional collective cell migration (DCCM) is crucial for morphogenesis and cancer metastasis. P-cadherin (also known as CDH3), which a cell-cell adhesion protein expressed in carcinoma aggressive sarcoma cells associated with poor prognosis, major DCCM regulator. However, it unclear how P-cadherin-mediated mechanical coupling between migrating influences force transmission to the extracellular matrix (ECM). Here, we found that decorin, small proteoglycan binds organizes collagen fibers,...
Ciliated epithelia perform essential functions in animals across evolution, ranging from locomotion of marine organisms to mucociliary clearance airways mammals. These are composed multiciliated cells (MCCs) harboring myriads motile cilia, which rest on modified centrioles called basal bodies (BBs), and beat coordinately generate directed fluid flows. Thus, BB biogenesis organization is central MCC function. In eukaryotes, the coiled-coil domain proteins Lrrcc1 Ccdc61 have previously been...
Abstract Polarity protein complexes function during polarized cell migration and a subset of these proteins localizes to the reoriented centrosome this process. Despite observations, mechanisms behind recruitment polarity such as aPKC/PAR6α complex are not well understood. Here we identify Hook2 an interactor for that functions localize at centrosome. We first demonstrate is essential Golgi re-orientation towards front. Depletion results in decrease PAR6α migration, while overexpression...
Intestinal epithelial cells are highly polarized and exhibit a complex architecture with columnar shape specialized apical surface supporting microvilli organized in brush border. These rooted dense meshwork of acto-myosin called the terminal web. We have shown recently that Drebrin E, an F-actin-binding protein, is key protein for organization web E also required cell Caco2 (human colonic cells). Here, we found subcellular localization several markers including dipeptidyl peptidase IV...
Abstract Ciliated epithelia perform a variety of essential functions across animal evolution, ranging from locomotion marine organisms to mucociliary clearance airways in mammals. These are composed multiciliated cells (MCCs) harbouring myriads motile cilia, which rest on modified centrioles called basal bodies (BBs), and beat coordinately generate directed fluid flows. Thus, BB biogenesis organization is central MCC function. In eukaryotes, the coiled-coil domain proteins Lrrcc1 Ccdc61 were...
Summary Several cellular processes during morphogenesis, tissue healing or cancer progression involve epithelial to mesenchymal plasticity that leads collective motion (plasticity?). Even though a rich variety of EMP programs exist, major hallmark unifying them is the initial breaking symmetry modifies phenotype and axis polarity. During this process, actin cytoskeleton junctions are extensively remodelled correlating with build-up mechanical forces. As migration proceeds, forces generated...