A5071004929- Cell Adhesion Molecules Research
- Spectroscopy and Laser Applications
- Nuclear Materials and Properties
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Advanced Chemical Physics Studies
- Amino Acid Enzymes and Metabolism
- TGF-β signaling in diseases
- Molecular Spectroscopy and Structure
- Atmospheric Ozone and Climate
- Combustion and Detonation Processes
- Nuclear reactor physics and engineering
- Laser Design and Applications
- S100 Proteins and Annexins
- Particle Dynamics in Fluid Flows
- Aerosol Filtration and Electrostatic Precipitation
- HER2/EGFR in Cancer Research
- Risk and Safety Analysis
- Cancer Cells and Metastasis
- Nuclear Engineering Thermal-Hydraulics
- Nuclear and radioactivity studies
- Cancer Immunotherapy and Biomarkers
- Land Use and Ecosystem Services
- Atomic and Subatomic Physics Research
- Cellular Mechanics and Interactions
Queen Mary University of London
2010-2025
Cancer Research UK
2005-2023
Ecological Society of America
2016-2019
University of Tennessee at Chattanooga
2019
University of North Dakota
2018
Barts Health NHS Trust
2013-2016
University of East Anglia
2013
Southampton General Hospital
2013
AGH University of Krakow
2013
Southern Taiwan University of Science and Technology
2013
A slip correction factor is used to correct Stokes' law for the fact that no-slip boundary condition violated small aerosol particles moving with respect gaseous medium. The Knudsen-Weber form of given by C(Kn)= 1 + Kn[α β exp(—γ/Kn)]. parameters α, β, and γ are customarily those based upon experiments reported in 1917 1923 R. A. Millikan droplets oil. Because differences molecular interactions surfaces solid oil drops, different should be appropriate particles. In this study an improved...
Abstract For photoheterotrophic growth, a Chlamydomonas reinhardtii cell requires at least 1.7 × 107 manganese ions in the medium. At lower ion concentrations (typically <0.5 μ m), cells divide more slowly, accumulate less chlorophyll, and culture reaches stationary phase density. Below 0.1 m supplemental medium, are photosynthetically defective. This is accompanied by decreased abundance of D1, which binds Mn4Ca cluster, release OEE proteins from membrane. Assay Mn superoxide...
Targeted therapies have yet to significant impact on the survival of patients with bladder cancer. In this study, we focused urea cycle enzyme argininosuccinate synthetase 1 (ASS1) as a therapeutic target in cancer, based our discovery prognostic and functional import ASS1 setting. expression status tumors from 183 Caucasian 295 Asian was analyzed, along its hypothesized association clinicopathologic features, including tumor size invasion. Furthermore, genetics, biology, implications loss...
Aberrant promoter DNA hypermethylation is a hallmark of cancer; however, whether this sufficient to drive cellular transformation not clear. To investigate question, we use CRISPR-dCas9 epigenetic editing tool, where an inactive form Cas9 fused methyltransferase effectors. Using system, here show simultaneous de novo methylation genes commonly methylated in cancer, CDKN2A, RASSF1, HIC1 and PTEN primary breast cells isolated from healthy human tissue. We find that maintained even the absence...
ABSTRACT Previously, we had identified FOX1 and FTR1 as iron deficiency-inducible components of a high-affinity copper-dependent uptake pathway in Chlamydomonas . In this work, survey the version 3.0 draft genome to identify ferrireductase, FRE1, two ZIP family proteins, IRT1 IRT2, candidate ferrous transporters based on their increased expression iron-deficient versus iron-replete cells. parallel proteomic approach, FEA1 FEA2 major proteins secreted by reinhardtii The recovery from medium...
Abstract Introduction The stromal microenvironment has a profound influence on tumour cell behaviour. In tumours, the extracellular matrix (ECM) composition differs from normal tissue and allows novel interactions to function. ECM protein tenascin-C (TNC) is frequently up-regulated in breast cancer we have previously identified two isoforms – one containing exon 16 (TNC-16) exons 14 plus (TNC-14/16). Methods present study analysed functional significance of this altered TNC isoform profile...
Fe deficiency is one of several abiotic stresses that impacts plant metabolism because the loss function Fe-containing enzymes in chloroplasts and mitochondria, including cytochromes, FeS proteins, superoxide dismutase (FeSOD). Two pathways increase capacity Chlamydomonas reinhardtii chloroplast to detoxify during limitation stress. In pathway, MSD3 upregulated at transcriptional level up 103-fold response limitation, leading synthesis a previously undiscovered plastid-specific MnSOD whose...
This study investigated the functional and clinical significance of integrin αvβ6 upregulation in myoepithelial cells ductal carcinoma situ (DCIS).Archival samples DCIS with associated invasion (n = 532) were analyzed for expression by immunohistochemistry ability to predict recurrence progression assessed an independent, unique cohort cases long-term follow-up. Primary cell lines, without expression, used measure effect on growth tumor lines vitro a xenograft mouse model. Involvement TGFβ...
cAMP-dependent protein kinase A (PKA) is important in processes requiring localized cell protrusion, such as migration and axonal path finding. Here, we used a membrane-targeted PKA biosensor to reveal activation of at the leading edge migrating cells. Previous studies show that activity promotes protrusion efficient migration. In live cells, membrane-associated was highest required ligation integrins alpha4beta1 or alpha5beta1 an intact actin cytoskeleton. alpha4 are type I PKA-specific...
The frequent expression of latent membrane proteins LMP2A and LMP2B in Epstein Barr virus (EBV)-associated tumors suggests that these play a role EBV-induced epithelial cell growth transformation. Expression had no effect on the morphology squamous cells monolayer culture, but their was associated with an increased capacity to spread migrate extracellular matrix. Although mechanisms by which promote spreading motility are unclear, use selective pharmacological inhibitors has established for...
Abstract Two unlinked genes FER1 and FER2 encoding ferritin subunits were identified in the Chlamydomonas genome. An improved gene model, built on basis of manual sequencing incorporation unplaced reads, indicated 49% identity between subunits. Both transcripts are increased abundance as iron nutrition is decreased but pattern for each distinct. Using subunit-specific antibodies, we monitored expression at protein level. In response to low iron, ferritin1 complex parallel increase mRNA....
Proteases have long been associated with cancer progression, due to their ability facilitate invasion upon matrix remodelling. However, proteases are not simply degraders of the matrix, but also play fundamental roles in modulating cellular behaviour through proteolytic processing specific substrates. Indeed, can elicit both pro- and anti- tumorigenic effects depending on context. Using a heterocellular spheroid model breast we demonstrate repressive function myoepithelial ADAMTS3, its loss...
Abstract Ductal carcinoma in situ (DCIS) is a non-obligate precursor of invasive breast cancer. Virtually all women with DCIS are treated, despite evidence suggesting up to half would remain stable, non-threatening, disease. Overtreatment thus presents pressing issue management. To understand the role normally tumour suppressive myoepithelial cell disease progression we present 3D vitro model incorporating both luminal and cells physiomimetic conditions. We demonstrate that DCIS-associated...
The drag force on aggregate particles of uniform spheres was measured in a Millikan apparatus as function Knudsen number. Our experiment designed to study the effect particle orientation slip correction factor nonspherical particles. velocities charged gravitational field with and without an applied electrical were measured. An strength 2000 V/cm used align doublet triplet Results showed that moved random while field. same its polar axis parallel electric (doublets) or plane centers...
Real-time tracking of kinase activity in living systems has revealed new modes encoding signaling information into spatiotemporal patterns and opened avenues for screening modulators. However, the sensitivity detection, which is commonly coupled to a fluorescence resonance energy transfer (FRET)-based readout, often been limiting factor. Here we show that kinase-inducible bimolecular switch consisting substrate interest phosphoamino acid binding domain can be designed sense different...
Normal myoepithelial cells (MECs) play an important tumour-suppressor role in the breast but display altered phenotype ductal carcinoma situ (DCIS), gaining tumour-promoter functions. Matrix metalloproteinase-8 (MMP-8) is expressed by normal MECs lost DCIS. This study investigated function of MMP-8 and impact its loss Primary DCIS-associated MECs, (N-1089) DCIS-modified (β6-1089) cell lines, were used to assess expression function. β6-1089 lacking transfected with WT catalytically inactive...