A5019096353- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Advanced Proteomics Techniques and Applications
- Protein Structure and Dynamics
- Heat shock proteins research
- ATP Synthase and ATPases Research
- Lipid metabolism and biosynthesis
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Mass Spectrometry Techniques and Applications
- Plant nutrient uptake and metabolism
- Microbial Metabolic Engineering and Bioproduction
- Microtubule and mitosis dynamics
- Light effects on plants
- Fungal Plant Pathogen Control
- Plant Stress Responses and Tolerance
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Parkinson's Disease Mechanisms and Treatments
- Nuclear Receptors and Signaling
- Plant Disease Resistance and Genetics
- Spectroscopy and Quantum Chemical Studies
- Child Nutrition and Feeding Issues
University of Kaiserslautern
2015-2024
Rheinland-Pfälzische Technische Universität Kaiserslautern-Landau
2023-2024
University of Koblenz and Landau
2023
Chinese Academy of Sciences
2019
Max Planck Institute of Molecular Plant Physiology
2009-2018
Max Planck Society
2011
University of California, Los Angeles
2008-2010
University of Freiburg
2002-2008
Windward Community College
2008
University of Pennsylvania
2005-2006
Nitrogen (N) is a key nutrient that limits global primary productivity; hence, N-use efficiency of compelling interest in agriculture and aquaculture. We used Chlamydomonas reinhardtii as reference organism for multicomponent analysis the N starvation response. In presence acetate, respiratory metabolism prioritized over photosynthesis; consequently, N-sparing response targets proteins, pigments, RNAs involved photosynthesis chloroplast function those respiration. Transcripts proteins...
Significance Eukaryotic algae, which play a fundamental role in global CO 2 fixation, enhance the performance of carbon-fixing enzyme Rubisco by placing it into an organelle called pyrenoid. Despite ubiquitous presence and biogeochemical importance this organelle, how assembles to form pyrenoid remains long-standing mystery. Our discovery abundant repeat protein that binds represents critical advance our understanding biogenesis. The sequence suggests elegant models explain structural...
Botrytis cinerea is a major plant pathogen infecting more than 1400 species. During invasion, the fungus rapidly kills host cells, which believed to be supported by induction of programmed cell death. To comprehensively evaluate contributions most currently known death inducing proteins (CDIPs) and metabolites for necrotrophic infection, an optimized CRISPR/Cas9 protocol was established allowed perform serial marker-free mutagenesis generate multiple deletion mutants lacking up 12 CDIPs....
We investigated the systems response of metabolism and growth after an increase in irradiance nonsaturating range algal model Chlamydomonas reinhardtii. In a three-step process, photosynthesis levels metabolites increased immediately, 10 to 15 min, transcript protein abundance responded by 40 120 240 respectively. first phase, starch provided transient buffer for carbon until increased. This uncouples from fluctuating light environment. second phases, rising metabolite polysome loading drove...
Abstract Plastid protein homeostasis is critical during chloroplast biogenesis and responses to changes in environmental conditions. Proteases molecular chaperones involved plastid quality control are encoded by the nucleus except for catalytic subunit of ClpP, an evolutionarily conserved serine protease. Unlike its Escherichia coli ortholog, this protease essential cell viability. To study function, we used a recently developed system repressible gene expression alga Chlamydomonas...
The vesicle-inducing protein in plastids (VIPP1) was suggested to play a role thylakoid membrane formation via vesicles. As this functional assignment is under debate, we investigated the function of VIPP1 Chlamydomonas reinhardtii. Using immunofluorescence, localized distinct spots within chloroplast. In VIPP1-RNA interference/artificial microRNA cells, consistently observed aberrant, prolamellar body-like structures at origin multiple layers, which appear coincide with immunofluorescent...
Developmental or metabolic changes in chloroplasts can have profound effects on the rest of plant cell. Such intracellular responses are associated with signals that originate and convey information their physiological status to nucleus, which leads large-scale gene expression (retrograde signaling). A screen designed identify components retrograde signaling resulted discovery so-called genomes uncoupled (gun) mutants. Genetic evidence suggests chloroplast protein GUN1 integrates derived...
We applied a top-down systems biology approach to understand how Chlamydomonas reinhardtii acclimates long-term heat stress (HS) and recovers from it. For this, we shifted cells 25 42°C for 24 h back 25°C ≥8 monitored abundances of 1856 proteins/protein groups, 99 polar 185 lipophilic metabolites, cytological photosynthesis parameters. Our data indicate that acclimation HS consists temporally ordered, orchestrated implementation response elements at various system levels. These comprise (1)...
Ectoine is formed in various bacteria as cell protectant against all kinds of stress. Its preservative and protective effects have enabled applications medicine, cosmetics, biotechnology, ectoine therefore has high commercial value. Industrially, produced a complex high-salt process, which imposes constraints on the costs, design, durability fermentation system. Here, Corynebacterium glutamicum upgraded for heterologous production from sugar molasses. To overcome previous limitations,...
Iron deficiency induces a remodeling of the photosynthetic apparatus in Chlamydomonas reinhardtii. In this study we showed that key mechanistic event process photosystem I (PSI) and its associated light-harvesting proteins (LHCI) is N-terminal processing Lhca3. Lhca3 documented independently by two-dimensional gel electrophoresis tandem mass spectrometric (MS/MS) analysis as well quantitative comparative MS/MS peptide profiling using isotopic labeling proteins. Dynamic LHCI complex under...
Abstract Copper response regulator 1 (CRR1), an SBP-domain transcription factor, is a global of nutritional copper signaling in Chlamydomonas reinhardtii and activates genes necessary during periods deficiency. We localized CRR1 to the nucleus mustard (Sinapis alba) seedlings, location consistent with its function as factor. The Zn binding SBP domain binds ions vitro. Cu(I) can replace Zn(II), but Cu(II) form unstable. DNA activity inhibited vitro by or Hg(II) ions, which also prevent...
Crop-plant-yield safety is jeopardized by temperature stress caused the global climate change. To take countermeasures breeding and/or transgenic approaches it essential to understand mechanisms underlying plant acclimation heat stress. this end proteomics are most promising, as largely mediated proteins. Accordingly, several studies, mainly based on two-dimensional gel-tandem MS approaches, were conducted in past. However, results often inconsistent, presumably attributable artifacts...
Summary The Botrytis cinere a VELVET complex regulates light‐dependent development and virulence. goal of this study was to identify common virulence defects several mutants reveal their molecular basis. Growth, differentiation, physiology, gene expression infection fungal strains were analyzed, quantitative comparisons in planta transcriptomes secretomes performed. showed reduced release citric acid, the major acid secreted by wild‐type, whereas no significant role for oxalic observed....
The productivity of plants and microalgae needs to be increased feed the growing world population promote development a low-carbon economy. This goal can achieved by improving photosynthesis via genetic engineering. In this study, we have employed Modular Cloning strategy overexpress Calvin-Benson cycle (CBC) enzyme sedoheptulose-1,7 bisphosphatase (SBP1) up 3-fold in unicellular green alga Chlamydomonas reinhardtii. protein derived from nuclear transgene represented ~0.3% total cell...
Abstract How transcription factors affect chromatin structure to regulate gene expression in response changes environmental conditions is poorly understood the green lineage. To shed light on this issue, we used immunoprecipitation and formaldehyde-assisted isolation of regulatory elements investigate at target genes HSF1 CRR1, key transcriptional regulators heat shock copper starvation responses, respectively, unicellular alga Chlamydomonas reinhardtii. Generally, detected lower nucleosome...
Members of the twin Cx9C protein family constitute largest group proteins in intermembrane space (IMS) mitochondria. Despite their conserved nature and essential role biogenesis respiratory chain, molecular function is largely unknown. We performed a SILAC-based quantitative proteomic analysis to identify interaction partners Cox19. found that Cox19 interacts dynamic manner with Cox11, copper transfer facilitates metalation Cu(B) center subunit 1 cytochrome c oxidase. The Cox11 critical for...
Tim17 is a central, membrane-embedded subunit of the mitochondrial protein import machinery. In this study, we show that contains pair highly conserved cysteine residues form structural disulfide bond exposed to intermembrane space (IMS). This critical for efficient translocation through TIM23 complex and dynamic gating its preprotein-conducting channel. The in formed during insertion into inner membrane. Whereas depends on binding IMS Mia40, oxidoreductase activity Mia40 surprisingly...
For modeling approaches in systems biology, knowledge of the absolute abundances cellular proteins is essential. One way to gain this use quantification concatamers (QconCATs), which are synthetic consisting proteotypic peptides derived from target be quantified. The QconCAT protein labeled with a heavy isotope upon expression E. coli and known amounts purified spiked into whole cell extract. Upon tryptic digestion, unlabeled released native proteins, respectively, both quantified by...
During their first year of growth, overwintering biennial plants transport Suc through the phloem from photosynthetic source tissues to storage tissues. In second year, they mobilize carbon these fuel new growth and reproduction. However, both mechanisms driving this shift link reproductive remain unclear. vegetative sugar beet (
Abstract Background There is increasing evidence that Parkinson’s disease (PD) might start in the gut, thus involving and compromising also enteric nervous system (ENS). At clinical onset of majority dopaminergic neurons midbrain already destroyed, so lack early biomarkers for represents a major challenge developing timely treatment interventions. Here, we use transgenic A30P-α-synuclein-overexpressing PD mouse model to identify appropriate candidate markers gut before hallmark symptoms...
Abstract According to their lifestyle, plant pathogens are divided into biotrophic and necrotrophic organisms. Biotrophic exclusively nourish living host cells, whereas rapidly kill cells cell walls contents. To this end, the fungus Botrytis cinerea secretes large amounts of phytotoxic proteins wall-degrading enzymes. However, precise role these during infection is unknown. Here, we report on identification characterization previously unknown toxic protein hypersensitive response-inducing 1...
Metabolic engineering of microalgae offers a promising solution for sustainable biofuel production, and rational design strategies can be improved by employing metabolic models that integrate enzyme turnover numbers. However, the coverage numbers Chlamydomonas reinhardtii, model eukaryotic microalga accessible to engineering, is 17-fold smaller compared heterotrophic cell factory Saccharomyces cerevisiae. Here we generate quantitative protein abundance data covering 2337 3708 proteins in...
Abstract Thiol-based redox regulation is a crucial posttranslational mechanism to acclimate plants changing light availability. Here, we conducted biotin switch–based proteomics study in Arabidopsis (Arabidopsis thaliana) systematically investigate dynamics of thiol–redox networks response temporal changes availability and across genotypes lacking parts the thioredoxin (Trx) or NADPH-Trx-reductase C (NTRC) systems chloroplast. Time-resolved revealed led marked decreases oxidation states many...