A5020093314- Ion Channels and Receptors
- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Mosquito-borne diseases and control
- Neuroinflammation and Neurodegeneration Mechanisms
- Nitric Oxide and Endothelin Effects
- Adenosine and Purinergic Signaling
- Biochemical Analysis and Sensing Techniques
- Cardiac electrophysiology and arrhythmias
- Phytochemicals and Antioxidant Activities
- Receptor Mechanisms and Signaling
- Malaria Research and Control
- Lipid Membrane Structure and Behavior
- Neuroscience and Neuropharmacology Research
- Cholesterol and Lipid Metabolism
- Viral Infections and Vectors
- Calcium signaling and nucleotide metabolism
- Cellular transport and secretion
- Sirtuins and Resveratrol in Medicine
- Advanced Multi-Objective Optimization Algorithms
- Toxoplasma gondii Research Studies
- Advanced Chemical Sensor Technologies
- Cancer, Hypoxia, and Metabolism
- Steroid Chemistry and Biochemistry
- Erythrocyte Function and Pathophysiology
Indian Institute of Technology BHU
2023
Banaras Hindu University
2023
University of California, Davis
2016-2019
KU Leuven
2010-2019
VIB-KU Leuven Center for Brain & Disease Research
2019
Laboratoire d'Excellence Canaux Ioniques d'Intérêt Thérapeutique
2015
Manipal Hospital
2011
Stempeutics (India)
2011
National Brain Research Centre
2008-2010
ICMR-National Institute of Virology
2009
Reduced functional bladder capacity and concomitant increased micturition frequency (pollakisuria) are common lower urinary tract symptoms associated with conditions such as cystitis, prostatic hyperplasia, neurological disease, overactive syndrome. These can profoundly affect the quality of life afflicted individuals, but available pharmacological treatments often unsatisfactory. Recent work has demonstrated that cation channel TRPV4 is highly expressed in urothelial cells plays a role...
Japanese encephalitis (JE) is the leading form of viral in Asia. It caused by JE virus (JEV), which belongs to family Flaviviridae. JEV endemic many parts Asia, where periodic outbreaks take hundreds lives. Despite catastrophes it causes, has remained a tropical disease uncommon West. With rapid globalization and climatic shift, started emerge areas threat was previously unknown. Scientific evidence predicts that will soon become global pathogen cause worldwide pandemics. Although some...
Background Benzo[a]pyrene (B[a]P) belongs to a class of polycyclic aromatic hydrocarbons that serve as micropollutants in the environment. B[a]P has been reported probable carcinogen humans. Exposure can take place by ingestion contaminated (especially grilled, roasted or smoked) food water, inhalation polluted air. There are reports available also suggests neurotoxicity result exposure, but exact mechanism action is unknown. Methodology/Principal Findings Using neuroblastoma cell line and...
Spinal cord injury (SCI) occurs in the most productive part of life. Treatment options for treatment chronic SCI are few and have limited impact on clinical outcome. Central nervous system (CNS) has intrinsic regeneration capability. The study included patients with complete SCI. Previously harvested autologous mesenchymal stem cells were administered at site after a laminectomy. Follow-up was done by neutral examiner not involved surgery every 3 months. One patient had improvement motor...
The transient receptor potential (TRP) channel TRPM3 is a calcium-permeable cation activated by heat and the neurosteroid pregnenolone sulfate (PregS). highly expressed in sensory neurons, where it plays key role sensing inflammatory hyperalgesia, pancreatic β cells, its activation enhances glucose-induced insulin release. However, despite functional importance, little known about cellular mechanisms that regulate activity. Here, we provide evidence for dynamic regulation of membrane...
The cation channel TRPA1 transduces a myriad of noxious chemical stimuli into nociceptor electrical excitation and neuropeptide release, leading to pain neurogenic inflammation. Despite emergent evidence that is regulated by the membrane environment, it remains unknown whether this localizes in microdomains or interacts with cholesterol. Using total internal reflection fluorescence microscopy density gradient centrifugation we found mouse preferably cholesterol-rich domains functional...
Prevailing dogma holds that activation of the β-adrenergic receptor/cAMP/protein kinase A signalling pathway leads to enhanced L-type CaV 1.2 channel activity, resulting in increased Ca2+ influx into ventricular myocytes and a positive inotropic response. However, full mechanistic molecular details underlying this phenomenon are incompletely understood. clusters decorate T-tubule sarcolemmas myocytes. Within clusters, nanometer proximity between channels permits -dependent co-operative...
Elevated blood glucose (hyperglycemia) is a hallmark metabolic abnormality in diabetes. Hyperglycemia associated with protein kinase A (PKA)-mediated stimulation of L-type Ca2+ channels arterial myocytes resulting increased vasoconstriction. However, the mechanisms by which activates PKA remain unclear. Here, we showed that elevating extracellular stimulates cAMP production myocytes, and this was specifically dependent on adenylyl cyclase 5 (AC5) activity. Super-resolution imaging suggested...
4-Methylnitrosamino-1-(3-pyridyl)-1-butanone (NNK) is a tobacco-specific procarcinogen. We have investigated whether NNK causes inflammatory upheaval in the brain by activation of resident microglia and astrocyte result bystander neuronal damage. carried out work both vitro vivo models. found that treatment with significant mouse microglial (BV2) cell line as evident increase reactive oxygen species nitric oxide level. Western blot analysis has showed proinflammatory signaling proteins,...
Abstract The cation channel TRPM8 plays a central role in the somatosensory system, as key sensor of innocuously cold temperatures and cooling agents. Although increased functional expression has been implicated various forms pathological hypersensitivity, little is known about cellular molecular mechanisms that determine abundance at plasma membrane. Here we demonstrate constitutive transport towards membrane atypical, non-acidic vesicles contain lysosomal-associated protein 1 (LAMP1),...
Large-conductance Ca
Significance Dissemination of chemical information throughout the cell is a fundamental biological process with clinical relevance. Pathological changes in local signaling enzyme activity are linked to diseases, including schizophrenia, Alzheimer’s disease, cardiac arrhythmias, and seizures. Mining patient datasets has uncovered genetic variation A-kinase anchoring proteins (AKAPs) that promotes mislocalization protein kinase A (PKA). We investigate 42 SNPs AKAPs interrupt association PKA...
Elevated glucose increases vascular reactivity by promoting L-type Ca
L-type CaV1.2 channels are key regulators of gene expression, cell excitability and muscle contraction. organize in clusters throughout the plasma membrane. This channel organization has been suggested to contribute concerted activation adjacent (e.g. cooperative gating). Here, we tested hypothesis that dynamic intracellular perimembrane trafficking is critical for formation dissolution functional mediating gating. We found moves vesicular structures circular tubular shape with diverse...
Various TRP channels act as polymodal sensors of thermal and chemical stimuli, but the mechanisms whereby ligands impact on channel gating are poorly understood. Here we show that AITC (allyl isothiocyanate; mustard oil) menthol represent two distinct types at mammalian cold sensor TRPM8. Kinetic analysis revealed acts by destabilizing closed channel, whereas stabilizes open relative to transition state. Based these differences, classify agonists either type I (menthol-like) or II...
The low immunogenicity of neural stem/progenitor cells (NSPCs) coupled with negligible expression MHC antigens has popularized their use in transplantation medicine. However, an inflammatory environment, the NSPCs express costimulatory molecules and antigens, also exhibit certain immunomodulatory functions. Since are cellular targets a number virus infections both during postnatal adult stages, we wanted to investigate immunological properties these stem response viral pathogen.We utilized...
Abstract Japanese encephalitis virus (JEV) induces an acute infection of the central nervous system, pathogenic mechanism which is not fully understood. To investigate host response to JEV infection, 14‐day‐old mice were infected via extraneural route, resulted in and death. Mice that received immune splenocyte transfer protected from infection. Pathology gene expression profiles then compared brains either succumbed or by cell transfer. undergoing progressive had increased proinflammatory...
Transient Receptor Potential (TRP) channels form a broadly expressed and functionally diverse family of cation involved in various (patho)physiological processes. Whereas the mechanisms that control opening TRP have been extensively studied, little is known about transport processes to within plasma membrane. Here we used Total Internal Reflection - Fluorescence Recovery after Photobleaching (TIR-FRAP) selectively visualize bleach fluorescently labeled TRPV2 TRPM4 close proximity...