A5024912561- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Ion Channels and Receptors
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- HER2/EGFR in Cancer Research
- Neurobiology and Insect Physiology Research
- Postharvest Quality and Shelf Life Management
- Immune cells in cancer
- Phytochemicals and Antioxidant Activities
- Respiratory and Cough-Related Research
- Ion channel regulation and function
- Diabetes and associated disorders
- Phagocytosis and Immune Regulation
- Cardiac electrophysiology and arrhythmias
- Biochemical Analysis and Sensing Techniques
- Neuroblastoma Research and Treatments
- Herbal Medicine Research Studies
- Cancer therapeutics and mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Lipid Membrane Structure and Behavior
- Insect Utilization and Effects
VIB-KU Leuven Center for Brain & Disease Research
2018-2021
Ablynx (Belgium)
2021
Center of Molecular Immunology (Cuba)
2008-2019
KU Leuven
2015-2019
International Centre for Genetic Engineering and Biotechnology
2007-2015
University of Havana
2012
Centro de Ingeniería Genética y Biotecnología
2012
Applied Scientific Research (United States)
2012
Overexpression of the epidermal growth factor (EGF) receptor (EGFR) in cancer cells correlates with tumor malignancy and poor prognosis for patients. For this reason, EGFR has become one main targets anticancer therapies. Structural data obtained last few years have revealed molecular mechanism ligand-induced dimerization subsequent signal transduction, also how is blocked by either monoclonal antibodies or small molecules. Nimotuzumab (also known as h-R3) a humanized antibody that been...
Lipopolysaccharides (LPS), the major components of wall gram-negative bacteria, trigger powerful defensive responses in airways via mechanisms thought to rely solely on Toll-like receptor 4 (TLR4) immune pathway. Here we show that airway epithelial cells display an increase intracellular Ca2+ concentration within seconds LPS application. This response occurs a TLR4-independent manner, activation transient potential vanilloid cation channel (TRPV4). We found TRPV4 mediates immediate...
Detecting pathogens and mounting immune responses upon infection is crucial for animal health. However, these come at a high metabolic price (McKean Lazzaro, 2011, Kominsky et al., 2010), avoiding before may be advantageous. The bacterial endotoxins lipopolysaccharides (LPS) are important system cues (Abbas 2014), but it remains unknown whether animals possess sensory mechanisms to detect them prior infection. Here we show that Drosophila melanogaster display strong aversive LPS gustatory...
The cation channel TRPA1 transduces a myriad of noxious chemical stimuli into nociceptor electrical excitation and neuropeptide release, leading to pain neurogenic inflammation. Despite emergent evidence that is regulated by the membrane environment, it remains unknown whether this localizes in microdomains or interacts with cholesterol. Using total internal reflection fluorescence microscopy density gradient centrifugation we found mouse preferably cholesterol-rich domains functional...
Abstract Background Due to its extremely high strength, the interaction between biotin and (strept)avidin has been exploited for a large number of biotechnological applications. Site-specific biotinylation proteins in vivo can be achieved by co-expressing mammalian cells protein interest fused 15 amino acid long Biotin Acceptor Peptide (BAP) bacterial biotin-protein ligase BirA, which specifically recognizes attaches single lysine residue BAP sequence. However, this system is mainly based on...
Subendothelial retention of proatherogenic lipoproteins by proteoglycans is critical in atherosclerosis. The aim this study was to characterize the recognition and antiatherogenic properties a chimeric monoclonal antibody (mAb) that reacts with sulfated molecules.chP3R99 mAb recognized glycosaminoglycans, mainly chondroitin sulfate (CS), ELISA. This blocked ≈70% low-density lipoprotein (LDL)-CS association ≈80% LDL oxidation vitro, when intravenously injected Sprague-Dawley rats (n=6, 1...
Tobacco etch virus protease (TEVp) is a unique endopeptidase with stringent substrate specificity. TEVp has been widely used as purified protein for in vitro applications, but also biological tool directly expressing it living cells. To adapt the to diverse several mutants different stability and enzymatic properties have reported. Herein we describe development of novel engineered mutant designed be active secretory pathway. While wild type targeted pathway mammalian cells synthetized an...
In addition to alterations concerning the expression of oncogenes and onco-suppressors, melanoma is characterized by presence distinctive gangliosides (sialic acid carrying glycosphingolipids). Gangliosides strongly control cell surface dynamics signaling; therefore, it could be assumed that these are linked modifications behavior acquired tumor. On bases, this work investigated correlations between ganglioside metabolism profiles biological features tumor survival patients. Melanoma lines...
Psoriasis is a chronic inflammatory disease with prevalence of approximately 2–3% in the general population. The majority diagnosed patients have plaque psoriasis, and about 20% moderate-to-severe disease. Itolizumab, new monoclonal antibody specific for CD6 molecule mainly expressed on T lymphocytes, has demonstrated to inhibit vitro ligand-induced proliferation pro-inflammatory cytokine production. We assessed immunological histopathological effect using clinical samples taken from 26...
Abstract The GM3(Neu5Gc) ganglioside represents a tumor-specific antigen that is considered promising target for cancer immunotherapy. We previously demonstrated the humanized antibody 14F7hT, specific this ganglioside, exhibited significant antitumor effects in preclinical hematological tumor models. As recognizes human tissues from several origins, we addressed its potential effect on different types. use of cell lines testing GM3(Neu5Gc)-targeting strategies, particular malignancies,...
Gangliosides containing the N-glycolyl (NGc) form of sialic acid are tumor-associated antigens and promising candidates for cancer therapy. We previously generated murine 14F7 monoclonal antibody (mAb), specific N-glycolyl-GM3 ganglioside (NGcGM3), which induced an oncosis-like type cell death on malignant lines expressing this antigen recognized breast carcinoma by immunoscintigraphy in patients. As humanization is expected to enhance its use human therapy, herein we describe design...
Idiotype (Id)-based immunotherapy has been exploited as cancer treatment option. Conceived therapy for malignancies bearing idiotypic antigens, it also extended to solid tumours because of the capacity anti-idiotypic antibodies mimick Id-unrelated antigens. In both these two settings, efforts are being made overcome poor immune responsiveness often experienced when using self immunoglobulins immunogens. Despite a unique gene combination, and thus particular epitopes, is normally difficult...
Abstract B-1 lymphocytes comprise a unique subset of B cells that differ phenotypically, ontogenetically and functionally from conventional B-2 cells. A frequent specificity the antibody repertoire peritoneal is phosphatidylcholine. Liposomes containing phosphatidylcholine have been studied as adjuvants their interaction with dendritic macrophages has demonstrated. However, role in adjuvanticity liposomes composed not explored. In present work, we contribution to humoral response against...
The CD20 molecule is a non-glycosylated protein expressed mainly on the surface of B lymphocytes. In some pathogenic cells, it shows an increased expression, thus becoming attractive target for diagnosis and therapy. Rituximab chimeric antibody that specifically recognizes human molecule. This indicated treatment non-Hodgkin lymphomas autoimmune diseases, such as rheumatoid arthritis systemic lupus erythematosus. this work, we describe stable expression biological evaluation anti-CD20...
Abstract Background and purpose The citrus flavanone hesperetin has been proposed for the treatment of several human pathologies, but its cardiovascular actions remain largely unexplored. Here, we evaluated effect on cardiac electrical contractile activities, aortic contraction, wild‐type voltage‐gated Na V 1.5 channel, a channel mutant (R1623Q) associated with lethal ventricular arrhythmias in long QT syndrome type 3 (LQT3). Experimental approach We used surface ECG contraction force...
P3 monoclonal antibody (MAb) is a murine IgM that specifically recognizes N-glycolyl (NeuGc)-gangliosides and sulfatides. It also reacts with antigens expressed in human breast tumors melanoma. In syngeneic model, MAb able to elicit strong anti-idiotypic (Ab2) response, even the absence of adjuvants or carrier proteins. 1E10 an specific for has demonstrated anti-tumoral effects allogeneic animals. Here we report construction IgG1 chimeric antibodies, evaluation maintenance main properties...