A5017816687- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Pneumonia and Respiratory Infections
- Immune Response and Inflammation
- Platelet Disorders and Treatments
- Bacterial Infections and Vaccines
- Phagocytosis and Immune Regulation
- Immune Cell Function and Interaction
- Blood disorders and treatments
- Glycosylation and Glycoproteins Research
- Marine Invertebrate Physiology and Ecology
- Hemoglobinopathies and Related Disorders
- Complement system in diseases
- Gallbladder and Bile Duct Disorders
- Hepatitis B Virus Studies
- Autoimmune Bullous Skin Diseases
- Antimicrobial Peptides and Activities
- RNA Interference and Gene Delivery
- Neonatal Health and Biochemistry
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Urticaria and Related Conditions
- Marine Ecology and Invasive Species
Canadian Blood Services
2018-2024
St. Michael's Hospital
2016-2023
Unity Health Toronto
2023
University of Havana
2014-2021
University of Toronto
2019
Centro de Ingeniería Genética y Biotecnología
2006-2010
Universitat de Barcelona
2010
Institute for Research in Biomedicine
2010
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2010
National Center for Genetic Engineering and Biotechnology
2009
Cross-presentation is an important mechanism for the differentiation of effector cytotoxic T lymphocytes (CTL) from naïve CD8+ T-cells, a key response clearance intracellular pathogens and tumors. The liposomal co-encapsulation pore-forming protein sticholysin II (StII) with ovalbumin (OVA) (Lp/OVA/StII) induces powerful OVA-specific CTL activation anti-tumor in vivo. However, pathway through which StII contained this preparation able to induce antigen cross-presentation type professional...
Abstract Vaccine strategies to enhance CD8+ CTL responses remain a current challenge because they should overcome the plasmatic and endosomal membranes for favoring exogenous Ag access cytosol of APCs. As way avoid this hurdle, sticholysin (St) II, pore-forming protein from Caribbean Sea anemone Stichodactyla helianthus, was encapsulated with OVA into liposomes (Lp/OVA/StII) assess their efficacy induce response. OVA-specific T cells transferred mice immunized Lp/OVA/StII experienced greater...
Abstract B-1 lymphocytes comprise a unique subset of B cells that differ phenotypically, ontogenetically and functionally from conventional B-2 cells. A frequent specificity the antibody repertoire peritoneal is phosphatidylcholine. Liposomes containing phosphatidylcholine have been studied as adjuvants their interaction with dendritic macrophages has demonstrated. However, role in adjuvanticity liposomes composed not explored. In present work, we contribution to humoral response against...
An anti-erythrocyte antibody redirects inflammation in multiple mouse models.
Abstract Anti-D prevents hemolytic disease of the fetus and newborn, this mechanism has been referred to as Ab-mediated immune suppression (AMIS). Anti-D, well other polyclonal AMIS-inducing Abs, most often induce both epitope masking erythrocyte clearance mechanisms. We have previously observed that some Abs successfully AMIS effects could be split into those mediate versus clearance, allowing ability analyze these mechanisms separately. In addition, activity recently shown Ag modulation...
Fc gamma receptors (FcγRs) are critical effector for immunoglobulin G (IgG) antibodies. On macrophages, FcγRs mediate multiple functions, including phagocytosis, but the individual contribution of specific to phagocytosis has not been fully characterized. Primary human macrophage populations, such as splenic can express FcγRI, FcγRIIA, and FcγRIIIA. However, there is currently no widely available monocyte or cell line expressing all these receptors. Common sources monocytes differentiation...
Abstract Background Despite the vast antigen disparity between donor and recipient red blood cells (RBCs), only 2%–6% of transfusion patients mount an alloantibody response. Recently, RBC density has been proposed as one factors that can influence alloimmunization, however, there no characterization role along with dose in primary secondary immunization. Study Design Methods To generate RBCs express distinct copy numbers, different quantities hen egg lysozyme (HEL) were coupled to murine...
Abstract Background The administration of anti‐D for the prevention hemolytic disease fetus and newborn is one most successful clinical uses phenomenon antibody‐mediated immune suppression (AMIS). However, despite adequate prophylaxis, failures can still occur in clinic are poorly understood. Recently, copy number red blood cell (RBC) antigens has been shown to influence immunogenicity context RBC alloimmunization; however, its on AMIS remains unexplored. Study Design Methods RBCs expressing...
Abstract Fc gamma receptor (FcγR) IIIA is an important for immunoglobulin G (IgG) and involved in immune defense mechanisms as well tissue destruction some autoimmune diseases including thrombocytopenia (ITP). FcγRIIIA on macrophages can trigger phagocytosis of IgG-sensitized platelets, prior pilot studies observed blockade increased platelet counts patients with ITP. Unfortunately, although ITP counts, its engagement by the blocking antibody drove serious adverse inflammatory reactions....
Liposomes containing phosphatidylcholine have been widely used as adjuvants. Recently, we demonstrated that B-1 cells produce dipalmitoyl-phosphatidylcholine (DPPC)-specific IgM upon immunization of BALB/c mice with DPPC-liposomes encapsulating ovalbumin (OVA). Although this preparation enhanced the OVA-specific humoral response, contribution anti-DPPC antibodies to effect was unclear. Here, demonstrate these are secreted by independently presence OVA in formulation. We also confirm specific...
The formulation of a broadly protective vaccine to prevent the serogroup B Neisseria meningitidis (MenB) disease is still an unmet medical need. We have previously reported induction bactericidal and antibodies against MenB after immunization mice with phage-displayed peptide named 4 L-5. This mimics capsular polysaccharide (CPS) epitope in MenB. With aim developing formulations that could be used humans, we evaluate this study various forms presentation immune system L-5 sequence, based on...
Antibodies targeting erythrocytes can induce antibody‐mediated inhibition of erythrocyte alloimmunization, and anti‐D has been extremely successful in preventing haemolytic disease the foetus newborn ( HDFN ). It is desirable to replace current donor‐derived with a monoclonal antibody; however, exact mechanism IgG‐mediated suppression red blood cell immune responses remains unclear. proposed that ability prevent due IgG interactions Fc receptors on phagocytic cells leading rapid clearance...
A simple, specific, sensitive and reproducible ELISA has been developed to quantify the level of CPS (capsular polysaccharide) production in supernatants Streptococcus pneumoniae cell cultures. CPSs from Strep. have widely used as vaccine antigens. The quantification method is based on two type-23F serotype-specific polyclonal antibodies: IgG, purified sera mice immunized with a pneumococcal conjugate, coating step, rabbit serum second antibody. Solutions were standards. relationship between...
Immune thrombocytopenia (ITP) is an autoimmune disease characterized by low platelet counts primarily due to antiplatelet autoantibodies. Anti-D a donor-derived polyclonal Ab against the rhesus D Ag on erythrocytes used treat ITP. Unfortunately, adverse inflammatory/hypersensitivity reactions and Food Drug Administration-issued black box warning have limited its clinical use. This underscores imperative understand inflammatory pathway associated with anti-erythrocyte Ab-based therapies....