A5101779898- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Marine Invertebrate Physiology and Ecology
- interferon and immune responses
- Immune Response and Inflammation
- Hedgehog Signaling Pathway Studies
- Cytokine Signaling Pathways and Interactions
- Cancer, Hypoxia, and Metabolism
- Diabetes and associated disorders
- Atherosclerosis and Cardiovascular Diseases
- Marine Toxins and Detection Methods
- Wnt/β-catenin signaling in development and cancer
- Genomics and Rare Diseases
- Genetic and Kidney Cyst Diseases
- Ethics in Clinical Research
- Invertebrate Immune Response Mechanisms
- Immunodeficiency and Autoimmune Disorders
- Mycobacterium research and diagnosis
- Erythrocyte Function and Pathophysiology
- Neurobiology and Insect Physiology Research
- vaccines and immunoinformatics approaches
The University of Texas Rio Grande Valley
2023-2024
Community Care
2024
Universidad de la República
2024
University of Havana
2022-2024
The University of Texas MD Anderson Cancer Center
2023
Universidad de Playa Ancha de Ciencias de la Educación
2022
John Brown University
2022
Center of Molecular Immunology (Cuba)
2012-2021
Brown University
2018
University of Miami
2003-2017
Prostate cancer is the most common solid tumor in men, and it shares with all cancers hallmark of elevated, nonhomeostatic cell proliferation. Here we have tested hypothesis that SONIC HEDGEHOG (SHH)–GLI signaling pathway implicated prostate cancer. We report expression SHH–GLI components adult human cancer, often enhanced levels tumors versus normal prostatic epithelia. Blocking cyclopamine or anti-SHH antibodies inhibits proliferation GLI1 + / PSA primary cultures. Inversely, SHH can...
The traditional definition of natural antibodies (Nabs) states these are present prior to the body encountering cognate antigen providing a first line defense against infection thereby, allowing time for specific antibody response be mounted. literature has seemingly common NAbs; however, as our knowledge and B cells is refined, re-evaluation may required. Defining becomes important function NAb production used define cell subsets (1) molecules shown play numerous roles in immune system...
Abstract Human Ab-secreting cell (ASC) populations in circulation are not well studied. In addition to B-1 (CD20+CD27+CD38lo/intCD43+) and conventional plasmablast (PB) (CD20-CD27hiCD38hi) populations, this study, we identified a novel B population termed 20+38hi cells (CD20+CD27hiCD38hi) that spontaneously secretes Ab. At steady-state, distinct from PBs on the basis of CD20 expression, amount Ab production, frequency mutation, diversity BCR repertoire. However, cytokine treatment induces...
Age-related deficits in the immune system have been associated with an increased incidence of infections, autoimmune diseases and cancer. Human B cell populations change quantitatively qualitatively elderly. However, function human B-1 cells, which play critical anti-microbial housekeeping roles, not studied older age population. In present work, we analyzed how frequency, repertoire peripheral blood cells (CD19+CD20+CD27+ CD38low/intCD43+) age. Our results show that only percentage but also...
Abstract We generated the 1E10 γ-type anti-idiotype mAb (Ab2) specific to an Ab1 able react specifically with N-glycolyl-containing gangliosides and Ags expressed on human melanoma breast carcinoma cells. This Ab2 induced Ab response in animal models sharing immunochemically defined idiotopes Ab1. The treatment of tumor-bearing mice a strong antitumor activity. A clinical trial was conducted 20 patients advanced malignant melanoma. Patients were treated six intradermal injections aluminum...
Abstract Background Genetic studies associated the CAPB locus with familial risk of brain and prostate cancers. We have identified HSPG2 ( Perlecan ) as a candidate gene for CAPB. Previously we linked to Hedgehog signaling in Drosophila . More recently, demonstrated importance humans advanced cancer. Results Here demonstrate expression cancer, its function cancer cell growth through interaction modulation Sonic (SHH) signaling. tissues correlates high Gleason score rapid proliferation. is...
Abstract 1E10 mAb is an anti-Id murine (Ab2 mAb) specific for Ab1 that reacts with NeuGc-containing gangliosides, sulfatides, and Ags expressed in some human tumors. In preclinical studies, this Ab2 Ab was able to mimic gangliosides only animals lacking expression of these normal tissues. study, we report on the immune responses elicited 20 non-small cell lung cancer patients treated 1 mg aluminum hydroxide-precipitated mAb. hyperimmune sera from 16 patients, a strong response both IgM IgG...
1E10 is a murine anti-idiotypic mAb specific for an idiotypic that reacts with NeuGc-containing gangliosides, sulfatides, and Ags expressed in some human tumors. In melanoma, breast, lung cancer patients, this Ab was able to induce response against N-glycosylated attractive targets immunotherapy as these glycolipids are not naturally humans. A clinical study nonsmall cell patients showed encouraging benefits. Immunological studies performed 20 of suggested correlation between the induction...
Drosophila is a powerful model for the study of factors modulating innate immunity. This examines effect water-loss dehydration on immune responsiveness in renal system (Malpighian tubules; MTs), and how this leads to elevated host defense contributes immunosenescence. A short period desiccation-elevated peptidoglycan recognition protein-LC (PGRP-LC) expression MTs, increased antimicrobial peptide (AMP) gene induction, protected animals from bacterial infection. We show that desiccation...
An IgM monoclonal antibody (MAb), named P3, has the characteristic to react specifically with a broad battery of N-glycolyl containing-gangliosides and antigens expressed on breast tumors. When this MAb was administered alone in syngeneic mice, an specific IgG anti-idiotypic (Ab2) response induced, Ab2 increased when P3 injected coupled carrier protein presence Freund's adjuvant. Spleen cells from these mice were used somatic-cell hybridization experiments, using murine myeloma cell line...
N ‐ glycolylated gangliosides are not naturally expressed in healthy human tissues but overexpressed several tumors. We demonstrate the existence of antibodies that bind ( N‐ glycolylneuraminyl)‐lactosylceramide eu G c GM 3) and detectable sera 65 from 100 donors (65%) tested by ELISA . From those 3 antibody‐positive donors, 35 had were able to recognize kill 3‐expressing tumor cells a complement‐mediated mechanism. After complement inactivation, 11 positive showed direct cytotoxic effect on...
While not naturally expressed in normal human tissues, N-glycolylated (NeuGc) gangliosides are overexpressed several tumors and have immunosuppressive capacity, which contributes to cancer progression. Naturally occurring antibodies against NeuGcGM3 exist healthy donors that specifically recognize kill tumor cells expressing the antigen by complement-dependent -independent mechanisms, latter resembling an oncotic necrosis-type of cell death. Both levels anti-NeuGcGM3 sera percentage with...