A5066718032- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Glycosylation and Glycoproteins Research
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Antimicrobial Peptides and Activities
- Animal Genetics and Reproduction
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Veterinary medicine and infectious diseases
- Animal Virus Infections Studies
- Toxin Mechanisms and Immunotoxins
- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Influenza Virus Research Studies
- Transgenic Plants and Applications
- Aquaculture disease management and microbiota
- Bioactive Compounds and Antitumor Agents
- Viral Infections and Outbreaks Research
- vaccines and immunoinformatics approaches
University of Concepción
2015-2024
Universidad de Antioquia
2023
Centro de Ingeniería Genética y Biotecnología
2004-2009
Centro Nacional de Sanidad Agropecuaria
2008
National Institute for Bioprocessing Research and Training
2008
Overexpression of the epidermal growth factor (EGF) receptor (EGFR) in cancer cells correlates with tumor malignancy and poor prognosis for patients. For this reason, EGFR has become one main targets anticancer therapies. Structural data obtained last few years have revealed molecular mechanism ligand-induced dimerization subsequent signal transduction, also how is blocked by either monoclonal antibodies or small molecules. Nimotuzumab (also known as h-R3) a humanized antibody that been...
The main problem in the flotation of Cu-Mo sulfide ores seawater is poor floatability molybdenite at pH>9.5. Froth stability plays a very important role determining concentrate grade and recovery operations this paper both frothability have been tested. studied by measuring froth equilibrium layer thickness modified laboratory cell. Two chemical aspects need to be considered: content NaCl (around 87% salinity), concentration secondary ions 13%) (sulfate, magnesium, calcium, bicarbonate ions,...
Altered expression and function of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) has been associated with several diseases such as endothelial dysfunction, atherosclerosis obesity. In these pathologies, oxLDL/LOX-1 activates signaling pathways that promote cell proliferation, motility angiogenesis. Recent studies have indicated olr1 mRNA is over-expressed in stage III IV human prostatic adenocarcinomas. However, the LOX-1 prostate cancer angiogenesis remains to be...
Nanobodies (Nbs) are single domain antibody fragments derived from heavy-chain antibodies found in members of the Camelidae family. They have become a relevant class biomolecules for many different applications because several important advantages such as their small size, high solubility and stability, low production costs. On other hand, synthetic Nb libraries emerging an attractive alternative to animal immunization selection antigen-specific Nbs. Here, we present design construction new...
Andes virus (ANDV) is a human-pathogenic hantavirus. Hantaviruses presumably initiate their mRNA synthesis by using cap structures derived from host cell mRNAs, mechanism called cap-snatching. A signature for cap-snatching endonuclease present in the N terminus of hantavirus L proteins. In this study, we aimed to solve atomic structure ANDV and characterize its biochemical features. However, wild-type protein was refractory expression Escherichia coli, due toxic enzyme activity. To...
To design and construct a new synthetic nanobody library using structure-based approach that seeks to maintain high protein stability increase the number of functional variants within combinatorial space mutations.Synthetic (Nb) libraries are emerging as an attractive alternative animal immunization for selection stable, affinity Nbs. Two key features define Nb library: framework CDR design. We selected universal VHH from cAbBCII10 Nb. CDR1 CDR2 were designed with same fixed length in...
NK-lysin is a potent antimicrobial peptide (AMP) with activity against bacteria, fungi, viruses, and parasites. type of granulysin, member the saposin-like proteins family first isolated from pig’s small intestine. In previous work, for time, we identified four variants nk-lysin Atlantic salmon ( Salmo salar ) using EST sequences. present study, reported characterized two additional transcripts S. . Besides, evaluated tissue distribution three NK-lysins assessed antimicrobial, hemolytic,...
Human erythropoietin is mainly recognized for its hematopoietic function; however, by binding to receptor (EpoR), it can activate different signaling pathways as STAT, PI3K, MAPK and RAS increase cellular differentiation or provide neuroprotective effects, among others. A recombinant human variant with low glycosylation without effect (EpoL) was purified from skimmed goat milk. Recombinant (Epo) obtained CHO cell line used control compare EpoL effects. Neuroprotection studies were performed...
Over the last few years, several commercial FSH products have been developed for cattle superovulation (SOV) purposes in Multiple Ovulation and Embryo Transfer (MOET) programs. The SOV response is highly variable among individuals remains one of main limiting factors obtaining a profitable number transferable embryos. In this study, follicle stimulating hormone (FSH) from different origins was included two protocols, (a) purified pig pituitary extract (NIH-FSH-p; doses/day, 12 h apart, four...
ABSTRACT Subunit recombinant vaccines against classical swine fever virus (CSFV) are a promising alternative to overcome practical and biosafety issues with inactivated vaccines. One of the strategies in evaluation under field conditions is use new marker E2-based vaccine produced milk adenovirally transduced goats. Previously we had demonstrated efficacy this antigen, which conferred early protection long-lasting immunity CSFV infection. Here, have used simpler downstream process obtain...
The extracellular domain of E2 glycoprotein outer surface the classical swine fever virus was expressed in epithelial kidney pig cells. N-glycosylation determined by combination Normal Phase-HPLC, Weak Anion Exchange-HPLC, exoglycosidase digestions and Mass Spectrometry revealed a complex mixture neutral monosialylated multiantennary N-glycans with variable number alpha1-3-Gal-Gal antennae terminals. most abundant N-glycan has composition Hex(7)HexNAc(4)dHex(1), Negative ion ESI-MS/MS...