A5007159329- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Production and Characterization
- Animal Disease Management and Epidemiology
- Antimicrobial Peptides and Activities
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Veterinary medicine and infectious diseases
- Vector-Borne Animal Diseases
- Biofuel production and bioconversion
- Galectins and Cancer Biology
- Invertebrate Immune Response Mechanisms
- Carbohydrate Chemistry and Synthesis
- Fungal and yeast genetics research
- Enzyme-mediated dye degradation
- Coccidia and coccidiosis research
- Transgenic Plants and Applications
- Protein Hydrolysis and Bioactive Peptides
- Viral Infections and Immunology Research
- Animal Genetics and Reproduction
- Advanced Biosensing Techniques and Applications
- Aquaculture disease management and microbiota
- Entomological Studies and Ecology
University of Concepción
2015-2024
Hospital Regional de Concepción
2020-2022
Centro de Ingeniería Genética y Biotecnología
1999-2012
National Institute for Bioprocessing Research and Training
2008
Centro Nacional de Sanidad Agropecuaria
2008
Uppsala University
1991
La biotecnología molecular es un campo científico transformador con aplicaciones fundamentales en salud, agricultura, medio ambiente e industria. En América Latina, el fortalecimiento de esta disciplina mediante una formación alto nivel esencial para abordar los desafíos regionales más urgentes. Este artículo destaca la importancia estratégica educación doctoral molecular, centrando atención Doctorado Universidad Concepción (UDEC), Chile, como modelo excelencia y accesibilidad. Se analiza...
Abstract We have cloned and expressed a bacterial thermostable alpha amylase gene in Pichia pastoris using the methanol‐controlled alcohol oxidase ( AOX1 ) promoter. Two integrative vectors were constructed with two different secretion signal sequences order to obtain efficient of protein. One vector contains structural encoding mature fused SUC2 sequence from Saccharomyces cerevisiae . In other vector, is its own sequence. both cases, secreted into culture medium high efficiency, around 2·5...
NK-lysin is a potent antimicrobial peptide (AMP) with activity against bacteria, fungi, viruses, and parasites. type of granulysin, member the saposin-like proteins family first isolated from pig’s small intestine. In previous work, for time, we identified four variants nk-lysin Atlantic salmon ( Salmo salar ) using EST sequences. present study, reported characterized two additional transcripts S. . Besides, evaluated tissue distribution three NK-lysins assessed antimicrobial, hemolytic,...
The cDNA of cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium has been cloned and sequenced. 5′ end was obtained by PCR amplification. contains 2310 translated bases excluding the poly(A) tail. deduced mature protein 770 amino acid residues is preceded a 18 residue long signal peptide. regions sequence corresponding to heme FAD domains CDH were identified as well nucleotide‐binding motif, disulfide pairing methionine chelating iron. No homologous sequences found for domain,...
Human erythropoietin is mainly recognized for its hematopoietic function; however, by binding to receptor (EpoR), it can activate different signaling pathways as STAT, PI3K, MAPK and RAS increase cellular differentiation or provide neuroprotective effects, among others. A recombinant human variant with low glycosylation without effect (EpoL) was purified from skimmed goat milk. Recombinant (Epo) obtained CHO cell line used control compare EpoL effects. Neuroprotection studies were performed...
Contact with the urticating setae from abdomen of adult females neo-tropical moth Hylesia metabus gives rise to an dermatitis, characterized by intense pruritus, generalized malaise and occasionally ocular lesions (lepidopterism). The contain a pro-inflammatory glycosylated protease homologous other S1A serine proteases insects. Deglycosylation PNGase F in presence buffer prepared 40% H218O allowed assignment N-glycosylation site. Five main paucimannosidic N-glycans were identified, three...
Using anion‐exchange chromatography the catalyticdomain of endoglucanase 1 (Cel7B) from Trichoderma reesei was resolved in multiple fractions with different isoelectric points, presumably related to glycoforms enzyme. The protein were analysed using lectins and electrospray MS. Isolated N‐glycans by fluorophore‐assisted carbohydrate electrophoresis amine‐adsorption HPLC. results show that this particular preparation contained at least 14 glycoforms. major isoform only one GlcNAc, N‐linked,...
MAbs (monoclonal antibodies) are becoming increasingly important as diagnostic tools for pharmaceutical biotechnology, and hence it is crucial that they produced under controlled conditions to assure their consistency reproducibility, not only in terms of protein sequence bioactivity, but also post-translational modifications, e.g. N-glycosylation. Hybridoma CB.Hep-1, which secretes an IgG2b mAb, was cultured vivo ascites vitro static-flask, spinner-flask, dialysis-membrane perfusion systems...
The extracellular domain of E2 glycoprotein outer surface the classical swine fever virus was expressed in epithelial kidney pig cells. N-glycosylation determined by combination Normal Phase-HPLC, Weak Anion Exchange-HPLC, exoglycosidase digestions and Mass Spectrometry revealed a complex mixture neutral monosialylated multiantennary N-glycans with variable number alpha1-3-Gal-Gal antennae terminals. most abundant N-glycan has composition Hex(7)HexNAc(4)dHex(1), Negative ion ESI-MS/MS...
In the present paper we report biochemical characteristics of recombinant tick (Boophilus microplus) gut antigen Bm86 that previously has been cloned, expressed and recovered at high levels in methylotrophic yeast Pichia pastoris. The results demonstrate rBm86 had a modification position 92 (Thr replaced by Ile) aggregated, forming particles ranging between 17 40 nm. was N‐glycosylated, having least two non‐glycosylated sequons (Asn‐329 Asn‐363) ratio only 0.4/65 (free Cys/total Cys)/mol protein.
Multivalent live-attenuated or inactivated vaccines are often used to control the bovine viral diarrhea disease (BVD). Still, they retain inherent disadvantages and do not provide expected protection. This study developed a new vaccine prototype, including external segment of E2 protein from five different subgenotypes selected after massive screening. The proteins every subgenotype (1aE2, 1bE2, 1cE2, 1dE2, 1eE2) were produced in mammalian cells purified by IMAC. An equimolar mixture...
Interferons (IFNs) are cytokines involved in the immune response with a synergistic regulatory effect on response. They therapeutics for various viral and proliferative conditions, proven safety efficacy. Their clinical application is challenging due to molecules' size, degradation, pharmacokinetics. We working new drug delivery systems that provide adequate therapeutic concentrations these prolong their half-life circulation, such as nanoformulations.
Aspartic protease, widely used as a milk-coagulating agent in industrial cheese production, contains three potential N-glycosylation sites. In this study, we report the characterization of N-linked oligosaccharides on recombinant aspartic protease secreted from methylotrophic yeast Pichia pastoris using combination mass spectrometric, 2D chromatographic, chemical and enzymatic methods. The carbohydrates site I (Asn79) were found to range Man6-17GlcNAc2 with 50% bearing phospho-diester-motif,...
Previously, we designed a subunit vaccine candidate based on three L. intracellularis antigens with promising results in pigs. In this study, were produced individually to achieve an even antigen ratio the formulation. The emulsion characterization included drop size and mechanical thermal stability. Immune response was evaluated by indirect sandwich ELISAs, qPCR, flow cytometry. candidate's safety assessed histopathology monitoring clinical behavior of animals. average production yielded...