A5072719603- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Viral gastroenteritis research and epidemiology
- Hepatitis C virus research
- Viral Infectious Diseases and Gene Expression in Insects
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Nanoparticle-Based Drug Delivery
- Glycosylation and Glycoproteins Research
- Obsessive-Compulsive Spectrum Disorders
- Streptococcal Infections and Treatments
- Advanced Biosensing Techniques and Applications
- Cell Adhesion Molecules Research
- Genetics, Bioinformatics, and Biomedical Research
- Galectins and Cancer Biology
- Vitamin C and Antioxidants Research
- Educational methodologies and cognitive development
- Dementia and Cognitive Impairment Research
- Advanced biosensing and bioanalysis techniques
- Protease and Inhibitor Mechanisms
- Molecular Biology Techniques and Applications
- Health and Medical Education
- Antioxidant Activity and Oxidative Stress
- Eicosanoids and Hypertension Pharmacology
- Carcinogens and Genotoxicity Assessment
University of Concepción
2019-2024
Cuban Neuroscience Center
2003-2020
Multivalent live-attenuated or inactivated vaccines are often used to control the bovine viral diarrhea disease (BVD). Still, they retain inherent disadvantages and do not provide expected protection. This study developed a new vaccine prototype, including external segment of E2 protein from five different subgenotypes selected after massive screening. The proteins every subgenotype (1aE2, 1bE2, 1cE2, 1dE2, 1eE2) were produced in mammalian cells purified by IMAC. An equimolar mixture...
Interferons (IFNs) are cytokines involved in the immune response with a synergistic regulatory effect on response. They therapeutics for various viral and proliferative conditions, proven safety efficacy. Their clinical application is challenging due to molecules' size, degradation, pharmacokinetics. We working new drug delivery systems that provide adequate therapeutic concentrations these prolong their half-life circulation, such as nanoformulations.
Abstract Tumor necrosis factor‐alpha (TNFα) inhibitors could prevent neurological disorders systemically, but their design generally relies on molecules unable to cross the blood–brain barrier (BBB). This research was aimed and characterize a novel TNFα inhibitor based angiopeptide‐2 as BBB shuttle molecule fused extracellular domain of human receptor 2 mutated vascular endothelial growth factor (VEGF) dimerization domain. new chimeric protein (MTV) would be able trigger receptor‐mediated...
Background: Apolipoprotein E (ApoE) ∊4 genotype is the most clearly documented risk factor for Alzheimer’s disease (AD). Epidemiological studies demonstrate an accelerated rate of progression to dementia and AD in patients with mild cognitive impairment (MCI). We assessed ApoE allele genotypes frequencies Cuban MCI. Methods: performed genotyping 74 more than 65 years old. Cognitive assessments included Mini-Mental State Examination (MMSE) a battery evaluating memory, attention, perception,...
Introduction: Group A streptococci (GAS) is responsible of several human diseases ranging from mild infection to severe invasive toxin-mediated disease and post-infectious sequelae. Accordingly, a GAS surveillance program based on molecular techniques advisable for its epidemiological control. Pulsed-field gel electrophoresis (PFGE) the gold standard subtyping, but major disadvantage length procedure, which takes 1–3days work, minimum. The aim this study was develop rapid cost-effective...
Interferons (IFNs) are cytokines involved in the immune response with synergistic regulatory action. They therapeutics for various viral and proliferative conditions, proven safety efficacy. Their clinical application presents difficulties due to molecules' size, degradation, pharmacokinetics. We developed a controlled release system respiratory tract infections. A core-shell nanoparticle was prototyped, which hydrolyzed shell (polyvinylpyrrolidone), releasing active ingredients...
: Oxidized low-density lipoprotein (ox-LDL) is a proinflammatory particle associated with various diseases and affects cell proliferation viability in multiple types. However, its impact on intestinal epithelial cells remains underexplored. This study investigates the effect of ox-LDL colonic viability, as well underlying mechanisms involved.
Effective drug delivery to the central nervous system (CNS) remains a challenge due blood–brain barrier (BBB). Macromolecules such as proteins and peptides are unable cross BBB have poor therapeutic efficacy little or no distribution. A promising alternative is conjugation of shuttle molecule that can reach CNS via receptor-mediated transcytosis (RMT). Several receptors been described for RMT, low-density lipoprotein receptor-related protein 1 (LRP1). We used phage display technology...
The neutralization of tumor necrosis factor alpha (TNFα) with biopharmaceuticals is a successful therapy for inflammatory diseases. Currently, one the main TNFα-antagonists Etanercept, dimeric TNF-R2 ectodomain. Considering that TNFα and its receptors are homotrimers, we proposed trimeric ectodomain could be an innovative TNFα-antagonist. Here, 3cTNFR2 protein was designed by fusion collagen XV trimerization domain. produced in HEK293 cells purified immobilized metal affinity chromatography....
Abstract In the present work, a comparative study of 5-FdUrd, thy -, and metabolic in vivo labeling methods for plasmid chromosomal DNA E. coli DH5α cells was performed order to achieve best thymidine substitution method by 5-BrdUrd. According colorimetric immunoenzymatic results, we found that minimal detectable labelled (MDLD) 312 pg with 5-FdUrd - 5-BrdUrd DNA. replaced about 96% total methods; methods, MDLD value 1,25 ng denatured Pyrimidine nucleoside analogues were also evaluated as...
Mild cognitive impairment (MCI) is defined as a state of the ageing brain midway between normal cognition and dementia. Special attention has been paid to electrophysiological substrate underlying Alzheimer's disease MCI in order identify early possible which subjects with progress towards Alzheimer, could be crucial for starting rehabilitation or pharmacological therapies.To perform spectral characterisation electroencephalogram MCI.An was carried out on 41 analyse measurements;...
Abstract DNA molecules suitable for amplification by Polymerase Chain Reaction were obtained immobilizing whole blood or isolated leukocytes and incubating the immobilized cells one hour with known non‐enzymatic solution described preparing intact PFGE. Cell immobilization was done in agarose gels punches of 1.2 mm diameter had amount needed amplifying chromosomal mitochondrial sequences, many times. The approach successfully used from multiple samples flat‐bottom 96‐well ELISA plates....
The tumor necrosis factor alpha (TNFα) is involved at multiple stages of Alzheimer's disease pathophysiology and progression, emerging the TNFα inhibition as a therapeutic alternative. However, large sizes biological inhibitors impede its cross through blood-brain barrier (BBB), therefore therapy application. Here, we report novel inhibitor protein, MTH-TNFR2-VEGF*, obtained by fusion BBB penetrating peptide (Molecular Trojan Horse- MTH) with TNF receptor II extracellular domain mutated VEFG...
Introduction. It has been hypothesized that pediatric autoimmune neuropsychiatric disorder associated with streptococcal infections (PANDAS) etiology results from an abnormal immune response to infection. There is evidence the serotonergic system involved in both obsessive-compulsive (OCD) physiopathology and immunological processes. In 5’ promoter region of 5-HTT, gene encoding for serotonin transporter we can find 5-HTTLPR polymorphism OCD. Being PANDAS a OCD symptoms likely abnormalities,...