A5005100749- Monoclonal and Polyclonal Antibodies Research
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Bacterial Infections and Vaccines
- Viral Infectious Diseases and Gene Expression in Insects
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- vaccines and immunoinformatics approaches
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Hepatitis Viruses Studies and Epidemiology
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Protein purification and stability
- Virology and Viral Diseases
- Escherichia coli research studies
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Pediatric health and respiratory diseases
- Toxin Mechanisms and Immunotoxins
- CRISPR and Genetic Engineering
- Hepatitis B Virus Studies
- Immunodeficiency and Autoimmune Disorders
University of Concepción
2015-2025
Universidad de Antioquia
2023
Finlay Institute
2006-2019
Centro Nacional de Microbiologia
2019
Instituto de Salud Carlos III
2019
Centro de Investigación Biomédica en Red
2019
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2019
Hôpital de la Conception
2018
University of Havana
2015
Abstract Background Immunoglobulin A is the most abundant isotype in secretions from mucosal surfaces of gastrointestinal, respiratory and genitourinary tracts external such as colostrum, breast milk, tears saliva. The high concentration human secretory IgA (hsIgA) colostrum strongly suggests that it should play an important role passive immune protection against gastrointestinal infections. Materials methods Human was purified colostrum. reactivity hsIgA mycobacterial antigens its...
Nanobodies (Nbs) are single domain antibody fragments derived from heavy-chain antibodies found in members of the Camelidae family. They have become a relevant class biomolecules for many different applications because several important advantages such as their small size, high solubility and stability, low production costs. On other hand, synthetic Nb libraries emerging an attractive alternative to animal immunization selection antigen-specific Nbs. Here, we present design construction new...
A recombinant peptide as a TNF-blocking agent was obtained using fermentation in E. coli. The strategy based on the expression of multimeric tandem protein comprising 15 repeated copies r-CBB288 peptide, and monomeric unit released with hydroxylamine-assisted proteolysis step. At this point, size exclusion chromatography used to separate monomer from unreacted polymer forms. Therefore, main theme study develop phenomenological General Rate Model describe elution profile SEC ensure required...
Tuberculosis (TB) is one of the most important causes mortality and morbidity due to infectious diseases. BCG, vaccine in use, not fully protective against TB. In a previous study, we have shown that proteoliposomes (outer membrane extracts), obtained from BCG (PLBCG) were able induce humoral immune responses Mycobacterium tuberculosis (Mtb) antigens. With objective evaluate capability PLBCG alone or as booster with murine model progressive pulmonary TB was used. Animals immunized adjuvanted...
Abstract The development of molecules specific for M. tuberculosis -infected cells has important implications, as these tools may facilitate understanding the mechanisms regulating host pathogen interactions in vivo . In addition, new capable to targeting have potential applications diagnosis, treatment, and prevention (TB). Due lack CD1b polymorphism, lipid-CD1b complexes could be considered universal infection markers. aim present study was display on PIII surface protein m13 phage, a...
TNFα is a pro-inflammatory cytokine that therapeutic target for inflammatory autoimmune disorders. Thus, antagonists are successfully used the treatment of these Here, new association patterns rhTNFα and its Adalimumab Etanercept disclosed. Active was purified by IMAC from soluble fraction transformed Escherichia coli. Protein detection assessed SDS–PAGE Western blot. The KD values interactions with their were obtained non-competitive ELISA microscale thermophoresis (MST). Molecular sizes...
Abstract Currently, the generation of cell lines for production recombinant proteins has limitation unstable gene expression due to repeat-induced silencing or loss transgene copies resulting from recombination events. In this work, we developed a new strategy based on sequential insertion transgenes generating stable clones producing high levels chimeric human follicle-stimulating hormone (hscFSH). Gene was done by transducing HEK-293 cells with lentiviral vector containing bicistronic...
The development of new tools capable targeting Mycobacterium tuberculosis (Mtb)-infected cells have potential applications in diagnosis, treatment, and prevention tuberculosis. In Mtb-infected cells, CD1b molecules present Mtb lipids to the immune system (Mtb lipid-CD1b complexes). Because lack polymorphism, specific complexes could be considered as universal infection markers. 2-Stearoyl-3-hydroxyphthioceranoyl-2'-sulfate-α-α'-d-trehalose (Ac2SGL) is for Mtb, not other mycobacterial...
The availability of random peptide libraries displayed on bacteriophage (RPL) has provided a powerful tool for selecting sequences that mimic binding properties natural antigen epitopes (mimotopes). These mimotopes can be used vaccine design, drug development, and diagnostic assays. Several have been shown to induce production antibodies against the antigen. We previously identified four dengue virus from phage-displayed library using antidengue 3 human sera. Three them showed similarity in...
The human erythropoietin (hEPO) is the main hormone concerned with stimulation of erythropoiesis and maintain erythroid stem cells physiologic levels.Its deficiency causes severe anemia happens to patients kidney failure because traumas, graft, chemotherapy, diabetes, etc. Hormone supply an essential treatment on these patients.Currently, recombinant (rhEPO) mainly produced in genetically modified CHO cells, regardless high cost, those other cell lines or organisms have different patterns...
Hantavirus Pulmonary Syndrome (HPS), caused by the Andes virus (ANDV), is a significant public health concern in South America, with no available vaccines or specific treatments. Neutralizing antibodies targeting ANDV glycoproteins Gn and Gc have shown protective effects animal models, making them promising vaccine candidates. This study reports recombinant expression, purification, immunogenicity evaluation of sGn sGc produced methylotrophic yeast Pichia pastoris. Initially, was expressed...
Effective drug delivery to the central nervous system (CNS) remains a challenge due blood–brain barrier (BBB). Macromolecules such as proteins and peptides are unable cross BBB have poor therapeutic efficacy little or no distribution. A promising alternative is conjugation of shuttle molecule that can reach CNS via receptor-mediated transcytosis (RMT). Several receptors been described for RMT, low-density lipoprotein receptor-related protein 1 (LRP1). We used phage display technology...