A5058357279- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- HIV Research and Treatment
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- Immune Cell Function and Interaction
- Virology and Viral Diseases
- HIV/AIDS Research and Interventions
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Toxin Mechanisms and Immunotoxins
- HIV-related health complications and treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Vector-borne infectious diseases
- Viral Infections and Immunology Research
- Insect and Pesticide Research
- CAR-T cell therapy research
- vaccines and immunoinformatics approaches
- Viral Infections and Vectors
- SARS-CoV-2 detection and testing
- Transgenic Plants and Applications
- Protein purification and stability
McGill University
2019-2024
Université du Québec à Montréal
2016-2023
University of Manitoba
2021
National Research Council Canada
2021
Université Laval
2021
PROTEO
2021
Université de Montréal
2021
Public Health Agency of Canada
2021
University of Concepción
2014-2015
Centro de Ingeniería Genética y Biotecnología
2004-2014
We report on the development of surface plasmon resonance (SPR) sensors and matching ELISAs for detection nucleocapsid spike antibodies specific to novel coronavirus 2019 (SARS-CoV-2) in human serum, plasma dried blood spots (DBS).
Background: The lungs were historically identified as one of the major anatomic sites for HIV replication in pre-antiretroviral therapy (ART) era. However, their contribution to persistence individuals under suppressive ART remains understudied. Design: We assessed and comprehensively characterized pulmonary mucosal CD4+ T cells HIV-infected (HIV+) receiving long-term versus uninfected participants. Methods: Bronchoalveolar lavage (BAL), bronchial biopsies, matched peripheral blood obtained...
Vaccine design strategies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are focused on the Spike protein or its subunits as main antigen target of neutralizing antibodies. In this work, we propose rapid production methods an extended segment Receptor Binding Domain (RBD) in HEK293SF cells cultured suspension, serum-free media, a major component COVID-19 subunit vaccine under development. The expression RBD, engineered with sortase-recognition motif for protein-based...
The cattle tick, Rhipicephalus (Boophilus) microplus, affects livestock production in many regions of the world. Up to now, widespread use chemical acaricides has led selection acaricide-resistant ticks and environmental contamination. Gavacplus is a subunit vaccine based on recombinant Bm86 tick antigen expressed yeast, capable control infestations R. microplus under controlled conditions. constitutes core element broad programs against this ectoparasite, which acquired immunity combined...
HIV infection promotes the expansion of immunosuppressive regulatory T-cells (Tregs), contributing to immune dysfunction, tissue fibrosis and disease progression. Early antiretroviral treatment (ART) upon improves CD4 count decreases activation. However, Treg dynamics their epigenetic regulation following early ART initiation remain understudied.Treg subsets were characterized by flow cytometry in 103 individuals, including untreated HIV-infected participants acute chronic phases,...
The Bm86 antigen has been used to control ticks of the Boophilus genera in integrated programs that also include use acaricides. Because recent phylogenetic studies have lead inclusion all species within Rhipicephalus genera, we aimed investigate efficacy on biotic potential sanguineus. Domestic dogs were vaccinated with and challenged three instars R. Male female mongrel divided into two groups four animals each, comprising non-vaccinated animals. Immunized given doses an experimental...
Tregs contribute to SIV/HIV disease progression by inhibition of antiviral specific responses and effector T-cell proliferation. also cause tissue fibrosis via transforming growth factor β1 production collagen deposition, which are associated with microbial translocation generalized immune activation. Early ARV initiation upon viral exposure is recommended globally results in improved function recovery reduced persistence. Here, using an acute SIV infection model rhesus macaques, we...
The ongoing COVID-19 pandemic drew global attention to infectious diseases, attracting numerous resources for development of preparedness plans and vaccine platforms-technologies with robust manufacturing processes that can quickly be pivoted target emerging diseases. Newcastle Disease Virus (NDV) has been studied as a viral vector human veterinary vaccines, but its production relies heavily on embryonated chicken eggs, very few studies producing NDV in cell culture. Here, is produced...
Abstract In the era of Biopharma 4.0, process digitalization fundamentally requires accurate and timely monitoring critical parameters (CPPs) quality attributes. Bioreactor systems are equipped with a variety sensors to ensure robustness product quality. However, during biphasic production viral vectors or replication‐competent viruses for gene cell therapies vaccination, current techniques relying on single working sensor can be affected by physiological state change cells due...
Numerous studies in chordates and arthropods currently indicate that Cdx proteins have a major ancestral role the organization of post-head tissues. In urochordate embryos, loss-of-function has notably been shown to impair axial elongation, neural tube (NT) closure pigment cell development. Intriguingly, contrast elongation NT closure, crest (NC)-derived melanocyte/pigment development not reported any other chordate species. To address this, we generated new conditional pan-Cdx functional...
A vaccine candidate to SARS-CoV-2 was constructed by coupling the viral receptor binding domain (RBD) surface of papaya mosaic virus (PapMV) nanoparticle (nano) generate RBD-PapMV vaccine. Immunization mice with coupled enhanced antibody titers and T-cell mediated immune response directed RBD antigen as compared immunization non-coupled formulation (RBD + PapMV nano). Anti-RBD antibodies, generated in vaccinated animals, neutralized infection vitro against ancestral, Delta Omicron variants....
The administration of viral vectored vaccines remains one the most effective ways to respond ongoing novel coronavirus disease 2019 (COVID-19) pandemic. However, pre-existing immunity vector hinders its potency, resulting in a limited choice vectors. Moreover, basic batch mode manufacturing does not allow cost-effectively meet global demand for billions doses per year. To date, exposure humans VSV infection has been limited. Therefore, recombinant vesicular stomatitis virus (rVSV), which...
Recombinant virus-like particles (VLP) antigenically similar to rabbit hemorrhagic disease virus (RHDV) were recently expressed at high levels inside Pichia pastoris cells. Based on the potential of RHDV VLP as platform for diverse vaccination purposes we undertook design, development and scale-up a production process. Conformational stability issues addressed improve process control optimization. Analyses structure, morphology antigenicity these multimers carried out different pH values...
The lungs are relatively unexplored anatomical human immunodeficiency virus (HIV) reservoirs in the antiretroviral therapy (ART) era. Double negative (DN) T cells a subset of that lack expression CD4 and CD8 (CD4- CD8-) may have both regulatory effector functions during HIV infection. Notably, circulating DN were previously described as cellular reservoirs. Here, we undertook thorough analysis pulmonary versus blood people living with (PLWH) under ART. Bronchoalveolar lavage (BAL) fluid...
New influenza strains are constantly emerging, causing seasonal epidemics and raising concerns to the risk of a new global pandemic. Since vaccination is an effective method prevent spread disease reduce its severity, development robust bioprocesses for producing pandemic vaccines exceptionally important. Herein, membrane chromatography-based downstream processing platform with demonstrated industrial application potential was established. Cell culture-derived virus H1N1/A/PR/8/34 harvested...
ABSTRACT Subunit recombinant vaccines against classical swine fever virus (CSFV) are a promising alternative to overcome practical and biosafety issues with inactivated vaccines. One of the strategies in evaluation under field conditions is use new marker E2-based vaccine produced milk adenovirally transduced goats. Previously we had demonstrated efficacy this antigen, which conferred early protection long-lasting immunity CSFV infection. Here, have used simpler downstream process obtain...
Background: Despite the success of antiretroviral therapy (ART) to control viral replication, people living with HIV (PWH) have high levels chronic systemic inflammation and immune dysregulation which drives accelerated co-morbidities including coronary artery disease (CAD). Regulatory T cells (Tregs) ectonucleotidases CD39/CD73 are known be athero-protective via their immunosuppressive anti-inflammatory functions. Design: We assessed dynamics Treg subsets in ART-treated PWH or without CAD...