A5028287780- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Click Chemistry and Applications
- Bacteriophages and microbial interactions
- Peptidase Inhibition and Analysis
- HIV/AIDS drug development and treatment
- Viral Infections and Immunology Research
- RNA regulation and disease
- Viral gastroenteritis research and epidemiology
- Glycosylation and Glycoproteins Research
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- Hepatitis C virus research
- Animal Virus Infections Studies
- Enzyme Catalysis and Immobilization
- Immune responses and vaccinations
- Nuclear Structure and Function
National Research Council Canada
2015-2023
Université de Montréal
1970-2021
Public Health Agency of Canada
2021
McGill University
2021
Université Laval
2021
PROTEO
2021
University of Manitoba
2021
Protein Express (United States)
2021
Boehringer Ingelheim (Canada)
2011
BioPhage Pharma (Canada)
1998-2001
While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about saliva and its relationship systemic levels. Here, we profiled by enzyme-linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor-binding domain (RBD) serum of acute convalescent patients with laboratory-diagnosed COVID-19 ranging from 3-115 days post-symptom onset (PSO), compared negative controls. Anti-SARS-CoV-2 were readily...
Antibody testing against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been instrumental in detecting previous exposures and analyzing vaccine-elicited immune responses. Here, we describe a scalable solution to detect quantify SARS-CoV-2 antibodies, discriminate between natural infection- vaccination-induced responses, assess antibody-mediated inhibition of the spike-angiotensin converting enzyme (ACE2) interaction.
DNA polymerase theta (Polθ) is an attractive synthetic lethal target for drug discovery, predicted to be efficacious against breast and ovarian cancers harboring BRCA-mutant alleles. Here, we describe our hit-to-lead efforts in search of a selective inhibitor human Polθ (encoded by POLQ). A high-throughput screening campaign 350,000 compounds identified 11 micromolar hit, giving rise the N2-substituted fused pyrazolo series, which was validated biophysical methods. Structure-based design...
Abstract While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about mucosal immune and its relationship systemic levels. Since initially replicates upper airway, oral cavity likely an important parameter that influences course of infection, but how it correlates serum not known. Here, we profile by enzyme linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor binding domain (RBD) (n=496)...
We report on the development of surface plasmon resonance (SPR) sensors and matching ELISAs for detection nucleocapsid spike antibodies specific to novel coronavirus 2019 (SARS-CoV-2) in human serum, plasma dried blood spots (DBS).
The huge worldwide demand for vaccines targeting SARS-CoV-2 has necessitated the continued development of novel improved formulations capable reducing burden COVID-19 pandemic. Herein, we evaluated protein subunit vaccine containing a resistin-trimerized spike antigen, SmT1. When combined with sulfated lactosyl archaeol (SLA) archaeosome adjuvant, induced robust antigen-specific humoral and cellular immune responses in mice. Antibodies had strong neutralizing activity, preventing viral...
Nanobodies offer several potential advantages over mAbs for the control of SARS-CoV-2. Their ability to access cryptic epitopes conserved across SARS-CoV-2 variants concern (VoCs) and feasibility engineer modular, multimeric designs, make these antibody fragments ideal candidates developing broad-spectrum therapeutics against current continually emerging VoCs. Here we describe a diverse collection 37 anti-SARS-CoV-2 spike glycoprotein nanobodies extensively characterized as both monovalent...
Using our strongly immunogenic SmT1 SARS-CoV-2 spike antigen platform, we developed antigens based on the Beta & Delta variants of concern (VOC). These elicited higher neutralizing antibody activity to corresponding variant than comparable vaccine formulations original reference strain, while a multivalent generated cross-neutralizing in all three variants. This suggests that current vaccines may be effective at reducing severe disease existing VOC, variant-specific antigens, whether mono-...
Abstract SARS-CoV-2 variants of concern (VOCs) have emerged worldwide, with implications on the spread pandemic. Characterizing cross-reactivity antibodies against these VOCs is necessary to understand humoral response non-hospitalized individuals previously infected SARS-CoV-2, a population that remains understudied. Thirty-two SARS-CoV-2-positive (PCR-confirmed) and Canadian adults were enrolled 14–21 days post-diagnosis in 2020, before emergence B.1.351 (also known as Beta), B.1.617.2...
The HCV p7 protein is not involved in viral RNA replication but essential for production of infectious virus. Based on its putative ion channel activity, belongs to a family proteins known as viroporins that oligomerize after insertion into lipid membrane. To screen compounds capable interfering with function, low-throughput liposome-based fluorescent dye permeability assay was modified and converted robust high-throughput screening assay. Escherichia coli expressing recombinant were grown...
It is now well documented that apoptosis represents the prevalent mode of death in lymphoid cultures and occurs spontaneously late-exponential phase batch following nutrient exhaustion. In an attempt to enhance cell survival these lines, we have initially engineered nonproducing NS/0 myeloma cells with a vector expressing adenoviral E1B-19K protein. transfected were found be more resistant occurring late culture under stressful conditions such as cultivation glutamine-free medium or heat...
Monitoring antibody response to SARS-CoV-2 is critical for assessing the humoral response, especially important considering emergence of multiple variants concern (VOCs).
Early in the COVID-19 pandemic, reagent availability was not uniform, and infrastructure had to be urgently adapted undertake surveillance. Before validation of centralized testing, two enzyme-linked immunosorbent assays (ELISA) were established independently at decentralized sites using different reagents instrumentation. We compared results these assess longitudinal humoral response SARS-CoV-2-positive (i.e., PCR-confirmed), non-hospitalized individuals with mild moderate symptoms, who...
Bispecific antibodies with specificity for tumor antigen and CD3 have been shown to redirect the cytotoxicity of T cells against relevant tumor. Our objective was generate single-chain bispecific (bsSCA) that could retarget mouse cytotoxic lymphocytes (CTL) destroy human ovarian carcinoma in a xenogeneic setting. A bsSCA, 2C11 X B43.13, constructed by genetic engineering expressed mammalian cells. Molecular characteristics, binding properties, ability CTL were studied. Western blot analysis...
Abstract OBJECTIVES Antibody testing against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been instrumental in detecting previous exposures and analyzing vaccine-elicited immune responses. Here, we describe a scalable solution to detect quantify SARS-CoV-2 antibodies, discriminate between natural infection- vaccination-induced responses, assess antibody-mediated inhibition of the spike-angiotensin converting enzyme (ACE2) interaction. METHODS We developed methods reagents...
A nearly full-length cDNA very similar to murine serum amyloid protein was cloned from herpes simplex virus type 2-transformed hamster cells. The expression of mRNA constitutive in these cells and could be superinduced by conditioned medium. Higher rates nuclear runoff transcription transformed induced indicated some regulation at the transcriptional level.
ABSTRACT Recombinant forms of the spike protein SARS-CoV-2 and related viruses have proven difficult to produce with good yields in mammalian cells. Given panoply potential COVID-19 diagnostic tools therapeutic candidates that require purified its importance for ongoing research, we explored new approaches production purification. Three transient gene expression methods based on PEI-mediated transfection CHO or HEK293 cells suspension culture chemically-defined media were compared rapid...
Abstract SARS-CoV-2 subunit vaccines continue to be the focus of intense clinical development worldwide. Protein antigens in these most commonly consist spike ectodomain fused a heterologous trimerization sequence, designed mimic compact, prefusion conformation on virus surface. Since 2020, we have produced dozens such constructs CHO cells, consisting variants with different mutations sequences. This set displayed notable conformational heterogeneity, two distinct trimer species consistently...