A5074960558- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Vaccine Coverage and Hesitancy
- COVID-19 epidemiological studies
- Long-Term Effects of COVID-19
- COVID-19 and Mental Health
- SARS-CoV-2 detection and testing
- COVID-19 Impact on Reproduction
- Animal Virus Infections Studies
- Cancer-related molecular mechanisms research
- COVID-19 and healthcare impacts
- Cardiac, Anesthesia and Surgical Outcomes
- Biomarkers in Disease Mechanisms
- Epigenetics and DNA Methylation
- Heparin-Induced Thrombocytopenia and Thrombosis
- Nuclear Receptors and Signaling
- FOXO transcription factor regulation
- Monoclonal and Polyclonal Antibodies Research
- Computational Drug Discovery Methods
- Ocular Diseases and Behçet’s Syndrome
- vaccines and immunoinformatics approaches
- Hedgehog Signaling Pathway Studies
- Complement system in diseases
- ICT in Developing Communities
- Ultrasound in Clinical Applications
University of Ottawa
2021-2025
Public Health Ontario
2022-2023
University of Calgary
2014-2023
Ottawa Hospital
2022-2023
BC Centre for Disease Control
2023
Manitoba Health
2023
University of Toronto
2022-2023
Toronto Public Health
2023
Cohort (United Kingdom)
2023
Alberta Health
2023
Antibody testing against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been instrumental in detecting previous exposures and analyzing vaccine-elicited immune responses. Here, we describe a scalable solution to detect quantify SARS-CoV-2 antibodies, discriminate between natural infection- vaccination-induced responses, assess antibody-mediated inhibition of the spike-angiotensin converting enzyme (ACE2) interaction.
Patients with cerebral small-vessel disease (CSVD) exhibit perturbed end-artery function and have an increased risk for stroke age-related cognitive decline. Here, we used targeted genome-wide association (GWA) analysis defined a CSVD locus adjacent to the forkhead transcription factor FOXC1. Moreover, determined that linked SNPs influence FOXC1 transcript levels demonstrated patients as young 1 year of age altered CSVD. MRI missense nonsense mutations well FOXC1-encompassing segmental...
Household transmission contributes to SARS-CoV-2 spread, but the role of children in is unclear. We conducted a study that included symptomatic and asymptomatic adults exposed their households with objective determining how transmitted within households.In this case-ascertained antibody-surveillance study, we enrolled Ottawa, Ontario, which at least 1 household member had tested positive for on reverse transcription polymerase chain reaction testing. The enrolment period was September 2020...
Objectives: Many vaccines require higher/additional doses or adjuvants to provide adequate protection for people with HIV (PWH). Our objective was compare COVID-19 vaccine immunogenicity in PWH HIV-negative individuals. Design: In a Canadian multi-center prospective, observational cohort of receiving at least two vaccinations, we measured vaccine-induced immunity 3 and 6 months post 2nd 1-month 3rd doses. Methods: The primary outcome the percentage mounting [co-positivity anti-IgG against...
Recognition of pathogen-or-damage-associated molecular patterns is critical to inflammation. However, most exist within intact microbes/cells and are typically part non-diffusible, stable macromolecules that not optimally immunostimulatory or available for immune detection. Partial digestion following phagocytosis potentially generates new diffusible patterns, however, our current understanding phagosomal biology would have these molecules sequestered destroyed phagolysosomes. Here, we show...
The contributions of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cells to vaccine efficacy and durability are unclear. We investigated relationships between mRNA vaccine-induced spike-specific interferon- gamma (IFN-γ) interleukin-2 (IL-2) T-cell responses neutralizing antibody development in long-term care home staff doubly vaccinated with BNT162b2 or mRNA-1273. impacts pre-existing cross-reactive immunity on cellular humoral vaccination were additionally...
Abstract Clinical outcomes resulting from SARS‐CoV‐2 infection vary widely, ranging asymptomatic cases to the development of mild severe respiratory illness, and in some instances, chronic lingering disease mortality. The underlying biological mechanisms driving this wide spectrum pathogenicity among certain individuals demographics remain elusive. Autoantibodies have emerged as potential contributors severity COVID‐19. Although preliminary reports suggested induction antibodies targeting...
The use of COVID-19 vaccines has been prioritised to protect the most vulnerable-notably, older people. Because fluctuations in vaccine availability, strategies such as delayed second dose and heterologous prime-boost have used. However, effectiveness these frail, people are unknown. We aimed assess antigenicity mRNA-based a real-world setting, with rationed interval dosing 16 weeks between prime boost doses.This prospective observational cohort study was done across 12 long-term care...
Purpose To investigate the robustness and longevity of SARS-CoV-2 immune responses conferred by natural infection vaccination among priority populations such as immunocompromised individuals people with post-acute sequelae COVID-19 in a prospective cohort study (Stop Spread Ottawa—SSO) adults living Ottawa region. In this paper, we describe design, ongoing data collection baseline characteristics participants. Participants Since October 2020, participants who tested positive for...
Background Vaccination against COVID-19 is highly effective in preventing severe disease and hospitalization, but primary COVID mRNA vaccination schedules often differed from those recommended by the manufacturers due to supply chain issues. We investigated impact of delaying second dose on antibody responses mRNA-vaccines a prospective cohort health-care workers Quebec. Methods recruited participants McGill University Health Centre who provided serum or participant-collected dried blood...
Respiratory silicosis is a preventable occupational disease that develops secondary to the aspiration of crystalline silicon dioxide (silica) into lungs, activation NLRP3 inflammasome, and IL-1β production. Cathepsin Z has been associated with development inflammation production; however, mechanism how cathepsin leads production unknown. Here, requirement for in was determined using WT mice deficient Z. The inflammasome macrophages studied Z-deficient bone marrow-derived murine dendritic...
The extent of the COVID-19 pandemic will be better understood through serosurveys and SARS-CoV-2 antibody testing. Dried blood spot (DBS) samples play a central role in large scale serosurveillance by simplifying biological specimen collection transportation, especially Canada. Direct comparative performance data on multiplex assays resulting from identical DBS are currently lacking. In our study, we aimed to provide for BioPlex 2200 IgG (Bio-Rad), V-PLEX Panel 2 (MSD), Elecsys...
Predictors of COVID-19 vaccine immunogenicity and the influence prior severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection require elucidation.Stop Spread Ottawa is a prospective cohort individuals at-risk for or who have been infected with SARS-CoV-2, initially enrolled 10 months beginning October 2020. This was enriched public-facing workers. analysis focuses on safety initial two doses vaccine.Post-vaccination data blood specimens were available 930 participants. 22.8%...
Background: The zebrafish genetic mutant iguana ( igu ) has defects in the ciliary basal body protein Dzip1, causing improper cilia formation. Dzip1 also interacts with downstream transcriptional activators of Hedgehog (Hh), Gli proteins, and Hh signaling is disrupted mutants. governs a wide range developmental processes, including stabilizing developing blood vessels to prevent hemorrhage. Using embryos treated pathway antagonist cyclopamine, we conducted microarray determine genes involved...
<h3>Background:</h3> Early in the COVID-19 pandemic, South Asian community Greater Toronto Area (GTA) was identified as having risk factors for exposure and specific barriers to accessing testing reliable health information, rendering them particularly vulnerable SARS-CoV-2 infection. We sought investigate burden of infection among people GTA, characterize demographic characteristics, perceptions trusted sources information this group. <h3>Methods:</h3> conducted a cross-sectional analysis...
Objectives: Many vaccines require higher/additional doses or adjuvants to provide adequate protection for people living with HIV (PLWH). Here, we compare COVID-19 vaccine-induced antibody neutralization capacity in PLWH vs. HIV-negative individuals following two vaccine doses. Design: In Canadian prospective observational cohorts, inlcuding a multi-centre study of receiving ≥2 vaccinations (mRNA ChAdOx1-S), and parallel controls ( Stop the Spread Ottawa Cohort ), measured 3 months post-dose...
COVID-19 breakthrough infection (BTI) can occur despite vaccination. Using a multi-centre, prospective, observational Canadian cohort of people with HIV (PWH) receiving ≥2 vaccines, we compared the SARS-CoV-2 spike (S) and receptor-binding domain (RBD)-specific IgG levels 3 6 months post second dose, as well 1 month third in PWH without BTI. BTI was defined positivity based on self-report measures (data up to last study visit) or data (up dose 3). The were their symptoms either positive PCR...
Abstract Clinical outcomes resulting from SARS-CoV-2 infection vary widely, ranging asymptomatic cases to the development of mild severe respiratory illness, and in some instances, chronic lingering disease mortality. The underlying biological mechanisms driving this wide spectrum pathogenicity among certain individuals demographics remain elusive. Autoantibodies have emerged as potential contributors severity COVID-19. Although preliminary reports suggested induction antibodies targeting...
Abstract OBJECTIVES Antibody testing against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been instrumental in detecting previous exposures and analyzing vaccine-elicited immune responses. Here, we describe a scalable solution to detect quantify SARS-CoV-2 antibodies, discriminate between natural infection- vaccination-induced responses, assess antibody-mediated inhibition of the spike-angiotensin converting enzyme (ACE2) interaction. METHODS We developed methods reagents...
Abstract Background SARS-CoV-2 infections have disproportionally burdened elderly populations with excessive mortality. While several contributing factors exists, questions remain about the quality and duration of humoral antibody–mediated responses resulting from in unvaccinated individuals. Methods Residual serum/plasma samples were collected individuals undergoing routine polymerase chain reaction testing a community laboratory Canada. The 2020, before vaccines became available. IgG, IgA,...
Abstract Background Vaccination helps prevent SARS-CoV-2 infection and severe COVID-19. However, vaccine-induced humoral immune responses vary among individuals wane over time. We aimed to describe the anti-spike IgG antibody response vaccination identify health demographic factors associated with this children adults. Methods studied a subset of double-vaccinated (n= 151; mean age: 12 ±1.5 years, 46% female) adults 995; 44 ±6.0 60% from Canadian CHILD Cohort. Dried blood spots were...
Abstract Background South Asians represent the largest non-white ethnic group in Canada. The Greater Toronto Area (GTA), home to a high proportion of Asians, emerged as COVID-19 hot spot. Early pandemic, Asian community was identified having risk factors for exposure and specific barriers accessing testing reliable health information, rendering them uniquely vulnerable SARS-CoV-2 infection. Objectives To investigate burden infection among GTA, determine which demographic characteristics were...
This substudy of a prospective case-ascertained household transmission study investigated severe acute respiratory syndrome coronavirus 2 reverse transcription polymerase chain reaction-positive individuals without antibody development and factors associated with nonseroconversion. Approximately 1 8 disease 2019 did not seroconvert. Children, particularly the youngest, were approximately half as likely to seroconvert compared adults. Apart from absence fever/chills, individual symptoms...