A5041105495- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Palliative Care and End-of-Life Issues
- Viral Infections and Outbreaks Research
- Hepatitis Viruses Studies and Epidemiology
- Biosimilars and Bioanalytical Methods
- Childhood Cancer Survivors' Quality of Life
- Bacterial biofilms and quorum sensing
- Grief, Bereavement, and Mental Health
- Library Science and Information Literacy
- ICT in Developing Communities
- Family and Patient Care in Intensive Care Units
- Library Science and Administration
- Vaccine Coverage and Hesitancy
- Cancer and Skin Lesions
- Digital and Traditional Archives Management
- Breast Cancer Treatment Studies
- Ion channel regulation and function
- Myasthenia Gravis and Thymoma
- Breast Lesions and Carcinomas
- Cellular transport and secretion
- Vibrio bacteria research studies
Public Health Agency of Canada
2006-2023
University of Wisconsin–Milwaukee
2015
Greater Poland Cancer Center
2010
Nagoya City University
2006
Health Canada
2005
Canadian Science Centre for Human and Animal Health
2003
Zoological Society of London
1966-1972
Determining the longitudinal molecular evolution of hepatitis B virus (HBV) is difficult due to HBV's genomic complexity and need study paired samples collected over long periods time. In this study, serial were from eight e antigen-negative asymptomatic carriers HBV genotype in 1979 2004, thus providing a 25-year period document long-term HBV. The rate, nature, distribution mutations that emerged 25 years determined by phylogenetic linear regression analysis full-length genome sequences....
Because of the global spread Zika virus, accurate and high-throughput diagnostic immunoassays are needed. We compared sensitivity specificity 5 commercially available virus serologic assays to recommended protocol IgM-capture ELISA plaque-reduction neutralization tests. Most commercial showed low sensitivity, which can be increased.
ABSTRACT Specific genotypes of hepatitis B virus (HBV) are increasingly recognized for their clinical significance and association with particular viral mutations. Although many HBV genotyping methods exist, there has been no standardized or commercially available method direct molecular typing the genome. A newly line probe assay (INNO-LiPA Genotyping assay; Innogenetics N.V., Ghent, Belgium) that allows identification to G was assessed by comparison pre-S1/pre-S2 sequence analysis isolates...
Hepatitis B virus (HBV) genotype G (HBV/G) is an unusual variant, and little known about its epidemiology natural history, particularly the requirement for a co-infecting HBV their relationship during infection. This study investigated quasispecies nature of genotypes in 39 samples collected over 6 year period from 13 HBV/G-infected patients. HBV/G infections were found to occur predominantly males (92 %) primarily associated with male homosexual sex (67 %). All patients infected HBV/A, or...
Viral hepatitis is a major concern worldwide, with A (HAV) and E (HEV) viruses showing sporadic outbreaks while B (HBV) C (HCV) are associated chronic hepatitis, cirrhosis hepatocellular carcinoma. The present study determined the proportion, geographic distribution molecular characterization of among patients seeking medical services at hospitals throughout Kenya. Patients presenting jaundice four selected were recruited (n = 389). Sera tested for presence antibody to through E, HBV surface...
ABSTRACT The nucleotide analog adefovir dipivoxil (ADV) is an effective antiviral treatment for chronic hepatitis B virus (HBV) infection, with resistance to ADV estimated occur less frequently than lamivudine treatment. detection of mutations necessary during therapy monitor and anticipate possible failure. INNO-LiPA HBV DR v2 (LiPA; Innogenetics, Ghent, Belgium) a DNA hybridization line probe assay the polymerase associated ADV. Evaluation this detect was performed by analyzing 38 patients...
Summary. Very little is known of hepatitis B virus (HBV) in Canadian Arctic indigenous populations, where HBV was considered endemic prior to the introduction vaccine. This study expands upon an seroepidemiological survey conducted between 1983 and 1985 throughout Arctic, characterize this population. Archived surface antigen (HBsAg)‐positive sera ( n = 401) were processed for DNA, followed by sequencing phylogenetic analysis HBsAg‐ HBcAg‐coding regions. Sixty‐nine per cent samples (277/401)...
Summary. Lamivudine is effective in suppressing viral replication, normalizing alanine aminotransferase (ALT), and improving histological appearance HBe positive negative hepatitis. It unclear whether hepatitis B virus (HBV) genotype influences the response to lamivudine. We report long‐term of patients with chronic without cirrhosis at baseline treated lamivudine according HBV genotype. Retrospective review charts all monotherapy between 1993 2002. Response therapy defined as ALT normal...
The extent of the COVID-19 pandemic will be better understood through serosurveys and SARS-CoV-2 antibody testing. Dried blood spot (DBS) samples play a central role in large scale serosurveillance by simplifying biological specimen collection transportation, especially Canada. Direct comparative performance data on multiplex assays resulting from identical DBS are currently lacking. In our study, we aimed to provide for BioPlex 2200 IgG (Bio-Rad), V-PLEX Panel 2 (MSD), Elecsys...
<h2>Abstract</h2> Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) belonging to the multilocus sequence type clonal complex 59 (MLST CC59) is predominant community-associated MRSA clone in Asia. This clone, which primarily linked with <i>spa</i> t437, has so far only been reported low numbers among large epidemiological studies Europe. Nevertheless, overall identified some Northern European reference laboratories have increased during past decade. To determine whether <i>S. t437...
The eight genotypes of hepatitis B virus (HBV) exhibit distinct geographical distributions. This study identified HBV and transmission modes associated with acute infection in British Columbia (BC), Canada, from 2001 to 2005. Seventy cases BC were laboratory reports using a standardized case definition. Interviews for risk factors history conducted each case. determined by BLAST comparison analysis the surface (S) or preS gene sequence. To illustrate distribution amongst BC, an annotated map...
We identify two novel mutations in acetylcholine receptor (AChR) causing a slow-channel congenital myasthenia syndrome (CMS) three unrelated patients (Pts). Pt 1 harbors heterozygous βV266A mutation (p.Val289Ala) the second transmembrane domain (M2) of AChR β subunit (CHRNB1). Pts 2 and 3 carry same at an equivalent site ε (CHRNE), εV265A (p.Val285Ala). The mutant residues are conserved across all subunits species components valine ring channel pore, which is positioned four above leucine...
Objective The prevalence and distribution of hepatitis B virus (HBV) genotypes in Canada is not known. Genotypic analysis may contribute to a better understanding HBV strain transmission risk. Methods surface antigen (HBsAg) positive samples acute (n = 152) chronic 1533) submitted for or reference genotype testing between 2006 2012 were analyzed. HBsAg coding region was amplified determine the by INNO-LiPA assay sequence analysis. Single multivariate analyses used describe genotypes'...
COVID-19 seroprevalence studies use serum/plasma samples to detect SARS-CoV-2 IgG. Data supporting alternate specimen types and freeze-thaw antibody stability is lacking. The of IgG other immunoglobulins in multiple blood sample stored differing conditions cycles (FTCs) was evaluated.Serum, plasma, heparinized-plasma were collected from recovered individuals. Samples underwent testing for antibodies upon collection, after each 10-12 FTCs, storage at -70°C, -20°C, 4°C, room-temperature days...
Summary Recent studies suggest that withdrawal of hepatitis B immune globulin ( HBIG ) and nucleos(t)ide analogues NA prophylaxis may be considered in HBV surface antigen HB sAg)‐negative liver transplant LT recipients with a low risk disease recurrence. However, the frequency occult infection OBI variants after current era potent therapy is unknown. Twelve on were tested matched plasma peripheral blood mononuclear cells PBMCs for quasispecies by in‐house nested PCR next‐generation...
Hepatitis B virus (HBV) vaccination starting at birth is approximately 95% effective in preventing mother-to-child transmission to infants born HBV-infected mothers. A higher risk of associated with a highly viremic mother, often due transplacental exposure, while later horizontal much less common, particularly following complete vaccination. This study reports case infection an older child despite appropriate immunoprophylaxis and apparent protective immune response post-vaccination. Two...
Serological assays designed to detect SARS-CoV-2 antibodies are being used in serological surveys and other specialized applications. As a result, ensure that the outcomes of testing meet high quality standards, evaluations required assess performance these proficiency laboratories performing them.A panel 60 plasma/serum samples from blood donors who had reverse transcriptase-polymerase chain reaction (RT-PCR) confirmed infections 21 negative were secured distributed interested within Canada...
In chronic hepatitis B (CHB), D virus (HDV) superinfection can lead to acute liver failure. The incidence of HDV is unknown, but often detected in immigrants from endemic countries. this report, we characterize long-term clinical and virological outcomes a (HBV) infected carrier before after superinfection, acquired their spouse having HBV/HDV co-infection. A 38 year-old Mongolian male with CHB on anti-HBV therapy developed failure following superinfection. Although he recovered, avoiding...
Abstract Background and Objectives Hepatitis B virus (HBV) genotypes (A–H) have a distinct geographic distribution are highly associated with the country of birth. Canada has experienced increased immigration over past decade, primarily from regions where HBV is endemic. This study investigated proportions trends within blood donor clinical populations period 2016–2021. Materials Methods Study samples involved two cohorts: (1) Canadian donors ( n = 246) deferred donation due to test...
Over 296 million people worldwide are living with chronic Hepatitis B (CHB) infection who require monitoring of viral activity and disease progression. Serum HBV RNA is a promising new biomarker in CHB management. No standardized method for serum quantification has been established. To develop validate, both analytically clinically, 3′ RACE RT-qPCR assay RNA. The was developed using published primers. analytical limit detection quantification, linearity, inter- intra-assay repeatability,...