A5085317996- Cardiac Ischemia and Reperfusion
- Acute Kidney Injury Research
- Immune Response and Inflammation
- Cardiac electrophysiology and arrhythmias
- Pancreatic function and diabetes
- Cardiovascular Function and Risk Factors
- COVID-19 Clinical Research Studies
- Mesenchymal stem cell research
- SARS-CoV-2 and COVID-19 Research
- Tissue Engineering and Regenerative Medicine
- Complement system in diseases
- Renin-Angiotensin System Studies
- Signaling Pathways in Disease
- Diabetes Treatment and Management
- Chronic Kidney Disease and Diabetes
- Receptor Mechanisms and Signaling
- Cardiac Fibrosis and Remodeling
- Cardiomyopathy and Myosin Studies
- Sphingolipid Metabolism and Signaling
- Cardiac Arrest and Resuscitation
- Galectins and Cancer Biology
- Electrolyte and hormonal disorders
- Heart Failure Treatment and Management
- Cell death mechanisms and regulation
- MRI in cancer diagnosis
University of Ottawa
2019-2025
Ottawa Hospital
2020-2024
Ottawa Hospital Research Institute
2023
Universidade Federal do ABC
2011-2019
We investigated whether the pathways linked to Toll-like receptors 2 and 4 (TLRs) are involved in renal ischemia-reperfusion (I/R)-induced cardiac hypertrophy. Wild type (WT) C57BL/6J, TLR2-/- TLR4-/- mice were subjected left kidney ischemia for 60 min followed by reperfusion 5, 8, 12 15 days. Proton density magnetic resonance showed alterations injured from WT mice, together with signs of parenchymal edema higher levels vimentin mRNA, accompanied by: (i) small, but significant, increase...
Abstract Clinical outcomes resulting from SARS‐CoV‐2 infection vary widely, ranging asymptomatic cases to the development of mild severe respiratory illness, and in some instances, chronic lingering disease mortality. The underlying biological mechanisms driving this wide spectrum pathogenicity among certain individuals demographics remain elusive. Autoantibodies have emerged as potential contributors severity COVID‐19. Although preliminary reports suggested induction antibodies targeting...
Background/Aims: To investigate the role of sympathetic nervous system (SNS) and reninangiotensin (RAS) in renal ischemia/reperfusion-induced (I/R) cardiac inflammatory profile.Methods: Left kidney ischemia was induced male C57BL/6 mice for 60 min, followed by reperfusion 12 days, treatment with or without atenolol, losartan, enalapril.The expression vimentin atrial natriuretic factor (ANF) heart has been investigated RT-PCR.In tissue, levels β 1 -adrenoreceptors, adenylyl cyclase, cyclic...
The objectives of this study were to evaluate the effect low-level laser irradiation (LLLI) on bovine oocyte and granulosa cells metabolism during in vitro maturation (IVM) further embryo development. Cumulus-oocytes complexes (COCs) subjected (experimental group) or not (control with LLLI a 633-nm wavelength 1 J/cm 2 fluency. COCs evaluated after 30 min, 8, 16, 24 h IVM. Cumulus for cell cycle status, mitochondrial activity, viability (flow cytometry). Oocytes assessed meiotic progression...
The sarcolemmal membrane associated proteins (SLMAPs) belong to the super family of tail anchored which serve diverse roles in biology including cell growth, protein trafficking and ion channel regulation. Mutations human SLMAP have been linked Brugada syndrome with putative deficits sodium (Nav1.5) resulting aberrant electrical activity heart function. Three main isoforms (SLMAP1 (35 kDa), SLMAP2 (45 SLMAP3 (91 kDa)) are expressed myocardium but their precise role remains be defined. Here...
Inflammation plays an important role in the development of cardiovascular diseases (CVDs), suggesting that immune system is a target therapeutic interventions used for treating CVDs. This study evaluated mechanisms underlying inflammatory response and cardiomyocyte hypertrophy associated with bacterial lipopolysaccharide (LPS)- or heat shock protein 60 (HSP60)-induced Toll-like receptor (TLR) stimulation effect small interfering RNA (siRNA) against Ca2+/calmodulin-dependent kinase II delta B...
Renal ischemia/reperfusion induces a systemic inflammatory response that is directly related to the development of cardiac hypertrophy due cardiorenal syndrome type 3. Classic pathways have been extensively investigated in cardiovascular diseases, including participation inflammasome caspase-1-dependent IL-1β cleavage.In this study, we aimed understand how lack caspase-1 would impact hypertrophic and apoptotic heart after renal ischemia/reperfusion.Wildtype knockout animals were submitted...
Abstract Clinical outcomes resulting from SARS-CoV-2 infection vary widely, ranging asymptomatic cases to the development of mild severe respiratory illness, and in some instances, chronic lingering disease mortality. The underlying biological mechanisms driving this wide spectrum pathogenicity among certain individuals demographics remain elusive. Autoantibodies have emerged as potential contributors severity COVID-19. Although preliminary reports suggested induction antibodies targeting...
Tissue remodeling is usually dependent on the deposition of extracellular matrix that may result in tissue stiffness and impaired myocardium contraction.We had previously demonstrated renal ischemia/reperfusion (I/R) able to induce development cardiac hypertrophy mice. Therefore, we aimed characterize I/R-induced hypertrophy.C57BL/6 J mice were subjected 60 minutes' unilateral pedicle occlusion, followed by reperfusion for 5, 8, 12 or 15 days. Gene expression, protein abundance morphometric...
Chronic kidney disease (CKD) is a worldwide health burden with increases risk of end-stage renal function if left untreated. CKD induced in the context metabolic syndrome (MS) risks hypertension, hyperglycemia, excess body fat and dyslipidemia. To test combining high-fat diet (HFD) regimen onto hypertensive/ diabetic phenotype would mimic features MS induced-CKD mice, hyperglycemia was genetically hypertensive mice (Lin), followed by HFD regimen. For that, 8-week-old male were subjected to...
Canagliflozin (CANA) is a sodium-glucose cotransporter 2 (SGLT2) inhibitor with blood glucose lowering effects. CANA also promotes kidney protection in patients cardiovascular diseases and type diabetes (T2D), as well normoglycemic hypertension or heart failure. Clinical studies, although conduct both sexes, do not report sex-dependent differences T2DM treated CANA. However, the impact of 1 diabetes, outcomes such cohort needs further understanding. To analyze effects mice combined was...
Background It is known that renal ischemia reperfusion (I/R) generates a systemic inflammatory state, promoting molecular and morphological changes in the heart tissue, but mechanisms remain unknown. TLRs are largely studied to participate of process, moreover it has been demonstrated their role cardiac hypertrophy (CH) development. Aim The aim this study was investigate whether pathways involved I/R‐induced CH. Methods/Results C57BL/6J, TLR2‐/‐ TLR4‐/‐ mice were subjected unilateral 60 min...
The sarcolemmal membrane-associated proteins (SLMAPs) are a family of tail-anchored membrane generated by alternative splicing the SLMAP gene. A ubiquitously expressed isoform 3 encompasses an N-terminal FHA domain with extended coiled-coil structure and has been implicated in cell cycle control. Heart function transgenic mice cardiac-specific overexpression SLMAP3 cDNA driven α myosin heavy chain promoter was evaluated echocardiography. qPCR western blot were used to analyze gene protein...
Angiotensin-converting enzyme 2 (ACE2) hydrolyzes angiotensin (Ang) II to Ang-(1-7), promoting vasodilatation, and inhibiting oxidative stress inflammation. Plasma membrane ACE2 is the receptor for all known SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) viral variants. In COVID-19 infection, soluble variants may act as decoys bind neutralize coronavirus, reducing its tissue infectivity. Furthermore, have been proposed potential therapeutics kidney disease hypertensive...
Background The immune system consists of an array cells and molecules distributed throughout the organism; its function is to recognize antigens or molecular structures, generating effective response in pathogens destruction inactivation. It can also act via complement (CS). cardiac tissue a main target several cardiovascular diseases (CVD) are preceded by general local inflammation, contributing directly remodeling changes heart tropism. known that renal ischemia/reperfusion (I/R) may...
Cardiac complications are one of the main causes death in diabetes. Several studies have shown anti-diabetic effects bone marrow mesenchymal stromal cells (MSC). In present study, we investigated whether MSC-transplantation can revert cardiac dysfunction streptozotocin-induced diabetic rats and immunoregulatory MSC were examined. The divided three groups: Non-diabetic, Diabetic Diabetic-Treated that received 5x106 4 weeks after establishment Four MSC-therapy, systemic metabolic parameters,...