A5080886195- Tissue Engineering and Regenerative Medicine
- Muscle metabolism and nutrition
- Cardiac electrophysiology and arrhythmias
- Nuclear Structure and Function
- Cardiac Ischemia and Reperfusion
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Hormonal and reproductive studies
- Mesenchymal stem cell research
- Genetics and Physical Performance
- CRISPR and Genetic Engineering
- Metabolomics and Mass Spectrometry Studies
- Congenital heart defects research
- Stress Responses and Cortisol
- Acute Kidney Injury Research
- Electrospun Nanofibers in Biomedical Applications
- Sports Performance and Training
- Immune Response and Inflammation
- Ion channel regulation and function
- Pancreatic function and diabetes
- Histone Deacetylase Inhibitors Research
- Cardiovascular Function and Risk Factors
- MicroRNA in disease regulation
- Ginseng Biological Effects and Applications
Universidade Federal do Rio de Janeiro
2016-2025
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2015-2025
National Institute of Science and Technology in Regenerative Medicine
2023
University Center Augusto Motta
2019-2020
Instituto Nacional de Cardiologia
2020
National Institute of Science and Technology for Structural Biology and Bioimaging
2014-2019
Hospital Dr. Gustavo Fricke
2013-2018
Life & Brain (Germany)
2015
University of Bonn
2015
Abstract Diabetes mellitus (DM) encompasses a multitude of secondary disorders, including heart disease. One the most frequent and potentially life threatening disorders DM-induced disease is ventricular tachycardia (VT). Here we show that toll-like receptor 2 (TLR2) NLRP3 inflammasome activation in cardiac macrophages mediate production IL-1β DM mice. causes prolongation action potential duration, induces decrease potassium current an increase calcium sparks cardiomyocytes, which are...
We investigated whether the pathways linked to Toll-like receptors 2 and 4 (TLRs) are involved in renal ischemia-reperfusion (I/R)-induced cardiac hypertrophy. Wild type (WT) C57BL/6J, TLR2-/- TLR4-/- mice were subjected left kidney ischemia for 60 min followed by reperfusion 5, 8, 12 15 days. Proton density magnetic resonance showed alterations injured from WT mice, together with signs of parenchymal edema higher levels vimentin mRNA, accompanied by: (i) small, but significant, increase...
Direct analysis of isolated mitochondria from old mice enables a better understanding heart senescence dysfunction. Despite well-defined senescent phenotype in cardiomyocytes, the mitochondrial state aged cardiomyocytes is still unclear. Here, we report data about function mice. Isolated cardiomyocytes’ were obtained by differential centrifugation and young hearts to perform functional analyses O2 consumption, transmembrane potential, ROS formation, ATP production, swelling. Our results show...
Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex matrix. However, evaluating extent of cardiomyocyte repopulation decellularized after recellularization attempts is challenging. Here, we describe a unique combination biochemical, biomechanical, histological, physiological parameters for quantifying efficiency tissue-engineered patches compared native tissue. Human embryonic stem...
Background Several studies have correlated protein restriction associated with other nutritional deficiencies the development of cardiovascular and renal diseases. The driving hypothesis for this study was that Ang II signaling pathways in heart kidney are affected by chronic protein, mineral vitamin restriction. Methodology/Principal Findings Wistar rats aged 90 days were fed from weaning either a control or deficient diet mimics those used impoverished regions worldwide. Such...
Abstract Patient-specific cardiomyocytes obtained from induced pluripotent stem cells (CM-iPSC) offer unprecedented mechanistic insights in the study of inherited cardiac diseases. The objective this work was to a type 2 long QT syndrome (LQTS2)-associated mutation (c.1600C > T KCNH2, p.R534C hERG) CM-iPSC. Peripheral blood mononuclear were isolated two patients with R534C and iPSCs generated. In addition, same inserted control iPSC line by genome editing using CRISPR/Cas9. Cells...
Athletes use hypoxic training methods to enhance their performance under altitude conditions. Comparative studies involving populations from low (500–2,000 m) and moderate (2,000–3,000 altitudes offer an opportunity understand the mechanisms behind adaptations hypoxia. The present study combined data metabolomics analysis based on gas- liquid-chromatography mass spectrometry (GC-MS LC-MS) compare plasma profiles 80 adolescent athletes at moderate- or altitudes. 161 metabolites were...
Diabetic patients present a high level of cardiac arrhythmias and risk sudden death. The renin–angiotensin system (RAS) plays key role in diabetes diseases. study aimed to evaluate whether an angiotensin-converting enzyme 2 (ACE2) activator, diminazene aceturate (DIZE), could improve the streptozotocin (STZ)-induced electrical changes ventricular repolarization hyperglycaemic rats. Hyperglycaemia was induced Wistar male rats with STZ (60 mg/kg/iv). After 4 weeks injection, were daily treated...
High cardiorespiratory capacity is a key determinant of human performance and life expectancy; however, the underlying mechanisms are not fully understood. The objective this pilot study was to investigate biochemical signatures endurance-performance athletes using high-resolution nontargeted metabolomics.Elite long-distance runners with similar training anthropometrical records were studied. After athletes' maximal oxygen consumption (V˙O2max) measured, they divided into 2 groups: low...
Humoral factors released during ischemic preconditioning (IPC) protect the myocardium against Ischemia/Reperfusion (I/R) injury. We have recently identified 10kDa-heat shock protein (HSP10) and a fraction of small 5-10kDa peptides (5-10sP) in coronary effluent IPC-treated hearts demonstrated their cardioprotective potential. here used our isolated mitochondria model to characterize impact exogenous HSP10 5-10sP on function from subjected I/R Isolated perfused rat were submitted 30-min global...
Doxorubicin (Dox) is a chemotherapy drug with limited application due to cardiotoxicity that may progress heart failure. This study aims evaluate the role of cardiomyocytes derived from mouse embryonic stem cells (CM-mESCs) in treatment Dox-induced cardiomyopathy (DIC) mice. The cell (mESC) line E14TG2A was characterized by karyotype analysis, gene expression using RT-PCR and immunofluorescence. Cells were transduced luciferase 2 submitted cardiac differentiation. Total conditioned medium...
Decellularized extracellular matrix (dECM) from human atria preserves key native components that directed the cardiac differentiation of hiPSCs to an atrial-like phenotype, yielding a twofold increase functional cells.
Diabetes mellitus is a severe chronic disease leading to systemic complications, including cardiovascular dysfunction. Previous cell therapy studies have obtained promising results with the use bone marrow mesenchymal stromal cells derived from healthy animals (MSCc) in diabetes animal models. However, ability of MSC diabetic rats improve functional cardiac parameters still unknown.To investigate whether bone-marrow-derived (MSCd) would contribute recover metabolic and electrical properties...
Rationale Central questions such as cardiomyocyte subtype emergence during cardiogenesis or the availability of subtypes for cell replacement therapy require selective identification and purification atrial ventricular cardiomyocytes. However, current methodologies do not allow a transgene-free isolation cardiomyocytes due to lack specific surface markers. Methods Results In order develop marker-based procedures subtypes, we performed an antibody-based screening on embryonic mouse hearts....
During fetal development, cardiomyocytes switch from glycolysis to oxidative metabolism sustain the energy requirements of functional cells. State-of-the-art cardiac differentiation protocols yield phenotypically immature cardiomyocytes, and common methods improve metabolic maturation require multistep induce only after specification is completed. Here, we describe a method using ventricle-derived decellularized extracellular matrix (dECM) that promoted early-stage differentiated human...
Testosterone is a hormone that plays key role in carbohydrate, fat, and protein metabolism. deficiency associated with multiple comorbidities, e.g., metabolic syndrome type 2 diabetes. Despite its importance many pathways, the mechanisms by which it controls metabolism are not fully understood. The present study investigated short-term changes of pharmacologically induced castration and, subsequently, testosterone supplementation healthy young males. Thirty subjects were submitted to...