A5079460105- Protein Kinase Regulation and GTPase Signaling
- Cancer-related Molecular Pathways
- Enzyme Structure and Function
- PI3K/AKT/mTOR signaling in cancer
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Cell death mechanisms and regulation
- Biochemical and Molecular Research
- Cancer, Hypoxia, and Metabolism
- Plant biochemistry and biosynthesis
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Mesenchymal stem cell research
- Endoplasmic Reticulum Stress and Disease
- CRISPR and Genetic Engineering
- Pancreatic function and diabetes
- Chronic Lymphocytic Leukemia Research
- RNA Research and Splicing
- Synthesis and biological activity
- HER2/EGFR in Cancer Research
- Biofuel production and bioconversion
- RNA Interference and Gene Delivery
- NF-κB Signaling Pathways
- 14-3-3 protein interactions
- Drug Transport and Resistance Mechanisms
University of Southern Denmark
2014-2024
Universidade Federal do Rio de Janeiro
2013-2017
Iberdrola (Spain)
2017
University of Chile
2016
National Institute of Science and Technology for Structural Biology and Bioimaging
2015
Universidade Federal da Bahia
2012
Hospital Ana Nery
2012
University of California, San Diego
2003
University of Cologne
2001
Norwegian Institute of Bioeconomy Research
1993-2000
A systematic analysis reveals that out of 20 protein kinases examined, specific for either Ser/Thr or Tyr, the majority are extremely sensitive to staurosporine, with IC 50 values in low nanomolar range. few them however, notably CK1 and CK2, mitogen‐activated (MAP) kinase protein‐tyrosine CSK, relatively refractory staurosporine inhibition, exhibiting micromolar With all tested, namely PKA, CK1, MAP (ERK‐1), c‐Fgr, Lyn, CSK TPK‐IIB/p38 syk , inhibition was competitive respect ATP,...
Oral transmission of Trypanosoma cruzi, the causative agent Chagas disease, is most important route infection in Brazilian Amazon and Venezuela. Other South American countries have also reported outbreaks associated with food consumption. A recent study showed importance parasite contact oral cavity to induce a highly severe acute disease mice. However, it remains uncertain primary site entry multiplication due an infection. Here, we evaluated presence T. cruzi Dm28c luciferase (Dm28c-luc)...
The highest CK2 activity was found in mouse testicles and brain, followed by spleen, liver, lung, kidney heart. values were directly correlated with the protein expression level of subunits α (catalytic) β (regulatory). α′ subunit only detected brain testicles. By contrast, Northern blot analyses CK2α mRNA revealed a somewhat different picture. Here, strongest signals obtained for heart lung. In kidney, spleen mRNAs weakly detectable. For CK2α′ distribution strong observed liver case CK2β...
Glioblastoma multiforme is the most common primary brain tumor and one of aggressive types cancer in adults. Survival signaling apoptosis resistance are hallmarks malignant glioma cells. However, recent studies have shown that other cell death such as autophagy can be induced This suggests stimulation this process may explored new therapeutic strategies against glioblastoma multiforme. Protein kinase CK2 a highly conserved constitutively active enzyme promotes numerous cellular processes...
The DNA-dependent protein kinase (DNA-PK) is a nuclear complex composed of large catalytic subunit (DNA-PKcs) and heterodimeric DNA-targeting Ku. DNA-PK major component the non-homologous end-joining (NHEJ) repair mechanism, which activated in presence DNA double-strand breaks induced by ionizing radiation, reactive oxygen species radiomimetic drugs. We have recently reported that down-regulation CK2 siRNA interference results enhanced cell death specifically DNA-PKcs-proficient human...
Background Here we report the monitoring of digestive tract colonization Rhodnius prolixus by Trypanosoma cruzi using an accurate determination parasite load qPCR coupled with fluorescence and bioluminescence imaging (BLI). These complementary methods revealed critical steps necessary for population to colonize insect gut establish vector infection. Methodology/Principal Findings analysis in showed several limitations due mainly presence digestive-derived products that are thought degrade...
Tamoxifen resistance is a major clinical problem in the treatment of estrogen receptor alpha-positive breast tumors. It is, at present, unclear what exactly causes tamoxifen resistance. For decades, chlorpromazine has been used for treating psychotic diseases, such as schizophrenia. However, compound now also recognized multitargeting drug with diverse potential applications, example, it antiproliferative properties and can reverse toward antibiotics bacteria. Furthermore, multidrug caused...
Abstract Background Pancreatic cancer is a complex genetic disorder that characterized by rapid progression, invasiveness, resistance to treatment and high molecular heterogeneity. Various agents have been used in clinical trials showing only modest improvements with respect gemcitabine-based chemotherapy, which continues be the standard first-line for this disease. However, owing overwhelming alterations reported pancreatic cancer, there increasing focus on targeting pathways networks,...
Protein kinase CK2 is a highly conserved serine/threonine ubiquitously distributed in all investigated eukaryotes and involved several intracellular pathways which control, among others, cell cycle, proliferation, apoptosis transformation. Similarly, the PI3K-AKT pathway deregulation has been shown to play an important role tumor development response anti-cancer treatment. In this study, it that specific inhibition of signaling combination with depletion subunits by antisense...
CK2 is a ubiquitous serine/threonine protein kinase, which has the potential to catalyze generation of large proportion human phosphoproteome. Due its role in numerous cellular functions and general anti-apoptotic activity, an important target research with therapeutic potential. This emphasizes need for cell-permeable highly potent selective inhibitors photoluminescence probes investigating phosphorylation networks living cells. Previously, we had developed bisubstrate (CK2-targeted ARCs)...