A5089224719- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Hormonal and reproductive studies
- Muscle metabolism and nutrition
- Melanoma and MAPK Pathways
- SARS-CoV-2 and COVID-19 Research
- Histone Deacetylase Inhibitors Research
- Stress Responses and Cortisol
- COVID-19 epidemiological studies
- Ferroptosis and cancer prognosis
- vaccines and immunoinformatics approaches
- CAR-T cell therapy research
- Click Chemistry and Applications
- Prostate Cancer Treatment and Research
- Immune responses and vaccinations
- Mosquito-borne diseases and control
- Synthesis and biological activity
- Antimicrobial agents and applications
- Ubiquitin and proteasome pathways
- Tryptophan and brain disorders
- COVID-19 Clinical Research Studies
- Organometallic Compounds Synthesis and Characterization
- Advanced Proteomics Techniques and Applications
- Cancer, Lipids, and Metabolism
- Pharmacological Effects and Assays
Lund University
2023-2025
Universidade do Estado do Rio de Janeiro
2025
Universidade Federal do Rio de Janeiro
2021-2024
Skåne University Hospital
2024
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2022
Universidade Federal de Alagoas
2017
Background: Prostate cancer therapy with surgical or chemical castration GnRH agonists has been linked to elevated FSH levels, which may contribute secondary health disorders, including atherosclerosis and diabetes. Although recent findings suggest a role for beyond the reproductive system, its metabolic impact remains unclear difficult disentangle from that of androgens. In this study, we examined changes induced by distinguished them those caused testosterone. Methods: Plasma samples...
Using several melanoma proteomics data sets we created a single analysis platform that enables the discovery, knowledge build, and validation of diagnostic, predictive, prognostic biomarkers at protein level. Quantitative mass-spectrometry-based proteomic was obtained from five independent cohorts, including 489 tissue samples 394 patients with accompanying clinical metadata. We established an interactive R-based web comparison levels across diverse supports correlation between proteins...
The Zika virus (ZIKV) can be vertically transmitted, causing congenital syndrome (CZS) in fetuses. ZIKV infection early gestational trimesters increases the chances of developing CZS. This involves several pathologies with a complex diagnosis. In this work, we aim to identify biological processes and molecular pathways related CZS propose series putative protein metabolite biomarkers for prognosis pregnancy trimesters. We analyzed serum samples healthy pregnant women ZIKV-infected bearing...
The utilization of PD1 and CTLA4 inhibitors has revolutionized the treatment malignant melanoma (MM). However, resistance to targeted immune-checkpoint-based therapies still poses a significant problem.
Abstract Background Although older adults are at a high risk of severe or critical Covid-19, there many cases unvaccinated centenarians who had silent infection recovered from mild moderate Covid-19. We studied three Brazilian supercentenarians, than 110 years, survived Covid-19 in 2020 before being vaccinated. Results Despite their advanced age, humoral immune response analysis showed that these individuals displayed robust levels IgG and neutralizing antibodies (NAbs) against SARS-CoV-2....
The severe acute respiratory syndrome spread worldwide, causing a pandemic. SARS-CoV-2 mutations have arisen in the spike, glycoprotein at viral envelope and an antigenic candidate for vaccines against COVID-19. Here, we present comparative data of glycosylated full-length ancestral D614G spike together with three other transmissible strains classified by World Health Organization as variants concern: beta, gamma, delta. By showing that has less hydrophobic surface exposure trimer...
Introduction While Immune checkpoint inhibition (ICI) therapy shows significant efficacy in metastatic melanoma, only about 50% respond, lacking reliable predictive methods. We introduce a panel of six proteins aimed at predicting response to ICI therapy. Methods Evaluating previously reported two untreated melanoma cohorts, we used published model (EaSIeR score) identify potential distinguishing responders and non-responders. Results Six initially identified the cohort correlated with...
Testosterone is a hormone that plays key role in carbohydrate, fat, and protein metabolism. deficiency associated with multiple comorbidities, e.g., metabolic syndrome type 2 diabetes. Despite its importance many pathways, the mechanisms by which it controls metabolism are not fully understood. The present study investigated short-term changes of pharmacologically induced castration and, subsequently, testosterone supplementation healthy young males. Thirty subjects were submitted to...
Sulfonylhydrazones are privileged structures with multifaceted pharmacological activity. Exploring the hypoglycemic properties of these organic compounds, we previously revealed a new series N-sulfonylhydrazones (NSH) as antidiabetic drug candidates. Here, evaluated microsomal metabolism, chemical stability, and permeability profile NSH prototypes, focusing on pharmacokinetic differences in N-methylated non-N-methylated analogs. Our results demonstrated that analogs (LASSBio-1772...
Abstract The utilization of PD1 and CTLA4 inhibitors has revolutionized the treatment malignant melanoma (MM). However, resistance to targeted immune-checkpoint-based therapies still poses a significant problem. Here we mine large scale MM proteogenomic data integrating it with cell line dependency screen, drug sensitivity identify druggable targets forecast efficacy resistance. Leveraging protein profiles from established subtypes molecular structures 82 cancer drugs, identified nine...
Abstract While Immune checkpoint inhibition (ICI) therapy shows significant efficacy in metastatic melanoma, only about 50% respond, lacking reliable predictive methods. We introduce a panel of six proteins aimed at predicting response to ICI therapy. Evaluating previously reported two untreated melanoma cohorts, we used published model (EaSIeR score) identify potential distinguishing responders and non-responders. Six initially identified the cohort correlated with predicted cohort....
Long term effect of testosterone (T) deficiency impairs metabolism and is associated with muscle degradation metabolic disease. The association seems to have a bidirectional nature not well understood. present study aims investigate the early unidirectional induced T changes by measuring fasting amino acid (AA) levels in human model, which short-term alterations were induced. We designed model 30 healthy young males pharmacologically changes, resulted three time points for blood collection:...
Summary The dynamics of more than 1900 mitochondrial proteins was explored through quantitative proteomics in 151 melanoma-related tissue samples both surgical and autopsy origin. Dysregulation pathways primary tumors, metastases, peritumoral tissues correlated with age survival patients, as well tumor cell proliferation the BRAF mutation status tumors. outlined proteomic landscape confirmed central role a pathologically upregulated translation machinery oxidative phosphorylation (OXPHOS)...
Summary Malignant melanoma (MM) develops from the melanocytes and in its advanced stage is most aggressive type of skin cancer. Here we report a comprehensive analysis on prospective cohort study, including non-tumor, primary metastasis tissues (n=77) with corresponding plasma samples (n=56) patients malignant melanoma. The tumors surrounding were characterized combination high-throughput analyses quantitative proteomics, phosphoproteomics, acetylomics, whole exome sequencing (WES) combined...
LASSBio-1920 was synthesized due to the poor solubility of its natural precursor, combretastatin A4 (CA4). The cytotoxic potential compound against human colorectal cancer cells (HCT-116) and non-small cell lung (PC-9) evaluated, yielding IC50 values 0.06 0.07 μM, respectively. Its mechanism action analyzed by microscopy flow cytometry, where found induce apoptosis. Molecular docking simulations enzymatic inhibition study with wild-type (wt) EGFR indicated enzyme-substrate interactions...
ABSTRACT Testosterone is a hormone that plays key role in carbohydrate, fat, and protein metabolism. deficiency associated with multiple comorbidities, e.g ., metabolic syndrome type 2 diabetes. Despite its importance many pathways, the mechanisms by which it controls metabolism are not fully understood. The present study investigated short-term changes of pharmacologically induced castration testosterone supplementation healthy young males. Thirty subjects were submitted to depletion (TD)...
Abstract The severe acute respiratory syndrome CoV-2 rapidly spread worldwide, causing a pandemic. After period of evolutionary stasis, set SARS-CoV-2 mutations has arisen in the spike, leading glycoprotein at viral envelope and primary antigenic candidate for vaccines against 2019 CoV disease (COVID-19). Here, we present comparative biochemical data glycosylated full-length ancestral D614G spike together with three other highly transmissible strains classified by World Health Organization...