A5033497190- Melanoma and MAPK Pathways
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Biotin and Related Studies
- Ubiquitin and proteasome pathways
- Coenzyme Q10 studies and effects
- Catalytic Cross-Coupling Reactions
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Histone Deacetylase Inhibitors Research
- Organoboron and organosilicon chemistry
- Chemical synthesis and alkaloids
- Advanced Proteomics Techniques and Applications
- Plant biochemistry and biosynthesis
- Ferroptosis and cancer prognosis
- Sulfur-Based Synthesis Techniques
- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
- COVID-19 Clinical Research Studies
- Natural product bioactivities and synthesis
- SARS-CoV-2 and COVID-19 Research
- Cancer, Hypoxia, and Metabolism
- Immunodeficiency and Autoimmune Disorders
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
Lund University
2021-2025
Universidade Federal do Rio de Janeiro
2014-2024
Universidade Federal do Ceará
2020
Abstract The MM500 meta‐study aims to establish a knowledge basis of the tumor proteome serve as complement genome and transcriptome studies. Somatic mutations their effect on have been extensively characterized in melanoma. However, effects these genetic changes proteomic landscape impact cellular processes melanoma remain poorly understood. In this study, quantitative mass‐spectrometry‐based analysis is interfaced with pathological characterization, associated clinical data. landscape,...
Abstract The MM500 study is an initiative to map the protein levels in malignant melanoma tumor samples, focused on in‐depth histopathology coupled proteome characterization. and localization were determined for a broad spectrum of diverse, surgically isolated tumors originating from multiple body locations. More than 15,500 proteoforms identified by mass spectrometry, which chromosomal subcellular was annotated within both primary metastatic melanoma. data generated global proteomic...
Using several melanoma proteomics data sets we created a single analysis platform that enables the discovery, knowledge build, and validation of diagnostic, predictive, prognostic biomarkers at protein level. Quantitative mass-spectrometry-based proteomic was obtained from five independent cohorts, including 489 tissue samples 394 patients with accompanying clinical metadata. We established an interactive R-based web comparison levels across diverse supports correlation between proteins...
The utilization of PD1 and CTLA4 inhibitors has revolutionized the treatment malignant melanoma (MM). However, resistance to targeted immune-checkpoint-based therapies still poses a significant problem.
A simple methodology for Suzuki–Miyaura cross-coupling reactions using microencapsulated palladium and (het)aryl MIDA boronates in water–alcohol under phosphine-free conditions was developed.
Abstract Background Although older adults are at a high risk of severe or critical Covid-19, there many cases unvaccinated centenarians who had silent infection recovered from mild moderate Covid-19. We studied three Brazilian supercentenarians, than 110 years, survived Covid-19 in 2020 before being vaccinated. Results Despite their advanced age, humoral immune response analysis showed that these individuals displayed robust levels IgG and neutralizing antibodies (NAbs) against SARS-CoV-2....
The discovery of novel protein biomarkers in melanoma is crucial. Our introduction formalin-fixed paraffin-embedded (FFPE) tumor protocol provides new opportunities to understand the progression and open possibility screen thousands FFPE samples deposited biobanks available at hospital pathology departments. In our retrospective biobank pilot study, 90 from 77 patients were processed. Protein quantitation was performed by high-resolution mass spectrometry validated histopathologic analysis....
Introduction While Immune checkpoint inhibition (ICI) therapy shows significant efficacy in metastatic melanoma, only about 50% respond, lacking reliable predictive methods. We introduce a panel of six proteins aimed at predicting response to ICI therapy. Methods Evaluating previously reported two untreated melanoma cohorts, we used published model (EaSIeR score) identify potential distinguishing responders and non-responders. Results Six initially identified the cohort correlated with...
We report here an improved, highly efficient, and general method for the ligand-free Suzuki cross-coupling reaction to synthesis of biaryls, bipyridyls, thienylpyridine, allylphenols. Microwave irradiation (het)aryl halides (hetaryl, allyl)arylboronic acid N-methyl-iminodiacetic (MIDA) ester, using polyurea microencapsulated palladium catalyst (Pd EnCat 30), gave coupling adducts 1a–x in excellent yields just 10–18 min.
Abstract NSD3s, the proline‐tryptophan‐tryptophan‐proline (PWWP) domain‐containing, short isoform of human oncoprotein NSD3, displays high transforming properties. Overexpression NSD3s or yeast protein Pdp3 in Saccharomyces cerevisiae induces similar metabolic changes, including increased growth rate and sensitivity to oxidative stress, accompanied by decreased oxygen consumption. Here, we set out elucidate biochemical pathways leading observed phenotype analyzing alterations metabolome...
Plasma analysis by mass spectrometry-based proteomics remains a challenge due to its large dynamic range of 10 orders in magnitude. We created methodology for protein identification known as Wise MS Transfer (WiMT). Melanoma plasma samples from biobank archives were directly analyzed using simple sample preparation. WiMT is based on MS1 features between several runs together with custom databases ID generation. This entails multi-level database different immunodepletion strategies applying...
Abstract Background Casbene synthase (CS) is responsible for the first committed step in biosynthesis of phorbol esters (PE) Euphorbiaceae. PE are abundant seeds biofuel crop Jatropha curcas and its toxicity precludes use protein-rich cake obtained after oil extraction as an animal feed fumes derived from burning containing also a matter concern. This major hindrance to exploit potential this source raw material produce biodiesel. For reason, current research on J. mainly focused...
Microangiopathy may worsen the clinical outcome of Chagas disease. Given obstacles to investigating dynamics inflammation and angiogenesis in heart tissues parasitized by Trypanosoma cruzi, here we used intravital microscopy (IVM) investigate microcirculatory alterations hamster cheek pouch (HCP) infected green fluorescent protein-expressing T. cruzi (GFP-T. cruzi). IVM performed 3 days post-infection (3 dpi) consistently showed increased baseline levels plasma extravasation. Illustrating...
Abstract The phorbol esters in the seeds of Jatropha curcas are a major hindrance to full exploitation potential this oil crop as source raw material for production biodiesel. Here, various quantitative proteomic strategies used establish proteomes roots, leaves, and endosperm two genotypes J. with contrasting levels seeds. In total 4532, 1775, 503 proteins identified respectively endosperm, comprising 5068 unique proteins; total, 185 differentially abundant 72 20 endosperm. biosynthetic...
Abstract The utilization of PD1 and CTLA4 inhibitors has revolutionized the treatment malignant melanoma (MM). However, resistance to targeted immune-checkpoint-based therapies still poses a significant problem. Here we mine large scale MM proteogenomic data integrating it with cell line dependency screen, drug sensitivity identify druggable targets forecast efficacy resistance. Leveraging protein profiles from established subtypes molecular structures 82 cancer drugs, identified nine...
Abstract While Immune checkpoint inhibition (ICI) therapy shows significant efficacy in metastatic melanoma, only about 50% respond, lacking reliable predictive methods. We introduce a panel of six proteins aimed at predicting response to ICI therapy. Evaluating previously reported two untreated melanoma cohorts, we used published model (EaSIeR score) identify potential distinguishing responders and non-responders. Six initially identified the cohort correlated with predicted cohort....
Summary Malignant melanoma (MM) develops from the melanocytes and in its advanced stage is most aggressive type of skin cancer. Here we report a comprehensive analysis on prospective cohort study, including non-tumor, primary metastasis tissues (n=77) with corresponding plasma samples (n=56) patients malignant melanoma. The tumors surrounding were characterized combination high-throughput analyses quantitative proteomics, phosphoproteomics, acetylomics, whole exome sequencing (WES) combined...
Abstract The novel method using palladium microencapsulated in a polyurea matrix as the catalyst allows Suzuki—Miyaura coupling of aryl halides with arylboronic acid esters (I) and (IV) aqueous ethanol.
Abstract Various functionalized biaryls, (hetaryl)aryls, (hetaryl)pyridines, and allylphenols are efficiently ecofriendly synthesized.