A5081458492- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Botanical Research and Applications
- Seed Germination and Physiology
- Photosynthetic Processes and Mechanisms
- Agricultural pest management studies
- Plant Stress Responses and Tolerance
- Cassava research and cyanide
- Transgenic Plants and Applications
- Lipid metabolism and biosynthesis
- Toxin Mechanisms and Immunotoxins
- Mercury impact and mitigation studies
- Ion channel regulation and function
- Enzyme Production and Characterization
- Phytase and its Applications
- Plant biochemistry and biosynthesis
- Biotin and Related Studies
- Plant Molecular Biology Research
- Nicotinic Acetylcholine Receptors Study
- Heavy Metal Exposure and Toxicity
- Insect Resistance and Genetics
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Neuroscience and Neuropharmacology Research
- Signaling Pathways in Disease
Universidade Federal do Ceará
2014-2025
Universidad Nacional Autónoma de México
2006-2024
University of Naples Federico II
2020
Universidad Católica San Antonio de Murcia
2020
Hospital Clínic de Barcelona
2018
Instituto de Biología Molecular y Celular de Plantas
1996-2016
Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública
2008-2016
Institut d'Investigacions Biomèdiques de Barcelona
2008-2016
Consejo Superior de Investigaciones Científicas
2008-2016
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2009-2016
Abstract Late-Embryogenesis Abundant (LEA) proteins accumulate to high levels during the last stages of seed development, when desiccation tolerance is acquired, and in vegetative reproductive tissues under water deficit, leading hypothesis that these play a role adaptation plants this stress condition. In work, we obtained accumulation patterns Arabidopsis (Arabidopsis thaliana) group 4 LEA different developmental plant organs response deficit. We demonstrate overexpression representative...
Methylmercury (MeHg) is an environmental neurotoxicant whose molecular mechanisms underlying toxicity remain elusive. Here, we investigated events involved in MeHg-induced neurotoxicity cultured cerebellar granule cells (CGCs) as well potential protective strategies for such toxicity. Glutathione peroxidase, isozyme 1 (GPx-1) activity was significantly (p = 0.0017) decreased at 24 h before neuronal death (day vitro 4). This event related to enhanced susceptibilities hydrogen peroxide or...
Abstract Background To date, oil-rich plants are the main source of biodiesel products. Because concerns have been voiced about impact oil-crop cultivation on price food commodities, interest in oil not used for production and amenable to non-agricultural land has soared. As a non-food, drought-resistant crop, Jatropha curcas L. fulfils many requirements biofuel production. Results We generated 13,249 expressed sequence tags (ESTs) from developing germinating seeds. This strategy allowed us...
Cotton ( Gossypium hirsutum L.), a crucial global fibre and oil seed crop faces diverse biotic abiotic stresses. Among these, temperature stress strongly influences its growth, prompting adaptive physiological, biochemical, molecular changes. In this study, we explored the proteomic changes underscoring heat tolerance in leaves of two locally developed cotton genotypes, i.e., tolerant (GH-Hamaliya H tol ) susceptible (CIM-789 sus ), guided by morpho-physiological biochemical analysis. These...
ABSTRACT Hydrophilins are a wide group of proteins whose defining characteristics high hydrophilicity index (> 1.0) and glycine content 6%). The transcripts most hydrophilins accumulate in response to water deficit organisms such as plants, fungi bacteria. In the known Late Embryogenesis Abundant (LEA) belong this (Garay‐Arroyo et al ., Journal Biological Chemistry 275, 5668–5674, 2000). To gain insight into function hydrophilins, an vitro assay was developed which enzymes malate...
In plants, Late Embryogenesis Abundant (LEA) proteins typically accumulate in response to low water availability conditions imposed during development or by the environment. Analogous other organisms are induced when exposed stress conditions. Most of this diverse set can be grouped according properties such as high hydrophilicity and content glycine small amino acids what we have termed hydrophilins. Previously, showed that hydrophilins protect enzyme activities vitro from effects. Here,...
The complete amino acid sequence of the bifunctional α‐amylase/trypsin inhibitor from ragi seeds was determined by analysis fragments and peptides derived protein cleavage with cyanogen bromide digestion trypsin, chymotrypsin, thermolysin, S. aureus V8 protease a Pro‐specific protease. molecular consists single polypeptide chain 122 acids which exhibits homology trypsin inhibitors barley maize an α‐amylase wheat.
Seed longevity is crucial for agriculture and plant genetic diversity, but it limited by cellular damage during storage. Seeds are protected against aging defenses structures such as the seed coat. We have screened an activation-tagging mutant collection of Arabidopsis (Arabidopsis thaliana) selected four dominant mutants with improved (isl1-1D to isl4-1D) under both natural accelerated conditions. In isl1-1D mutant, characterized in this work, overexpression transcription factor ARABIDOPSIS...
Abstract Late Embryogenesis Abundant (LEA) proteins are a group of intrinsically disordered implicated in plant responses to water deficit. In vitro studies revealed that LEA protect reporter enzymes from inactivation during low availability. Group 4 constitute conserved protein family, displaying protective capabilities. Under deficiency or macromolecular crowding, the N-terminal these adopts an alpha-helix conformation. This region has been identified as responsible for activity. study...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPoor correlation between the levels of proteinase inhibitors found in seeds different cultivars cowpea (Vigna unguiculata) and resistance/susceptibility to predation by Callosobruchus maculatusJ. Xavier-Filho, F. A. P. Campos, Maria B. Ary, C. Peres Silva, Marta M. Carvalho, Ligia R. Macedo, J. Lemos, G. GrantCite this: Agric. Food Chem. 1989, 37, 4, 1139–1143Publication Date (Print):July 1, 1989Publication History Published online1 May...
Plant lipoxygenases (LOX, EC 1.13.11.12) have been involved in processes such as stress responses and development. The levels of these enzymes their corresponding mRNAs are modulated during well by different effectors jasmonic acid (JA), its methyl ester (MeJA) or abscisic (ABA). A new lipoxygenase (LOX) cDNA clone, PvLOX2, was isolated from a Phaseolus vulgaris nodule library used to study the LOX mRNA accumulation pattern some developmental stages plants subjected hormone treatments. In...
Abstract Proteinase and amylase activities in larval midguts of the bruchid beetle Zabrotes subfasciatus (Boh.) (Coleoptera: Bruchidae) reared on cowpea ( Vigna unguiculata (L.) Walp.) seeds were investigated. We could detect isolate a proteolytic activity with pH optimum 5.5 (on azo‐casein as substrate) which was activated by thiol reagents inhibited several compounds reactive against ‐SH groups. None plant protein inhibitors serine proteinases utilized effective this activity. This has...
The complete amino acid sequence of the α‐amylase inhibitor I‐2 from ragi seeds was determined by analysis peptides derived protein digestion with trypsin, chymotrypsin thermolysin and S. aureus V8 protease. consists a single polypeptide chain 95 acids, high content serine alanine, no methionine, phenylalanine, histidine or tryptophan. There is homology bifunctional α‐amylase/trypsin in same any inhibitors other plants. contains two regions weakly repetitive internal homology. predicted...
The complete amino acid sequence of the major a subunit lectin from seeds Dioclea grandiflora was determined. deduced analysis peptides derived native α by digestion with trypsin, chymotrypsin, Staphylococcus aureus V8 protease, and pepsin; larger produced citraconylated protein trypsin. consists single polypeptide chain 237 acids which differs concanavalin in 53 positions. Significant levels heterogeneity were observed five positions sequence.
Mass spectrometry has become indispensable for peptide and protein quantification in proteomics studies. When technologies are applied to understand the biology of plants, two-dimensional gel electrophoresis is still prevalent method fractionation, identification, quantitation. In present work, we have used LC–MS compare an isotopic (ICPL) isobaric (iTRAQ) chemical labeling technique quantify proteins endosperm Ricinus communis seeds at three developmental stages (IV, VI, X). Endosperm each...
Summary Light is a major regulator of plant growth and development by antagonizing gibberellins ( GA ), we provide evidence for role light perception in seed coat formation tolerance to deterioration. We have identified two activation‐tagging mutants Arabidopsis thaliana , cog1‐2D cdf4‐1D with improved deterioration linked increased expression COG 1/ DOF 1.5 CDF 4/ 2.3 respectively. These encode homologous transcription factors, 1 most highly expressed seeds. Improved was reproduced...