A5069343764- Glycosylation and Glycoproteins Research
- Polysaccharides Composition and Applications
- Polysaccharides and Plant Cell Walls
- Transgenic Plants and Applications
- Enzyme Production and Characterization
- Biochemical Analysis and Sensing Techniques
- Toxin Mechanisms and Immunotoxins
- Carbohydrate Chemistry and Synthesis
- Sperm and Testicular Function
- Phytase and its Applications
- Nanocomposite Films for Food Packaging
- Proteins in Food Systems
- Phytochemical compounds biological activities
- Bioactive natural compounds
- Collagen: Extraction and Characterization
- Reproductive Biology and Fertility
- Biological Stains and Phytochemicals
- Botanical Research and Applications
- Phytochemistry and Bioactive Compounds
- Plant tissue culture and regeneration
- Agricultural and Food Sciences
- Microbial Metabolites in Food Biotechnology
- Food composition and properties
- Meat and Animal Product Quality
- Legume Nitrogen Fixing Symbiosis
Universidade Federal do Ceará
2015-2025
Universidade de Fortaleza
2015-2025
California Department of Fish and Wildlife
2024
University of California, Davis
2024
Forschungsverbund Berlin
2024
Leibniz Institute for Zoo and Wildlife Research
2024
University of Illinois Urbana-Champaign
2024
Universidade Federal de Uberlândia
2024
Basque Center for Applied Mathematics
2024
Unidade Hospitalar de Bragança
2023
The present study was conducted to identify the major seminal plasma protein profile of boars and its associations with semen criteria. Semen samples were collected from 12 adult subjected evaluation sperm parameters (motility, morphology, vitality, percent cells intact acrosome). Seminal obtained by centrifugation, analyzed two-dimensional SDS-PAGE, proteins identified mass spectrometry (electrospray ionization quadrupole time-of-flight). We tested regression models using spot intensities...
Moringa oleifera Lam is a multipurpose tree cultivated to use as vegetable, for spice, cooking and cosmetic oil medicinal plant. Owing the of its seeds food clarifying agent turbid water some nutritional antinutritional characteristics were studied. The mature contained 332.5 g crude protein, 412.0 fat, 211.2 carbohydrate 44.3 ash per kg dry matter. essential amino acid profile compared with FAO/WHO/UNU scoring pattern requirements different age groups showed deficiency lysine, threonine...
The objective of the present study was to apply polysaccharides from different nontraditional sources for cheese coatings. Chitosan, galactomannan Gleditsia triacanthos, and agar Glacilaria birdiae were tested, with formulations addition plasticizer corn oil. surface properties wetting capacity coatings on determined. three best solutions each polysaccharide chosen, further films cast, permeability water vapor, oxygen, carbon dioxide determined, along opacity. G. triacanthos (formulation:...
High-yielding cowpea (Vigna unguiculata) cultivars were analysed for major changes in seed protein types, amino acid profiles and antinutritional factors content. As usual, the globulins constitute proteins (493.2–573.3 g kg−1 total protein), followed by albumins (201.0–248.0 kg−1), basic glutelins (119.1–154.3 (82.4–92.3 kg−1) prolamins (13.2–20.2 kg−1). The electrophoretic patterns of seeds fractions all resembled to each other both qualitatively quantitatively. However, they showed slight...
A method is presented for the isolation of a lectin from Brazilian cultivar common bean (Phaseolus vulgaris L.), through extraction in acidic (pH 4.2) medium, fractionation with ammonium sulfate, and chromatography on DEAE-cellulose. The was shown to be homogeneous by gel electrophoresis isoelectric focusing.The molecular weight, determined osmometry, 100,250 daltons; point, focusing, pH 5.1; extinction coefficient at 280 nm 7 E(1%) (1 cm) = 7.85.The glycoprotein 9.11% neutral sugars 1.44%...
Neem fruit (Azadirachta indica A. Juss.) are popularly used to treat infections, diarrhea, fever, bronchitis, skin diseases, infected burns and hypertension. Although the antinociceptive anti-inflammatory potential of has already been investigated in experimental models pain inflammation mice, current research is first report evaluation capacity ethanolic extract (EtFrNeem) acute attenuation using adult zebrafish (Danio rerio) as an alternative model use rodents. EtFrNeem was submitted...
We present a review of series contact maps for the determination native interactions in proteins and nucleic acids based on distance threshold. Such are mostly physical chemical construction, yet they sensitive to some parameters (e.g., distances or atomic radii) can neglect key interactions. Furthermore, we also comment new class that only requires geometric arguments. The map is necessary ingredient build robust Go-Martini model their complexes Martini 3 force field. extension popular...
A lectin was isolated fromCanavalia brasiliensis Mart. seeds by combining solubility fractionation with affinity chromatography on Sephadex G-50. The showed a carbohydrate specificity for D-mannose (D-glucose) binding and requirement Ca2+ Mn2+. All the hemagglutinating activity found in cotyledons presence of followed during first 15 days plant germination, through against rabbit erythrocytes, "lectin peak" G-50 chromatography, bands" SDS-polyacrylamide gel electrophoresis arcs rockets"...
The latex of Calotropis procera is a rich source proteolytic activity. This known to contain two distinct cysteine peptidases: procerain and B. In this study, new peptidases were purified from C. latex. enzymes by sequential ion-exchange chromatography steps (CM-Sepharose plus Resource S(®)) at pH 5.0 6.0. had molecular mass spectra corresponding CpCP-1=26,213, CpCP-2=26,133 CpCP-3=25,086 Da. These exhibited discrete differences in terms enzymatic activity broad range temperature conditions...
Acute lymphoblastic leukemia is the most common malignant cancer in childhood. The signs and symptoms of childhood are difficult to recognize, as it not first diagnosis be considered for nonspecific complaints, leading potential uncertainty diagnosis. aim this study was perform proteomic analysis serum from pediatric patients with B-cell acute (B-ALL) identify candidate biomarker proteins, use early evaluation treatment. Serum samples were obtained ten at time (B-ALL group) after induction...
The present study evaluated the effects of heat stress on ram seminal plasma proteome. Six Morada Nova rams were scrotal insulated for 8 days. Scrotal circumference, sperm parameters, and fluid proteins before (Day 0) twice during insulation (Days 4 8), weekly until semen parameters returned to preinsulation values (normal). Seminal analyzed by two-dimensional SDS-PAGE mass spectrometry. circumference decreased from 30 ± 0.4 cm Day 0 22.6 0.6 36 (P < 0.05) became equivalent 71. Motile nearly...
The objective of the present study was to describe relationship seminal plasma and total sperm cell proteins with semen freezability parameters Guzerat bulls. Thirteen bulls were subjected breeding soundness evaluation. Semen samples collected, cryopreserved, then post-thawing kinetics assessed, where high (n = 7) low 6) groups defined. Seminal from 2 separated by 2-dimensional SDS-PAGE, spots identified mass spectrometry. post-cryopreservation as follows in groups, respectively: mean...
Breadfruit (Artocarpus altilis) is a staple food in tropical regions and has several uses. The aim of this study was to establish the nutraceutical potential an enriched protein fraction breadfruit pulp (PFBp). PFBp characterized by Fourier transform infrared spectroscopy, electrophoresis, zymography, thin-layer chromatography, mass spectrometry. toxicity assessed vitro vivo. Its pharmacological effects were determined using zebrafish anxiety models, open field test, light/dark test. rich...
Selectins are essential for leukocyte recruitment in inflammation. Because of a lectin domain present the selectin structure, we investigated anti-inflammtory activity six mannose-glucose binding lectins from brazilian beans: Dioclea guianensis-DguiL; D. grandiflora-DgL; Cratylia floribunda-CfL; violacea-D.vL; virgata-DvirL and Canavalia brasiliensis-ConBr. The were injected intravenously (i.v.) into rats (0.1 1.0 mg/kg; 30 min before irritants) its activities compared to E. coli endotoxin...