A5032424456- Oral microbiology and periodontitis research
- Salivary Gland Disorders and Functions
- Antimicrobial Peptides and Activities
- Dental Health and Care Utilization
- Bone and Dental Protein Studies
- Dental Research and COVID-19
- Dental Erosion and Treatment
- Dental materials and restorations
- Advanced Proteomics Techniques and Applications
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Antifungal resistance and susceptibility
- SARS-CoV-2 detection and testing
- Protein Hydrolysis and Bioactive Peptides
- Biochemical and Structural Characterization
- Oral and gingival health research
- Peptidase Inhibition and Analysis
- Metabolomics and Mass Spectrometry Studies
- Periodontal Regeneration and Treatments
- COVID-19 diagnosis using AI
- Cerebral Palsy and Movement Disorders
- Endodontics and Root Canal Treatments
- Spectroscopy Techniques in Biomedical and Chemical Research
- Asthma and respiratory diseases
- Mosquito-borne diseases and control
University of Saskatchewan
2019-2025
Western University
2012-2024
American Dental Education Association
2021-2023
John Wiley & Sons (United States)
2021-2023
General Services Administration
2023
Universidade Federal de Pelotas
2022
Boston Medical Center
2010
Boston University
2006-2009
Tufts University
2009
Universidade de São Paulo
2002-2007
While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about saliva and its relationship systemic levels. Here, we profiled by enzyme-linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor-binding domain (RBD) serum of acute convalescent patients with laboratory-diagnosed COVID-19 ranging from 3-115 days post-symptom onset (PSO), compared negative controls. Anti-SARS-CoV-2 were readily...
Although SARS-CoV-2 infects the upper respiratory tract, we know little about amount, type, and kinetics of antibodies (Ab) generated in oral cavity response to COVID-19 vaccination. We collected serum saliva samples from participants receiving two doses mRNA vaccines measured level anti-SARS-CoV-2 Ab. detected anti-Spike anti-Receptor Binding Domain (RBD) IgG IgA, as well anti-Spike/RBD associated secretory component most after dose 1. Administration a second boosted but not IgA response,...
The acquired enamel pellicle is a thin protein film forming upon exposure of tooth surfaces to saliva. structural analysis this integument relies on efficient harvesting and identification procedures. Material from three individual subjects two pooled samples yielded the by LC−ESI−MS/MS 130 proteins which 89 were found in or more experiments. A high intersubject consistency composition was observed. Keywords: • proteomics oral mass spectrometry linear ion trap saliva
There is growing interest in the use of human whole saliva for diagnostics and disease monitoring as an alternative to blood samples. In contrast blood, a non-sterile body fluid. Proper hand-ling storage are required preserve integrity potential biomarkers. We investigated salivary autoproteolytic degradation using variety approaches. determined inhibition protease activities by endogenous proteome. addition, stability highly protease-susceptible proteins-histatin 5, statherin, PRP1-was...
Acquired enamel pellicle (AEP) is a protein film that forms on the surface of teeth by selective adsorption proteins and peptides present in mouth. This interface between damage oral biofilm, which modulates attachment bacteria found biofilm. The overall goal this study was to gain insight into biological formation human vivo AEP. hypothesized AEP created successive layers, consist initial binding subsequent protein-protein interactions. hypothesis examined observing quantitative qualitative...
Monitoring of blood glucose is an invasive, painful and costly practice in diabetes. Consequently, the search for a more cost-effective (reagent-free), non-invasive specific diabetes monitoring method great interest. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy has been used diagnosis several diseases, however, applications diabetic treatment are just beginning to emerge. Here, we ATR-FTIR evaluate saliva non-diabetic (ND), (D) insulin-treated (D+I) rats...
AimTo quantify the proteome composition of GCF in periodontal health (HH) and sites with different clinical conditions chronic periodontitis (CP) subjects. Material Methods5 subjects HH 5 CP were submitted to full-mouth examination, sampling. Sites group classified sampled as (P, probing depth, PD>4 mm), gingivitis (G, PD≤3mm bleeding on probing, BOP), healthy (H, without BOP). proteins subjected liquid chromatography electrospray ionization mass spectrometry for identification,...
Recent research efforts in oral biology have resulted elucidation of the proteomes major human salivary secretions and whole saliva. One might hypothesize that proteome minor gland may show significantly different characteristics when compared with parotid or submandibular/sublingual secretions. To test this hypothesis, we conducted first exploration into secretion. Minor secretion was obtained from healthy volunteers, its components were subjected to...
Understanding the composition and structure of acquired enamel pellicle (AEP) has been a major goal in oral biology. Our lab conducted studies on AEP formed permanent enamel. The exhaustive exploration provided comprehensive identification more than 100 proteins from deciduous not subjected to same biochemical characterization scrutiny as that enamel, despite fact is structurally different We hypothesized proteome peptidome may also be composed unique proteins, some which common with...
Although the mature dental biofilm composition is well studied, there very little information on earliest phase of in vivo tooth colonization. Progress collection methodologies and techniques large-scale microbial identification have made new studies this field oral biology feasible. The aim study was to characterize temporal changes diversity cultivable noncultivable microbes early biofilm. Samples were collected from 11 healthy subjects at 0, 2, 4, 6 h after removal plaque pellicle...
Abstract While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about mucosal immune and its relationship systemic levels. Since initially replicates upper airway, oral cavity likely an important parameter that influences course of infection, but how it correlates serum not known. Here, we profile by enzyme linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor binding domain (RBD) (n=496)...
Antibody microarrays have emerged as useful tools for high-throughput protein analysis and candidate biomarker screening. We describe here the development of a multiplexed microsphere-based antibody array capable simultaneously measuring 10 inflammatory mediators. Cytokine-capture microspheres were fabricated by covalently coupling monoclonal antibodies specific cytokines interest to fluorescently encoded 3.1 μm polymer microspheres. An optical fiber bundle containing ∼50 000 individual...