A5028576218- Oral microbiology and periodontitis research
- Dental Health and Care Utilization
- Salivary Gland Disorders and Functions
- Dental Research and COVID-19
- Dental Erosion and Treatment
- Antimicrobial Peptides and Activities
- Fluoride Effects and Removal
- SARS-CoV-2 detection and testing
- Health and Medical Education
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- COVID-19 diagnosis using AI
- Stress and Burnout Research
- Laser Applications in Dentistry and Medicine
- Biochemical and Structural Characterization
- Antifungal resistance and susceptibility
- Educational Outcomes and Influences
- Bone and Dental Protein Studies
- Public Health and Environmental Issues
- Infection Control and Ventilation
- Aging, Health, and Disability
- Periodontal Regeneration and Treatments
- Dental materials and restorations
- Phytochemistry Medicinal Plant Applications
- Respiratory viral infections research
University of Saskatchewan
2020-2025
Universidad El Bosque
2013-2021
Universidade Estadual de Campinas (UNICAMP)
2015-2019
Universidad Nacional de Colombia
2012-2017
Institución Universitaria Colombo Americana
2013-2015
Universidad de Antioquia
2007-2009
While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about saliva and its relationship systemic levels. Here, we profiled by enzyme-linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor-binding domain (RBD) serum of acute convalescent patients with laboratory-diagnosed COVID-19 ranging from 3-115 days post-symptom onset (PSO), compared negative controls. Anti-SARS-CoV-2 were readily...
Abstract While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about mucosal immune and its relationship systemic levels. Since initially replicates upper airway, oral cavity likely an important parameter that influences course of infection, but how it correlates serum not known. Here, we profile by enzyme linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor binding domain (RBD) (n=496)...
Salivary proteins such as histatins (HTNs) have demonstrated critical biological functions directly related to tooth homeostasis and prevention of dental caries. However, HTNs are susceptible the high proteolytic activities in oral environment. Therefore, pH-sensitive chitosan nanoparticles (CNs) been proposed potential carriers protect from enzymatic degradation at physiological salivary pH. Four different types polymers were investigated optimal formulation had good batch reproducibility,...
This study sought to provide an overview of current cariology education in Spanish-speaking Latin American dental schools. Data collection was via eighteen-item survey with questions about curriculum, methods diagnosis and treatment, instructors' perceptions teaching. The response rate 62.1 percent (n=54), distribution participating schools by country as follows: Bolivia (four), Chile Colombia (twenty-four), Costa Rica (one), Cuba Dominican Republic (two), El Salvador Mexico (six), Panama...
To achieve a consensus for an undergraduate cariology teaching curriculum between Colombian dental schools in line with the 2015 Global Alliance Cavity-Free Future goal: '90% of adopting current caries paradigm'.First phase: Four-regional 2-day workshops were conducted 4 representative teachers (cariology, clinics, basic-science, public health and specialisations) from each 24-ACFO (Colombian Dental Schools Association) as follows: Presentations: -Main barriers, -Caries paradigms; -Schools'...
Oral health care settings carry a potentially high risk of causing cross-infection between dentists and patients among dental staff members due to close contact use aerosol-generating procedures. The authors aimed estimate COVID-19 incidence rates Canadian over 6-month period.The conducted prospective cohort study 644 licensed across Canada from July 29, 2020, through February 12, 2021. An online questionnaire, adapted the World Health Organization's Unity Studies protocols for assessment...
Since the modification of proteinaceous components Acquired Enamel Pellicle (AEP) could influence adhesion Streptococcus mutans, most cariogenic bacteria, to dental surfaces, we assessed if engineered salivary peptides would affect adherence and modulate bacterial proteome upon adherence. Single-component AEPs were formed onto hydroxyapatite (HAp) discs by incubating them with statherin, histatin-3, DR9, DR9-DR9, DR9-RR14, RR14, parotid saliva. Then, inoculated S. mutans UA159 bacteria...
Proteomics of human saliva samples was achieved for the first time via biocompatible solid-phase microextraction (bio-SPME) devices. Upon introduction a porogen to conventional C18 coating, porous C18/polyacrylonitrile (PAN) SPME blades were able extract peptides up 3.0 kDa and more than commercial blades. Following Trypsin digestion, salivary proteomic analysis SPME-LC-MS/MS. Seven endogenous proteins consistently identified in all bio-SPME. Taking advantage this strategy, untargeted...
Histatin 5 (Hst5) is an antifungal peptide (AFP) naturally produced by parotid glands with strong activity against Candida albicans. One of its mechanisms action the generation reactive oxygen species (ROS) inside C. albicans cells. Despite being important for human innate immune response, reduced or inactivated proteolytic degradation caused salivary enzymes. To overcome this barrier, we used solid phase synthesis (SPPS) to modify Hst5 amino acid sequence improving and minimizing...
Fluorotic teeth could either be more resistant or susceptible to the caries process than sound ones due their higher enamel fluoride concentration and porosity (subsurface hypomineralization), respectively; however, there is no consensus on this subject. In study, a total of 49 human unerupted third molars presenting Thylstrup Fejerskov (TF) fluorosis scores 0-4 were used. Two slabs obtained from each tooth. The rest tooth crown was powdered, separated dentine. purified powdered enamel,...
The aim of the study was to investigate effect antimicrobial peptides (AMPs) Hylin−a1, KR−12-a5, and Temporin-SHa in Candida albicans as well biocompatibility keratinocytes spontaneously immortalized (NOK-si) human gingival fibroblasts (FGH) cells. Initially, susceptible (CaS—ATCC 90028) fluconazole-resistant (CaR—ATCC 96901) C. strains were grown evaluate each AMP planktonic culture, biofilm, on oral Among AMPs evaluated, temporin−SHa showed most promising results. After 24 h exposure,...
Engineering of the acquired enamel pellicle using salivary peptides has been shown to be a promising anticaries strategy. However, mechanisms by which these protect teeth against tooth decay are not fully understood. In this study, we evaluated effect engineered DR9-DR9 and DR9-RR14 on demineralization in two experimental conditions: (1) adsorbed onto surface forming AEP, (2) AEP combined with their use treat biofilms 2×/day, validated cariogenic Streptococcus mutans vitro biofilm model....
Abstract The detection of SARS-CoV-2 biomarkers by real time PCR (rRT-PCR) has shown that the sensitivity test is negatively affected low viral loads and severity disease. This limitation can be overcome use more sensitive approaches such as mass spectrometry (MS), which not been explored for proteins in saliva. Thus, this study aimed at assessing translational applicability spectrometry-based proteomics to identify saliva from people diagnosed with COVID-19 within fourteen days after...
Introduction: Streptococcus mutans adherence to the tooth surface and subsequent biofilm development is modulated by carbohydrate source, but corresponding effect on bacterial proteome has not been previously studied. This study aimed assess of different carbohydrates S. viability at 2 time points, early attachment (8 h) maturation (24 h). Methods: Hydroxyapatite (HAp) discs coated with parotid saliva proteins were inoculated UA159 in tryptone soy broth without dextrose supplemented one...
We evaluated if the low resistance of fluorotic enamel to demineralization could be overcome by fluoride dentifrice (FD) treatment. Paired slabs sound and (<i>n</i> = 20/group) from human teeth presenting Thylstrup Fejerskov index (TF) scores 0 4 were obtained. Half anatomic surface was isolated used as a control (baseline) regarding mineralization concentration. The submitted pH-cycling model simulating high cariogenic challenge, 2×/day they treated with placebo (PD) or FD...
The dental profession has endured unprecedented disruption amid COVID-19. Novel stressors have included a high risk of occupational exposure to COVID-19, financial losses, and stricter infection prevention control requirements. present study investigated the longitudinal impact COVID-19 on stress anxiety levels cohort Canadian dentists (N = 222) between September 2020 October 2021. Salivary cortisol was selected as biomarker mental stress, 10 sets monthly saliva samples (2,131 in total) were...
The aim of this study was to investigate the atomic composition and proteome salivary proteins adsorbed on surface orthodontic metallic bracket. For this, brackets analyzed with X-ray Photoelectron Spectroscopy (XPS). acquired bracket pellicle characterized after were immersed in human whole saliva supernatant for 2 hours at 37°C. Hydroxyapatite (HA) discs used as a control. Acquired harvested from HA (n = 12) 12). Proteomics based mass spectrometry technology protein identification...
This proof-of-concept study aimed at evaluating the proteolytic profile of histatin 1 and 5 in saliva adolescents with spastic cerebral palsy (CP) gingivitis.This cross-sectional included 24 individuals allocated into three groups: G1 (CP gingivitis; n = 8), G2 (without CP without G3 8). The gingival index (GI) simplified oral hygiene (OHI-S) were evaluated. Whole was collected used to assess rate mode different times. degradation products visualized after cationic PAGE protein band...