A5061421413- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Chemical Synthesis and Analysis
- Lipid Membrane Structure and Behavior
- Protein Hydrolysis and Bioactive Peptides
- Advanced biosensing and bioanalysis techniques
- Marine Invertebrate Physiology and Ecology
- Molecular Junctions and Nanostructures
- Mosquito-borne diseases and control
- Venomous Animal Envenomation and Studies
- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
- Toxin Mechanisms and Immunotoxins
- Marine Sponges and Natural Products
- Polydiacetylene-based materials and applications
- Oral microbiology and periodontitis research
- Mass Spectrometry Techniques and Applications
- RNA Interference and Gene Delivery
- Computational Drug Discovery Methods
- SARS-CoV-2 and COVID-19 Research
- Supramolecular Self-Assembly in Materials
- Electron Spin Resonance Studies
- Plant-Derived Bioactive Compounds
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
Universidade Estadual Paulista (Unesp)
2016-2025
Centro Universitário de Araraquara
2008-2024
Universidade Paulista
2024
Universidade de São Paulo
2005-2024
Universidade Estadual de Santa Cruz
2024
Universidade de Brasília
2007
Universidade Federal de São Paulo
1996-2006
Abstract In the past few years, World Health Organization has been warning that post-antibiotic era is an increasingly real threat. The rising and disseminated resistance to antibiotics made mandatory search for new drugs and/or alternative therapies are able eliminate resistant microorganisms impair development of forms resistance. this context, antimicrobial photodynamic therapy (aPDT) helical cationic peptides (AMP) highlighted treatment localized infections. This study aimed combine AMP...
New variants of SARS-CoV-2 Alpha (B.1.1.7); Beta (B.1.351) Gamma (P.1) and Delta (B.1.617.2) quickly spread in the UK, South Africa, Brazil India, respectively. To address whether mutations RBD spike protein could affect virus infectivity, peptides containing amino acids have been constructed interacted with human ACE2 by computational methods. Our results suggest that K417, E484, L452, T478 N501 are expressively increasing affinity this angiotensin-converting enzyme 2 (ACE2), consequently,...
Owing to fast SARS-CoV-2 mutations, biosensors employing antibodies as biorecognition elements have presented problems with sensitivity and accuracy. To face these challenges, can be replaced the human angiotensin converting enzyme 2 (ACE-2), where it has been shown that affinity between ACE-2 receptor binding domain (RBD) increases emergence of new variants. Herein, we report on Ni-doped ZnO nanorod electrochemical an peptide (IEEQAKTFLDKFNHEAEDLFYQS-NH
Abstract Viral membrane fusion is an orchestrated process triggered by membrane-anchored viral glycoproteins. The S2 subunit of the spike glycoprotein from severe acute respiratory syndrome (SARS) coronavirus (CoV) contains internal domains called peptides (FP) that play essential roles in virus entry. Although has been broadly studied, there are still major gaps molecular details lipid rearrangements bilayer during peptide-membrane interactions. Here we employed differential scanning...
Electrochemical immunosensors offer much in the potential translation of a lab based sensing capability to useful "real world" platform. In previous work we have introduced an impedance-derived electrochemical capacitance spectroscopic analysis as supportive reagentless means reporting on analyte target capture at suitably prepared mixed-component redox-active, antibody-modified interfaces. Herein directly integrate receptive aptamers into redox charging peptide support enabling label-free...
Limitations of the recognition elements in terms synthesis, cost, availability, and stability have impaired translation biosensors into practical use. Inspired by nature to mimic molecular anti-SARS-CoV-2 S protein antibody (AbS) binding site, we synthesized peptide sequence Asn-Asn-Ala-Thr-Asn-COOH (abbreviated as PEP2003) create COVID-19 screening label-free (LF) based on a carbon electrode, gold nanoparticles (AuNPs), electrochemical impedance spectroscopy. The PEP2003 is easily obtained...
ABSTRACT It is well known that cationic antimicrobial peptides (cAMPs) are potential microbicidal agents for the increasing problem of resistance. However, physicochemical properties each peptide need to be optimized clinical use. To evaluate effects dimerization on structure and biological activity Ctx-Ha, we have synthesized monomeric three dimeric (Lys-branched) forms Ctx-Ha by solid-phase synthesis using a combination 9-fluorenylmethyloxycarbonyl (Fmoc) t -butoxycarbonyl (Boc) chemical...
Abstract Recently the peptide Hy‐a1 (IFGAILPLALGALKNLIK), with antimicrobial activity, was isolated from skin secretion of frog Hypsiboas albopunctatus. The aim present work to evaluate four analogues introduction acetyl group, Asp or Lys at N‐terminus Hy‐al supply information about relationship structure–biological activity. activities were assayed by measuring growth inhibition species bacteria and fungus. hemolytic activity also tested. containing Trp instead Leu in position 6 (for...
Ethanol production from sugarcane bagasse requires a pretreatment step to disrupt the cellulose-hemicellulose-lignin complex and increase biomass digestibility, thus allowing obtaining of high yields fermentable sugars for subsequent fermentation. Hydrothermal lime pretreatments have emerged as effective methods in preparing lignocellulosic bioconversion. These are advantageous because they can be performed under mild temperature pressure conditions, resulting less sugar degradation compared...
Antimicrobial peptides (AMPs) can be found in various organisms, and could considered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer exporter of frozen concentrated orange juice world. However, citrus industry has been affected by several diseases such as canker huanglongbing (HLB), caused bacteria Xanthomonas citri subsp. (X.citri) Candidatus Liberibacter asiaticus (CaLas), respectively. In order these putative...
Anticaries agents must interfere with the adhesion of Streptococcus mutans and its proliferation in dental biofilm, without causing host toxicity bacterial resistance. Natural substances, including cationic antimicrobial peptides (CAMPs) their fragments, such as β-defensin-3 peptide fragment (D1-23), have been widely studied. However, chemical physical stability CAMPs may be compromised by external factors, temperature pH, reducing period activity.To overcome aforementioned disadvantage,...
The structural complexity of the nitrogen source strongly affects both biomass and ethanol production by industrial strains Saccharomyces cerevisiae, during fermentation in media containing glucose or maltose, supplemented with a varying from single ammonium salt (ammonium sulfate) to free amino acids (casamino acids) peptides (peptone). Diauxie was observed at low maltose concentrations independent supplementation. At high sugar diauxie not easily observed, growth depended on nature source....
Two new bracelet cyclotides from roots of Pombalia calceolaria with potential anticancer activity have been characterized in this work. The Poca A and B (1 2) the previously known CyO4 (3) were de novo sequenced by MALDI-TOF/TOF mass spectrometry (MS). MS2 spectra examined amino acid sequences determined. purified peptides tested for their cytotoxicity effects on cell migration MDA-MB-231, a triple-negative breast cancer line. isolated reduced number cells more than 80% at 20 μM,...
A new orbitide named ribifolin was isolated and characterized from Jatropha ribifolia using mass spectrometry, NMR spectroscopy, quantitative amino acid analysis, molecular dynamics/simulated annealing, Raman optical activity measurements calculations. Ribifolin (1) its linear form (1a) were synthesized by solid-phase peptide synthesis, followed evaluation of antiplasmodial cytotoxicity activities. Compound 1 moderately effective (IC50 = 42 μM) against the Plasmodium falciparum strain 3D7.
Antimicrobial peptides can be used systemically, however, their susceptibility to proteases is a major obstacle in peptide-based therapeutic development. In the present study, serum stability of p-BthTX-I (KKYRYHLKPFCKK) and (p-BthTX-I)₂, disulfide-linked dimer, were analyzed by mass spectrometry analytical high-performance liquid chromatography (HPLC). activities assessed determining minimum inhibitory concentrations (MIC) using cation-adjusted Mueller-Hinton broth. Furthermore, biofilm...
Infectious diseases are among the leading global causes of death, increasing search for novel antibacterial agents. Among these, biologically active peptides an excellent research tool. Using solid-phase peptide synthesis (SPPS), this work aimed to synthesize derived from C-terminal region Bothropstoxin-I (BthTX-I) (p-BthTX-I, sequence: KKYRYHLKPFCKK), and its disulfide-linked dimeric form, obtained via air oxidation (p-BthTX-I)2. Two other were synthesized evaluate dimerization effect on...
Decapeptide KSL-W shows antibacterial activities and can be used in the oral cavity, however, it is easily degraded aqueous solution eliminated. Therefore, we aimed to develop liquid crystalline systems (F1 F2) for buccal administration treat multispecies biofilms. The were prepared with oleic acid, polyoxypropylene (5) polyoxyethylene (20) cetyl alcohol (PPG-5-CETETH-20), a 1% poloxamer 407 dispersion as oil phase (OP), surfactant (S), (AP), respectively. We characterized them using...