Anticaries agents must interfere with the adhesion of Streptococcus mutans and its proliferation in dental biofilm, without causing host toxicity bacterial resistance. Natural substances, including cationic antimicrobial peptides (CAMPs) their fragments, such as β-defensin-3 peptide fragment (D1-23), have been widely studied. However, chemical physical stability CAMPs may be compromised by external factors, temperature pH, reducing period activity.To overcome aforementioned disadvantage,...

Early childhood caries (ECC) is an aggressive condition that can affect teeth of young children. This study aimed to evaluate genotypic diversity and phenotypic traits S. mutans isolated from dental biofilms children with different status in comparison free (CF) children.Streptococcus strains were supragingival biofilm samples CF, ECC severe-ECC (S-ECC) genotyped by arbitrary-primer polymerase chain reaction - AP-PCR. genotypes tested for their ability reduce the suspension pH through...

This study evaluated the cytotoxicity and effect of fragments derived from three oral cationic peptides (CP): LL-37, D6-17 D1-23 against cariogenic bacteria under planktonic biofilm conditions. For analysis, two epithelial cell lines were used. The minimum inhibitory concentration minimal bactericidal determined for CP control (chlorhexidine-CHX) bacteria. fractional was obtained combinations on Streptococcus mutans. Biofilm assays conducted with best antimicrobial fragment S. results...

Objectives: Addition of chlorhexidine has enhanced the antimicrobial effect glass ionomer cement (GIC) indicated to Atraumatic Restorative Treatment (ART); however, impact this mixture on properties these materials and longevity restorations must be investigated. The aim study was evaluate effects incorporating (CHX) in vitro biological chemical-mechanical GIC vivo clinical/ microbiological follow-up ART with containing or not CHX. Material Methods: For studies, groups were divided into GIC,...

Dental infections can disrupt root development in immature permanent teeth, making traditional endodontic treatment challenging. Apexogenesis, a regenerative approach that promotes natural development, offers potential solution. However, issues related to disinfection and material biocompatibility still remain. The objective of this study was evaluate the synergistic antimicrobial antibiofilm properties double triple antibiotic combinations against common oral pathogens, incorporate most...

This study evaluated the cytotoxicity and antimicrobial activity of analogs cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 hBD-3–1CV chlorhexidine (CHX, control), cell metabolism was MTT. The minimal inhibitory concentration (MIC) bactericidal/fungicidal (MBC/MFC) CHX determined oral pathogens Enterococcus faecalis Streptococcus mutans biofilms cultivated in bovine dentin blocks, different concentrations most...

This study aimed to evaluate the cytotoxicity and synergistic effect of epigallocatechin gallate (EGCG) fosfomycin (FOSFO) on biofilms oral bacteria associated with endodontic infections.This determined minimum inhibitory bactericidal concentration (MIC/MBC) fractionated (FIC) EGCG FOSFO against Enterococcus faecalis, Actinomyces israelii, Streptococcus mutans, Fusobacterium nucleatum. Monospecies multispecies those formed in polystyrene microplates radicular dentin blocks bovine teeth were...

KSL-W peptide has demonstrated antibacterial and antifungal activity inhibitory effects against oral biofilm. This study aimed to check out the effect of chlorhexidine (CLX) or peptide-loaded poloxamer 407-based microemulsions for buccal delivery on Fusobacterium nucleatum (F. nucleatum) The formulation (F) containing 10% copolymer 407 dispersion (1%), 40% oleic acid 50% PPG-5-CETETH-20 was characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), rheology,...

The aim of this research was to evaluate the efficacy a commercial sealing agent at abutment/implant interface against microleakage single and dual-species biofilms Candida albicans Enterococcus faecalis into external hexagon (EH) Morse taper (MT) prosthetic connections. A total 216 samples implants their abutments were tested. Six groups (n = 36) evaluated based on biofilm period incubation (7 14 days). implant connections EH MT 18) divided according use material 9) (EH-T MT-T: with agent;...

The present study evaluated the ability of Bifidobacterium and Lactobacillus species associated with streptococci to increase insoluble extracellular polysaccharide (EPS) production initial caries lesion progression. Bovine enamel blocks (n = 190; 4 mm x mm) were prepared, selected according surface hardness (SH), divided into two groups: a) double combinations: S. mutans or Lactobacillus, b) triple sobrinus species. exposed bacterial associations for 7 days. Subsequently, quantity EPS from...

To evaluate the effect of analogues cationic peptides on viability and expression phenotypic genotypic markers dentin mineralization in MDPC-23 odontoblast-like cells. Cells were exposed to serial dilutions hBD-3-1CV KR-12-a5 compared peptide LL-37 their was assessed by methyltetrazolium assay. Next, (0.78–62.5 μg/mL) applied cells for evaluating total protein (TP) production, alkaline phosphatase (ALP) activity mineralized nodule deposition. Gene (DSPP DMP-1) also determined quantitative...

The objective of the study was to evaluate cytotoxicity and effect curcumin-cinnamaldehyde hybrids (CCHs) on biofilm oral pathogens. Of 18 tested, nine had an inhibitory at least one bacterial species with minimal bactericidal concentrations ranging from 9 625 μg ml−1. CCH 7 promoted a potent against all tested better compatibility than chlorhexidine (CHX). also presented similar or improved over that CHX, causing reduction in metabolism viability single dual-species biofilms. reduced by 86%...

This study aimed to evaluate the effects of flavonoids incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogel on cell viability and mineralization markers odontoblast-like cells. MDPC-23 cells were exposed ampelopsin (AMP), isoquercitrin (ISO), rutin (RUT) control calcium hydroxide (CH) for evaluation viability, total protein (TP) production, alkaline phosphatase (ALP) activity mineralized nodule deposition by colorimetric assays. Based an initial screening, AMP CH loaded PNVCL...

Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in pathogenesis periodontitis. P. has an arsenal specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role initial attachment and organization biofilms. Different genotypes fimA have been related length pathogenicity bacterium. Objectives The aim this study was identify 5 types genotype strains smokers nonsmokers with periodontitis, before after...

This study aimed to evaluate the cytotoxicity and microbiological properties of poly (N-vinylcaprolactam)—PNVCL hydrogels containing flavonoids as intracanal medication for endodontic therapy. Antimicrobial activity ampelopsin (AMP), isoquercitrin rutin was determined against Enterococcus faecalis, Actinomyces israelii, Lactobacillus casei, Streptococcus mutans, Fusobacterium nucleatum by microdilution method. After synthesis characterization rheology, PNVCL were loaded with AMP controls...