A5076335321- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- COVID-19 Impact on Reproduction
- CRISPR and Genetic Engineering
- Infection Control and Ventilation
- Infant Nutrition and Health
- Platelet Disorders and Treatments
- Hemophilia Treatment and Research
- COVID-19 and healthcare impacts
- Influenza Virus Research Studies
- Computational Drug Discovery Methods
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Dermatological and COVID-19 studies
- Virus-based gene therapy research
- Energy Harvesting in Wireless Networks
- Respiratory viral infections research
- Inhalation and Respiratory Drug Delivery
- Vaccine Coverage and Hesitancy
- Biosensors and Analytical Detection
- Breastfeeding Practices and Influences
University of Toronto
2020-2025
Canada Research Chairs
2023
Sunnybrook Research Institute
2020
Institute of Medical Sciences
2020
Rogers (United States)
2020
Sinai Health System
2020
Sunnybrook Health Science Centre
2020
While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about saliva and its relationship systemic levels. Here, we profiled by enzyme-linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor-binding domain (RBD) serum of acute convalescent patients with laboratory-diagnosed COVID-19 ranging from 3-115 days post-symptom onset (PSO), compared negative controls. Anti-SARS-CoV-2 were readily...
Abstract SARS-CoV-2, the virus responsible for COVID-19, has caused a global pandemic. Antibodies can be powerful biotherapeutics to fight viral infections. Here, we use human apoferritin protomer as modular subunit drive oligomerization of antibody fragments and transform antibodies targeting SARS-CoV-2 into exceptionally potent neutralizers. Using this platform, half-maximal inhibitory concentration (IC 50 ) values low 9 × 10 − 14 M are achieved result up 10,000-fold potency enhancements...
<h3>BACKGROUND:</h3> Provision of pasteurized donor human milk, as a bridge to mother's own is the standard care for very low-birth-weight infants in hospital. The aim this research was confirm that Holder pasteurization (62.5°C 30 min) would be sufficient inactivate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) donated milk samples. <h3>METHODS:</h3> We spiked frozen samples from 10 donors Rogers Hixon Ontario Human Milk Bank with SARS-CoV-2 achieve final concentration 1 ×...
Abstract While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about mucosal immune and its relationship systemic levels. Since initially replicates upper airway, oral cavity likely an important parameter that influences course of infection, but how it correlates serum not known. Here, we profile by enzyme linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor binding domain (RBD) (n=496)...
SARS-CoV-2 depends on host cell components for infection and replication. Identification of virus-host dependencies offers an effective way to elucidate mechanisms involved in viral If druggable, factor may present attractive strategy anti-viral therapy. In this study, we performed genome wide CRISPR knockout screens Vero E6 cells four human lines including Calu-3, UM-UC-4, HEK-293 HuH-7 identify genetic regulators infection. Our findings identified only ACE2, the cognate entry receptor, as...
Abstract Since the emergence of SARS-CoV-2 in humans, novel variants have evolved to become dominant circulating lineages. These include D614G (B.1 lineage), Alpha (B.1.1.7), Gamma (P.1), Delta (B.1.617.2), and Omicron BA.1 (B.1.1.529) BA.2 (B.1.1.529.2) viruses. Here, we compared viral replication, pathogenesis, transmissibility these variants. Replication kinetics innate immune response against viruses were tested ex vivo human nasal epithelial cells (HNEC) induced pluripotent stem...
Abstract The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes Coronavirus Disease 2019 (COVID-19), has caused a global pandemic. Antibodies are powerful biotherapeutics to fight viral infections; however, discovery of the most potent and broadly acting clones can be lengthy. Here, we used human apoferritin protomer as modular subunit drive oligomerization antibody fragments transform antibodies targeting SARS-CoV-2 into exceptionally neutralizers. Using this...
Abstract Background The ongoing COVID-19 pandemic has resulted in 185 million recorded cases and over 4 deaths worldwide. Several vaccines have been approved for emergency use humans are being used many countries. However, all the administered by intramuscular injection this may not prevent upper airway infection or viral transmission. Results Here, we describe a novel, intranasally delivered vaccine based on helper-dependent adenoviral (HD-Ad) vector. (HD-Ad_RBD) produces soluble secreted...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of disease 2019 (COVID-19), has been responsible for a global pandemic. Monoclonal antibodies (mAbs) have used as antiviral therapeutics; however, these therapeutics limited in efficacy by viral sequence variability emerging variants concern (VOCs) and deployment need high doses. In this study, we leveraged multi-specific, multi-affinity antibody (Multabody, MB) platform, derived from human apoferritin...
The COVID-19 pandemic has created a global health crisis, with challenges arising from the ongoing evolution of SARS-CoV-2 virus, emergence new strains, and long-term effects COVID-19. Aiming to overcome development viral resistance, our study here focused on developing broad-spectrum pan-coronavirus antiviral therapies by targeting host protein quality control mechanisms essential for replication. Screening an in-house compound library led discovery three candidate compounds cellular...
Abstract The SARS-CoV-2 pandemic is an ongoing threat to global health, and wide-scale vaccination efficient method reduce morbidity mortality. We designed evaluated two DNA plasmid vaccines, based on the pIDV-II system, expressing spike gene, with or without immunogenic peptide, in mice, a Syrian hamster model of infection. Both vaccines demonstrated robust immunogenicity BALB/c C57BL/6 mice. Additionally, shedding infectious virus viral burden lungs was reduced immunized hamsters....
One method that can help slow the spread of coronaviruses is disinfection with UV light. The Delta and Omicron variants COVID-19 virus (SARS-CoV-2) have come to dominate later stages pandemic due their higher rates transmission. In this work, it shown a 17% UV254 dose required for when compared ancestral strain SARS-CoV-2. rate constants SARS-CoV-2 were found be 1.4 ± 0.3, 1.1 0.2, 0.2 cm2/mJ, respectively. statistically different from at 95% confidence level based on least three replicate...
Current vaccine efforts to combat SARS-CoV-2 are focused on the whole spike protein administered as mRNA, viral vector, or subunit. However, receptor-binding domain (RBD) is immunodominant portion of protein, accounting for 90% serum neutralizing activity. In this study, we constructed several versions RBD and together with aluminum hydroxide DDA (dimethyldioctadecylammonium bromide)/TDB (d-(+)-trehalose 6,6'-dibehenate) adjuvant evaluated immunogenicity in mice. We generated human...
Point-of-care tests (POCT) are promising tools to detect SARS-CoV-2 in specific settings. Initial reports suggest the ID NOW™ COVID-19 assay (Abbott Diagnostics Inc, USA) is less sensitive than standard real-time reverse transcription polymerase chain reaction (rRT-PCR) assays. This has raised concern over false negatives POCT. We compared performance of our in-house rRT-PCR assess whether dry swabs used testing could be stored transport media and re-tested by for redundancy provide material...
Le recours aux dons de lait maternel pasteurisé est la norme soins dans les hôpitaux pour nourrissons ayant un très faible poids à naissance, afin faire le pont en attendant que mères puissent allaiter leur enfant. but cette étude était vérifier si pasteurisation l’aide méthode Holder (à 62,5 °C pendant 30 min) serait suffisante inactiver coronavirus du syndrome respiratoire aigu sévère 2 (SRAS-CoV-2) des échantillons provenant donneuses. MÉTHODES: Nous avons inoculé avec SRAS-CoV-2 congelés...
SUMMARY SARS-CoV-2, depends on host cell components for replication, therefore the identification of virus-host dependencies offers an effective way to elucidate mechanisms involved in viral infection. Such factors may be necessary infection and replication SARS-CoV-2 and, if druggable, presents attractive strategy anti-viral therapy. We performed genome wide CRISPR knockout screens Vero E6 cells 4 human lines including Calu-3, Caco-2, Hek293 Huh7 identify genetic regulators Our findings...
Abstract SARS-CoV-2, the causative agent of COVID-19, has been responsible for a global pandemic. Monoclonal antibodies have used as antiviral therapeutics, but limited in efficacy by viral sequence variability emerging variants concern (VOCs), and deployment need high doses. In this study, we leverage MULTI-specific, multi-Affinity antiBODY (Multabody, MB) platform, derived from human apoferritin protomer, to drive multimerization antibody fragments generate exceptionally potent broad...