A5003342338- Advanced Cellulose Research Studies
- Supramolecular Self-Assembly in Materials
- Polysaccharides and Plant Cell Walls
- Bone Tissue Engineering Materials
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Nanocomposite Films for Food Packaging
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Polymer Surface Interaction Studies
- Silk-based biomaterials and applications
- Hydrogels: synthesis, properties, applications
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Chemical Synthesis and Analysis
- Orthopaedic implants and arthroplasty
- Additive Manufacturing and 3D Printing Technologies
- Animal Virus Infections Studies
- Nanoparticles: synthesis and applications
- biodegradable polymer synthesis and properties
- DNA and Nucleic Acid Chemistry
- Supramolecular Chemistry and Complexes
- vaccines and immunoinformatics approaches
National Research Council Canada
2015-2025
National Institute for Nanotechnology
2009-2024
University of Alberta
2009-2024
Alberta Hospital Edmonton
2016
National Academies of Sciences, Engineering, and Medicine
2015
Cellulose nanocrystals (CNCs) continue to gain increasing attention in the materials community as sustainable nanoparticles with unique chemical and mechanical properties. Their nanoscale dimensions, biocompatibility, biodegradability, large surface area, low toxicity make them promising for biomedical applications. Here, we disclose a facile synthesis of poly(2-aminoethylmethacrylate) (poly(AEM)) poly(N-(2-aminoethylmethacrylamide) (poly(AEMA)) CNC brushes via surface-initiated...
The huge worldwide demand for vaccines targeting SARS-CoV-2 has necessitated the continued development of novel improved formulations capable reducing burden COVID-19 pandemic. Herein, we evaluated protein subunit vaccine containing a resistin-trimerized spike antigen, SmT1. When combined with sulfated lactosyl archaeol (SLA) archaeosome adjuvant, induced robust antigen-specific humoral and cellular immune responses in mice. Antibodies had strong neutralizing activity, preventing viral...
In this present work, terminal amino-functionalized nanocrystalline cellulose derivatives were prepared using a simple two-step protecting group-free protocol under aqueous reaction conditions at room temperature. Carboxylate groups first introduced onto the surface of (NCC) via TEMPO-mediated oxidation. Then, surface-carboxylated NCC with bifunctional amines small alkyl chain length by EDC/NHS-mediated coupling furnished desired aminated an amide linkage. Surface covalent functionalization...
Cartilage defects are a persistent issue in orthopedic tissue engineering where acute and chronic damage stemming from osteoarthritis, trauma, sport injuries, present common serious clinical problem. Unlike bone, cartilage repair continues to be largely intractable due the tissue's inherently poor regenerative capacity. Thus, objective of this study is design novel engineered nanostructured scaffold via biologically-inspired self-assembling rosette nanotubes (RNTs) biocompatible non-woven...
Cellulose nanocrystals (CNCs) are renewable nanosized materials with exceptional physicochemical properties that continue to garner a high level of attention in both industry and academia for their potential high-end material applications. These rod-shaped CNCs appealing due non-toxic, carbohydrate-based chemical structure, large surface area, the presence ample hydroxyl groups modifications. CNCs, generally prepared from sulfuric acid-mediated hydrolysis native cellulose, display an anionic...
Using our strongly immunogenic SmT1 SARS-CoV-2 spike antigen platform, we developed antigens based on the Beta & Delta variants of concern (VOC). These elicited higher neutralizing antibody activity to corresponding variant than comparable vaccine formulations original reference strain, while a multivalent generated cross-neutralizing in all three variants. This suggests that current vaccines may be effective at reducing severe disease existing VOC, variant-specific antigens, whether mono-...
The urgency to mitigate global warming and climate change has catalyzed advancements in decarbonization technologies, with membrane separation emerging as a key area of interest. Noted for its compact design,...
Abstract Self‐assembled rosette nanotubes (RNTs), obtained from a twin G∧C base functionalized with lysine‐arginine‐serine‐arginine [KRSR–(G∧C) 2 ], were designed and investigated as bioactive coatings on titanium. These results compared to RNTs derived Lysine–G∧C (K–G∧C), Arg‐Gly‐Asp–G∧C (RGD–G∧C), aminobutane–(G∧C) [AB–(G∧C) ]. The this study revealed that these materials had excellent cytocompatibility properties they enhanced osteoblast (bone forming cell) adhesion when coated In...
Crystalline cellulose nanocrystals (CNCs) have emerged as novel materials for a wide variety of important applications such nanofillers, nanocomposites, surface coatings, regenerative medicine and potential drug delivery. CNCs needle-like structure with sizes in the range 100-200 nm long 5-20 mean aspect ratio 10-100. Despite great applicability CNCs, very little is known about their immunogenicity. Needle-like been to evoke an immune response particular activate (NOD-like receptor, pyrin...
Liposomes composed of sulfated lactosyl archaeol (SLA) have been shown to be a safe and effective vaccine adjuvant with multitude antigens in preclinical studies. In particular, SLA-adjuvanted SARS-CoV-2 subunit vaccines based on trimeric spike protein were immunogenic efficacious mice hamsters. With the continued emergence variants, we sought evaluate next-generation formulations an updated antigenic identity. This was particular interest for widespread Omicron variant, given abundance...
Abstract With the continued transmission of SARS‐CoV‐2 across widely vaccinated populations, it remains important to develop new vaccines and vaccination strategies capable providing protective immunity limiting spread disease. Heterologous prime‐boost based on selection different vaccine formulations administration routes for priming booster doses presents a promising strategy inducing broader immune responses in key systemic respiratory mucosal compartments. Intranasal can induce at site...
Inspired from biological systems, small synthetic organic molecules expressing the hydrogen bonding arrays of DNA bases guanine and cytosine were prepared, their self-assembly into rosette nanotubes (RNTs) was investigated. Due to unique biological, physicochemical, mechanical properties, RNTs could serve as next generation injectable orthopedic materials. In this study, a self-assembling module (termed twin base linkers or TBL) synthesized, corresponding used bioactive components in...
Chiromerism: conformation-dependent supramolecular chirality.
Abstract: Cellulose nanocrystals (CNCs) have emerged as promising candidates for a number of bio-applications. Surface modification CNCs continues to gain significant research interest it imparts new properties the surface design multifunctional CNCs-based materials. A small chemical can potentially lead drastic behavioral changes cell-material interactions thereby affecting intended bio-application. In this work, unmodified were covalently decorated with four different organic moieties such...
Plant genetic engineering offers promising solutions to the increasing demand for efficient, sustainable, and high-yielding crop production as well changing environmental conditions. The main challenge gene delivery in plants is presence of a cell wall that limits transportation genes within cells. Microspores are plant cells are, under right conditions, capable generating embryos, leading formation haploid plants. Here, we designed cationic fluorescent rosette nanotubes (RNTs) penetrate...
Archaeosomes are liposomes formulated using total polar lipids (TPLs) or semi-synthetic glycolipids derived from archaea. Conventional archaeosomes with entrapped antigen exhibit robust adjuvant activity as demonstrated by increased antigen-specific humoral and cell-mediated responses enhanced protective immunity in various murine infection cancer models. However, entrapment efficiency can vary greatly resulting loss during formulation variable antigen:lipid ratios. In order to circumvent...
Vaccines play an important role in maintaining human and animal health worldwide. There is continued demand for effective safe adjuvants capable of enhancing antigen-specific responses to a target pathogen. Rabbit hemorrhagic disease virus (RHDV) highly contagious calicivirus that often induces high mortality rates rabbits. Herein, we evaluated the activity experimental sulfated lactosyl archaeol (SLA) archaeosome adjuvant when incorporated subunit vaccine formulations targeting RHDV. The...
Abstract The next generation skin of wound healing materials should stimulate regeneration by actively promoting appropriate cellular adhesion and proliferation. As with novel self‐assembling solidification properties when transitioning from room to body temperatures, rosette nanotubes (RNTs) may be such a proactive material. RNTs resemble naturally occurring nanostructures in the (such as collagen keratin) assembling noncovalent forces physiological environments. Presenting desirable...