A5074895419- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- CRISPR and Genetic Engineering
- Cell Adhesion Molecules Research
- Hippo pathway signaling and YAP/TAZ
- Chromosomal and Genetic Variations
- Respiratory viral infections research
- Congenital heart defects research
- Genomic variations and chromosomal abnormalities
- Diabetes and associated disorders
- Classical Antiquity Studies
- Archaeology and Historical Studies
- Dermatological and COVID-19 studies
- Protein Degradation and Inhibitors
- DNA Repair Mechanisms
- Extracellular vesicles in disease
- COVID-19 diagnosis using AI
- COVID-19 epidemiological studies
- Angiogenesis and VEGF in Cancer
- Genetics and Neurodevelopmental Disorders
- Protease and Inhibitor Mechanisms
- Power Line Inspection Robots
- Sarcoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
Lunenfeld-Tanenbaum Research Institute
2020-2025
University of Toronto
2020-2024
Mount Sinai Hospital
2020-2024
Sinai Hospital
2024
Sinai Health System
2020-2022
Drexel University
2008
While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about saliva and its relationship systemic levels. Here, we profiled by enzyme-linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor-binding domain (RBD) serum of acute convalescent patients with laboratory-diagnosed COVID-19 ranging from 3-115 days post-symptom onset (PSO), compared negative controls. Anti-SARS-CoV-2 were readily...
Fibrosis is a central pathway that drives progression of multiple chronic diseases, yet few safe and effective clinical antifibrotic therapies exist. In most fibrotic disorders, transforming growth factor–β (TGF-β)–driven scarring an important pathologic feature key contributor to disease progression. Yes-associated protein (YAP) transcriptional coactivator with PDZ-binding motif (TAZ) are two closely related transcription cofactors for coordinating fibrogenesis after organ injury, but how...
Fibrotic diseases account for nearly half of all deaths in the developed world. Despite its importance, pathogenesis fibrosis remains poorly understood. Recently, two mechanosensitive transcription cofactors YAP and TAZ have emerged as important profibrotic regulators multiple murine tissues. this growing recognition, a number questions remain unanswered, including which cell types require YAP/TAZ activation to occur time course activation. Here, we present detailed analysis role that...
Abstract While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about mucosal immune and its relationship systemic levels. Since initially replicates upper airway, oral cavity likely an important parameter that influences course of infection, but how it correlates serum not known. Here, we profile by enzyme linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor binding domain (RBD) (n=496)...
The incidence of infection during SARS-CoV-2 viral waves, the factors associated with infection, and durability antibody responses to among Canadian adults remain undocumented.
Abstract Chromosome instability is highly prevalent in cancer and drives large scale chromosomal imbalances, known as aneuploidies. However, how aneuploidy contributes to tumorigenesis remains difficult study due the vast numbers of genes affected. To address these limitations, we have developed a CRISPR-Knock Out Activation Linked Assay (CRISPR-KOALA), which enables systematic high-throughput bidirectional genetic screens immune-competent mouse models cancer. Using CRISPR-KOALA screened...
Lung morphogenesis requires precise coordination between branching and vascularization to generate distal airways capable of supporting respiration at the cell-cell interface. The specific origins types blood vessels that initially form in lung, however, remain obscure. Herein, we definitively show during early phases lung development [i.e., embryonic day (E) 11.5], functional vessels, replete with flow, are restricted mesenchyme, epithelium. However, by E14.5, response epithelial-derived...
Pancreatic ductal adenocarcinoma (PDAC) is highly resistant to conventional chemotherapy. The presence of both cellular and stromal fibronectin (FN) its interaction with integrins necessary for PDAC progression. We tested the efficacy endothelial monocyte-activating polypeptide II (EMAP II) inhibit progression ability interfere FN-integrin angiogenesis signaling. In heterotopic tumors EMAP caused a significant reduction (>65%) in tumor growth, accompanied by >50% 44% decrease microvessel...
ObjectivesDiabetes is associated with adverse outcomes, including death, after coronavirus disease 19 (COVID-19) infection. Beyond the lungs, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), etiologic agent of COVID-19 pandemic, can infect a range other tissues, kidney, potentially contributing to acute kidney injury in those severe disease. We hypothesized that renal abundance angiotensin-converting enzyme (ACE) 2, cell surface receptor for SARS-CoV-2, may be modulated by...
Background: Canada had substantial mortality from COVID in the first SARS-CoV-2 wave, mostly nursing homes. Efforts to stem pandemic (including vaccination, now being introduced) can benefit direct understanding of relationship age-specific seroprevalence and information on asymptomatic infection.Methods: The Action Beat Coronavirus (Ab-C) study surveyed a reasonably representative sample 19,994 adult Canadians about symptoms analyzed IgG antibodies against self-collected dried blood spots...
Background: The prevalence of infection in Canada's COVID-19 viral waves, the predictors infection, and durability antibody responses to remain undocumented.Methods: We organized serial online surveys a representative group Canadian adults about their COVID experience first (n=19 994; April-July 2020) second wave (n=14 621; October 2020-March 2021). paired these with IgG analysis SARS-CoV-2 seroprevalence self-collected dried blood spots after (n=8967) (n=7102) waves.Findings: cumulative...
Highly mutable pathogens generate viral diversity that impacts virulence, transmissibility, treatment, and thwarts acquired immunity. We previously described C19-SPAR-Seq, a high-throughput, next-generation sequencing platform to detect SARS-CoV-2 we deployed systematically profile variant dynamics of for over 3 years in large, North American urban environment (Toronto, Canada). Sequencing the ACE2 receptor binding motif polybasic furin cleavage site Spike 70,000 patients revealed population...
<title>Abstract</title> Highly mutable pathogens generate viral diversity that impacts virulence, transmissibility, treatment, and thwarts acquired immunity. We previously described C19-SPAR-Seq, a high-throughput, next-generation sequencing platform to detect SARS-CoV-2 we deployed systematically profile variant dynamics of for over 3 years in large, North American urban environment (Toronto, Canada). Sequencing the ACE2 receptor binding motif polybasic furin cleavage site Spike 70,000...
<title>Abstract</title> Chromosome instability is highly prevalent in cancer and drives large scale chromosomal imbalances, known as aneuploidies. However, how aneuploidy contributes to tumorigenesis remains difficult study due the vast numbers of genes affected. Here, we develop a CRISPR-Knock Out Activation Linked Assay (CRISPR-KOALA), enabling high-throughput bidirectional genetic screens immune-competent mouse models cancer. We developed compendium ten most frequently altered human...
Highly mutable pathogens generate viral diversity that impacts virulence, transmissibility, treatment, and thwarts acquired immunity. We previously described C19-SPAR-Seq, a high-throughput, next-generation sequencing platform to detect SARS-CoV-2 we here deployed systematically profile variant dynamics of for over 3 years in large, North American urban environment (Toronto, Canada). Sequencing the ACE2 receptor binding motif polybasic furin cleavage site Spike gene 70,000 patients revealed...