A5101964140- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- Parvovirus B19 Infection Studies
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Hippo pathway signaling and YAP/TAZ
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Transgenic Plants and Applications
- Animal Disease Management and Epidemiology
- Metallurgy and Material Forming
- Viral Infections and Outbreaks Research
- Metal Alloys Wear and Properties
- Biosensors and Analytical Detection
- Vaccine Coverage and Hesitancy
- Advanced biosensing and bioanalysis techniques
- Herpesvirus Infections and Treatments
- Vibrio bacteria research studies
- Cellular transport and secretion
- CRISPR and Genetic Engineering
McGill University
2020-2023
University of Pennsylvania
1966-2020
University of Veterinary Medicine
2019
KIIT University
2016
Centre d’études d’agents Pathogènes et Biotechnologies Pour la Santé
2016
Vaccine design strategies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are focused on the Spike protein or its subunits as main antigen target of neutralizing antibodies. In this work, we propose rapid production methods an extended segment Receptor Binding Domain (RBD) in HEK293SF cells cultured suspension, serum-free media, a major component COVID-19 subunit vaccine under development. The expression RBD, engineered with sortase-recognition motif for protein-based...
Abstract Toxin-antitoxin (TA) modules are two component “addictive” genetic elements found on either plasmid or bacterial chromosome, sometimes both. TA systems perform a wide range of functions like biofilm formation, persistence, programmed cell death, phage abortive infection etc. Salmonella has been reported to contain several such systems. However, the hemolysin expression modulating protein (Hha) and its adjacent uncharacterized hypothetical TomB (previously known as YbaJ), have not...
Abstract Plasmid transfection of mammalian cells is the dominant platform used to produce adeno‐associated virus (AAV) vectors for clinical and research applications. Low yields from this currently make it difficult supply these activities with adequate material. In an effort better understand current limitations transfection‐based manufacturing, study examines what proportion in a model appreciable amounts assembled AAV capsid. Using conformation‐specific antibody staining flow cytometry,...
The influenza A(H1N1)pdm09 virus caused the first pandemic of 21st century. In this study, we wanted to decipher role conserved basic residues viral M1 matrix protein in assembly and release. plays many roles replication cycle. Specifically, it participates particle assembly, can associate with ribonucleoprotein complexes bind cell plasma membrane and/or cytoplasmic tail transmembrane proteins. contains an N-terminal domain 164 amino acids two domains: nuclear localization signal on helix 6...
The ongoing COVID-19 pandemic drew global attention to infectious diseases, attracting numerous resources for development of preparedness plans and vaccine platforms-technologies with robust manufacturing processes that can quickly be pivoted target emerging diseases. Newcastle Disease Virus (NDV) has been studied as a viral vector human veterinary vaccines, but its production relies heavily on embryonated chicken eggs, very few studies producing NDV in cell culture. Here, is produced...
The Ebola virus (EBOV) VP40 matrix protein (eVP40) orchestrates assembly and budding of virions in part by hijacking select WW-domain–bearing host proteins via its PPxY late (L)-domain motif. Angiomotin (Amot) is a multifunctional PPxY-containing adaptor that regulates angiogenesis, actin dynamics, cell migration/motility. Amot also the Hippo signaling pathway interactions with WW-domain–containing effector Yes-associated (YAP). In this report, we demonstrate endogenous crucial for...
New influenza strains are constantly emerging, causing seasonal epidemics and raising concerns to the risk of a new global pandemic. Since vaccination is an effective method prevent spread disease reduce its severity, development robust bioprocesses for producing pandemic vaccines exceptionally important. Herein, membrane chromatography-based downstream processing platform with demonstrated industrial application potential was established. Cell culture-derived virus H1N1/A/PR/8/34 harvested...
Ebola (EBOV) and Marburg (MARV) are members of the Filoviridae family, which continue to emerge cause sporadic outbreaks hemorrhagic fever with high mortality rates. Filoviruses utilize their VP40 matrix protein drive virion assembly budding, in part, by recruitment specific WW-domain-bearing host proteins via its conserved PPxY Late (L) domain motif. Here, we screened an array 115 mammalian, bacterially expressed purified WW-domains using a PPxY-containing peptide from MARV (mVP40) identify...
A challenge of any biosensing technology is the detection very low concentrations analytes. The fluorescence interference contrast (FLIC) technique improves fluorescence-based sensitivity by selectively amplifying, or suppressing, emission a fluorophore-labeled biomolecule immobilized on transparent layer placed top mirror basal surface. standing wave reflected light means that height operates as surface-embedded optical filter for signal. FLIC extreme to wavelength also its main problem:...
Abstract Background Adeno‐associated viruses (AAVs) are gaining interest in the development of cellular immunotherapy. Compared to other viral vectors, AAVs can reduce risk insertional oncogenesis. AAV serotype 6 (AAV6) shows highest efficiency for transducing T cells. Nevertheless, a multiplicity infection (MOI) up one million genomes per cell is required transduce target cells effectively. Cell‐penetrating peptides (CPPs) short, positively charged that easily translocate plasma membranes...
There have been considerable efforts on improving the lentiviral vector (LV) system and their production. However, there remains persisting challenge of producing a sufficient quantity LVs at manufacturing scale to support treatments beyond early clinical trials. Furthermore, innately labile nature poses an equally important obstacle in LV As lose function over time they are sensitive environmental factors each unit operation bioprocess workflow, integrated continuous is attractive strategy...
A total 64 numbers of dogs (36 male and 28 female) were divided age wise into three groups viz. group (1-3 months), B (4-12 months) C (more than 12 with 24, 26 14 animals in the said respectively sex exotic (52 animals) indigenous breeds (12 to study therapeutic alternations sero-biochemical parameters electrolyte concentration Canine Parvo Virus infection after treatment conventional method administration hyperimmune sera.Further, included small (10 animals), medium (6 large sized dogs.It...
Plasmid transfection of mammalian cells is the dominant platform used to produce adeno-associated virus (AAV) vectors for clinical and research applications. Low yields from this currently make it difficult supply these activities with adequate material. In an effort better understand current limitations transfection-based manufacturing, study examines what proportion in a model appreciable amounts assembled AAV capsid. Using conformation-specific antibody staining flow cytometry we report...
The human cell line HEK293 is one of the preferred choices for manufacturing therapeutic proteins and viral vectors applications. Despite its increased use, it still considered in disadvantage production aspects compared to lines such as CHO line. We provide here a simple workflow rapid generation stably transfected cells expressing an engineered variant SARS-CoV-2 Receptor Binding Domain (RBD) carrying coupling domain linkage VLPs through bacterial transpeptidase-sortase (SrtA). To generate...