A5038581405- Salmonella and Campylobacter epidemiology
- Gut microbiota and health
- Bacteriophages and microbial interactions
- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- HIV-related health complications and treatments
- Escherichia coli research studies
- Cancer Research and Treatments
- Clostridium difficile and Clostridium perfringens research
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS Research and Interventions
- Immunotherapy and Immune Responses
- HIV/AIDS oral health manifestations
- RNA Interference and Gene Delivery
- Salivary Gland Disorders and Functions
- Probiotics and Fermented Foods
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiovascular Syncope and Autonomic Disorders
- Bacterial Genetics and Biotechnology
- Aquaculture disease management and microbiota
- Identification and Quantification in Food
- Cancer therapeutics and mechanisms
- HIV/AIDS drug development and treatment
- Trace Elements in Health
Karolinska Institutet
2020-2024
Karolinska University Hospital
2023
Science for Life Laboratory
2023
National Bioinformatics Infrastructure Sweden
2023
Lund University
2023
ICMR-National Institute of Virology
2022-2023
KIIT University
2014-2022
Umeå University
2020-2021
KPC Medical College and Hospital
2005
École Normale Supérieure - PSL
1997
We report here a Nipah virus (NiV) outbreak in Kozhikode district of Kerala state, India, which had caused fatal encephalitis 12-year-old boy and the response, led to successful containment disease related investigations. Quantitative real-time reverse transcription (RT)-PCR, ELISA-based antibody detection, whole genome sequencing (WGS) were performed confirm NiV infection. Contacts index case traced isolated based on risk categorization. Bats from areas near epicenter sampled for throat...
Salmonella enterica serovar Typhimurium (S. Typhimurium) is one of the leading causative agents food-borne bacterial gastroenteritis. Swift invasion through intestinal tract and successful establishment in systemic organs are associated with adaptability S. to different stress environments. Low-pH serves as first lines defense mammalian hosts, which must efficiently overcome establish an infection. Therefore, a better understanding molecular mechanisms underlying acid highly relevant. In...
Millions of individuals currently living with HIV globally are receiving antiretroviral therapy (ART) that suppresses viral replication and improves host immune responses. The involvement gut microbiome during infection has been studied, exposing correlation status inflammation. However, the direct effect ART on commensals HIV-infected mostly overlooked in studies. We used 16S rRNA sequencing (Illumina MiSeq) for determining microbiota composition stool samples from 16 viremic patients...
Gut microbiome plays a significant role in HIV-1 immunopathogenesis and HIV-1-associated complications. Previous studies have mostly been based on 16S rRNA gene sequencing, which is limited taxonomic resolution at the genus level inferred functionality. Herein, we performed deep shotgun metagenomics study with aim to obtain more precise landscape of gut dysbiosis infection. A reduced tendency alpha diversity significantly higher beta were found HIV-1-infected individuals antiretroviral...
Abstract The gut and oral microbiome is altered in people living with HIV (PLWH). While antiretroviral treatment (ART) pivotal restoring immune function PLWH, several studies have identified an association between specific antiretrovirals, particularly integrase inhibitors (INSTI), weight gain. In our study, we explored the differences microbiota of PLWH under different ART regimens, its correlation to Body Mass Index (BMI). Fecal salivary samples were collected from (n = 69) healthy...
Abstract Toxin-antitoxin (TA) modules are two component “addictive” genetic elements found on either plasmid or bacterial chromosome, sometimes both. TA systems perform a wide range of functions like biofilm formation, persistence, programmed cell death, phage abortive infection etc. Salmonella has been reported to contain several such systems. However, the hemolysin expression modulating protein (Hha) and its adjacent uncharacterized hypothetical TomB (previously known as YbaJ), have not...
Abstract Although mRNA SARS-CoV-2 vaccines are generally safe and effective, in certain immunocompromised individuals they can elicit poor immunogenic responses. Among these individuals, people living with HIV (PLWH) have immunogenicity to several oral parenteral vaccines. As the gut microbiome is known affect vaccine immunogenicity, we investigated whether baseline microbiota predicts immune responses BNT162b2 healthy controls PLWH after two doses of BNT162b2. Individuals high spike IgG...
Salmonella enterica subsp. I serovar Enteritidis (S. Enteritidis), one of the causative agents for non-typhoidal gastrointestinal diseases in humans is an intracellular bacterium and mechanism its invasion into host cells critical to cause infection. The virulence pathogen explained by expression genes located on pathogenicity islands, mostly encoded under SPI-1 SPI-2. However, S. Typhimurium SL1344, despite sharing ∼98% genome with P125109, lacks few regions differences (ROD) that are...
HIV-1 elite controllers (EC) are a rare group among HIV-1-infected individuals who can naturally control viral replication for prolonged period. Due to their heterogeneous nature, no universal mechanism could be attributed the EC status; instead, several host and factors have been discussed as playing role. In this study, we investigated fecal metabolome microbiome in Swedish cohort of (n = 14), treatment-naive viremic progressors (VP; n 16), HIV-negative (HC; 12). Fecal untargeted...
Non-typhoidal Salmonella (NTS) infections caused primarily by S. Enteritidis and Typhimurium particularly in immunocompromised hosts have accounted for a large percentage of fatalities developed nations. Antibiotics revolutionized the cure enteric but also led to rapid emergence pathogen resistance. New powerful therapeutics involving metabolic enzymes are expected be potential targets combating microbial ensuring effective health management. Therefore, need new antimicrobials fight such...
Non-typhoidal Salmonella (NTS) infections are emerging as leading problem worldwide and the variations in host immune status append to concern of NTS. enterica serovar Typhimurium is one causative agents NTS has been extensively studied. The inactivation pathogenicity island 2 (SPI2) encoded type-III secretion system (TTSS2) reported rendering strain incapable for systemic dissemination sites also proposed live-attenuated vaccine. However, from TTSS2-deficient have reported. In this study,...
Salmonella enterica serovar Enteritidis, the most common cause of human gastroenteritis, employs several virulence factors including lipopolysaccharide (LPS) for infection and establishment disease inside host. The LPS S. Enteritidis consists lipid A, core oligosaccharide O-antigen (OAg). OAg repeating units containing different sugars. sugars are synthesized assembled by a set enzymes encoded genes organized into clusters. Present study focuses on effect deletion involved in biosynthesis...
Salmonella enterica serovar Enteritidis (S. Enteritidis; wild type (WT)) is a major cause of foodborne illness globally. The ability this pathogen to survive stress inside and outside the host, such as encountering antimicrobial peptides heat stress, determines efficiency enteric infection. These stressors concertedly trigger virulence factors encoded on pathogenicity islands (SPIs). Although RpoS well-known central transcriptional regulator, functional information regarding genes regulon...
Coevolution of microbiome and immunity at mucosal sites is essential for our health. Whether the oral microbiome, second largest community after gut, contributes to immunogenicity COVID-19 vaccines not known. We investigated baseline in individuals COVAXID clinical trial receiving BNT162b2 mRNA vaccine. Participants (n=115) included healthy controls (HC; n=57) people living with HIV (PLHIV; n=58) who met study selection criteria. Vaccine-induced Spike antibodies saliva serum from 0 6 months...
Enhanced levels of a dipeptide, WG-am, have been reported among elite controllers - patients who spontaneously control their HIV-1 infection. This study aimed to evaluate anti-HIV-1 activity and mechanism action WG-am.Drug sensitivity assays in TZM.bl cells, PBMCs ACH-2 cells using WT mutated strainswere performed the antiviral WG-am. Mass spectrometry-based proteomics Real-time PCR analysis reverse transcription steps were unravel second WG-am.The data suggest that WG-am binds CD4 binding...
Enteric microbial pathogenesis, remarkably a complex process, is achieved by virulence factors encoded genes located within regions of the bacterial genome termed pathogenicity islands. Salmonella islands (SPI) encodes proteins, that are essential determinants for pathogen colonization and virulence. In addition to well-characterized SPI-1 SPI-2 which required invasion intracellular replication, respectively, SPI-6 (formerly known as enterica centisome 7 island [SCI]) encoding proteins also...
Summary Thirty‐two faecal samples from various pet chelonians were bacteriologically examined using combinations of selective media for Salmonella detection. Strains identified as clustered by rRNA gene restriction pattern (ribotyping) analysis prior to serological typing. The combination selenite broth and Rambach or Salmonella‐Shigella agars gave the maximum rate recovery. Of 32 examined, 13 (40%), positive Salmonella. Five serovars identified, Muenchen, Rubislaw, Newport, Ferruch a...
Abstract Although mRNA SARS-CoV-2 vaccines are generally safe and effective, in certain immunocompromised individuals they can elicit poor immunogenic responses. Among these individuals, people living with HIV (PLWH) have immunogenicity to several oral parenteral vaccines. As the gut microbiome is known affect vaccine immunogenicity, we investigated whether baseline microbiota predicts immune responses BNT162b2 healthy controls PLWH after two doses of BNT162b2. Individuals high spike IgG...
Abstract Background : The gut and oral microbiome is altered in people living with HIV (PLWH). While antiretroviral treatment (ART) pivotal restoring immune function PLWH, several studies have identified an association between specific antiretrovirals, particularly integrase inhibitors (INSTI), weight gain. In our study, we explored the differences microbiota of PLWH under different ART regimens, its correlation to Body Mass Index (BMI). Methods Fecal salivary samples were collected from...
Salmonella, like other pathogenic bacteria has undergone multiple genomic alterations to adapt itself into specific host environments executing varied degrees of virulence through evolution. Such variations in genome content have been assumed lead the closely related non-typhoidal serovars, S. Enteritidis, and Typhimurium exhibit Type Three Secretion System −2 (T3SS-2) based diverse colonization inflammation kinetics. Mutually exclusive genes present either serovars are recently being...
We report here a study of platelet aggregation in diabetes, induced by epinephrine and its inhibition yohimbine hydrochloride (YH), an alpha(2)-adrenergic receptor-blocking agent. Interestingly, emergence spontaneous macroaggregation (SPMA) was observed six out 75 cases the absence any agonist. The SPMA were strongly associated with insensitivity to YH (in contrast non-SPMA cases) when used as suggest that correlation is result over expression alpha(2)-adrenoceptors such subjects....