A5046765429- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Rabies epidemiology and control
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Bartonella species infections research
- Mycobacterium research and diagnosis
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Infectious Diseases and Mycology
- Bacillus and Francisella bacterial research
- Vector-borne infectious diseases
- Tuberculosis Research and Epidemiology
- Viral Infections and Vectors
- Enterobacteriaceae and Cronobacter Research
- Liver Disease and Transplantation
- Antibiotic Use and Resistance
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Animal Disease Management and Epidemiology
- Microbial Inactivation Methods
- Insect Resistance and Genetics
University Hospital Frankfurt
2020-2025
Goethe University Frankfurt
2020-2025
Landesbetrieb Landwirtschaft Hessen
2025
Hessian Agency for Nature Conservation, Environment and Geology
2024
Supply Chain Competence Center (Germany)
2020-2022
Justus-Liebig-Universität Gießen
2014-2019
German Center for Infection Research
2016-2019
Philipps University of Marburg
2018
Centers for Disease Control and Prevention
2018
BackgroundSince the beginning of war in Ukraine February 2022, Ukrainians have been seeking shelter other European countries.AimWe aimed to investigate prevalence and molecular epidemiology multidrug-resistant Gram-negative (MDRGN) bacteria meticillin-resistant Staphylococcus aureus (MRSA) Ukrainian patients at admittance University Hospital Frankfurt, Germany.MethodsWe performed screening observational analysis all from March until June 2022. Genomes MDRGN isolates were analysed for...
Abstract Nosocomial pathogens can cause life-threatening infections in neonates and immunocompromised patients. E. bugandensis (EB-247) is a recently described species of Enterobacter , associated with neonatal sepsis. Here we demonstrate that the extended spectrum ß-lactam (ESBL) producing isolate EB-247 highly virulent both Galleria mellonella mouse models infection. Infection studies streptomycin-treated model showed as efficient Salmonella Typhimurium inducing systemic infection release...
Objectives Rising prevalence of multidrug-resistant organisms (MDRO) is a major health problem in patients with liver cirrhosis. The impact MDRO colonization transplantation (LT) candidates and recipients on mortality has not been determined detail. Methods Patients consecutively evaluated listed for LT tertiary German transplant center from 2008 to 2018 underwent screening including methicillin-resistant Staphylococcus aureus (MRSA), gram-negative bacteria (MDRGN), vancomycin-resistant...
Salmonella enterica serovar Typhimurium (S. Typhimurium) is one of the leading causative agents food-borne bacterial gastroenteritis. Swift invasion through intestinal tract and successful establishment in systemic organs are associated with adaptability S. to different stress environments. Low-pH serves as first lines defense mammalian hosts, which must efficiently overcome establish an infection. Therefore, a better understanding molecular mechanisms underlying acid highly relevant. In...
Cellular sensing of bacterial RNA is increasingly recognized as a determinant host-pathogen interactions. The intracellular pathogen Listeria monocytogenes induces high levels type I interferons (alpha/beta [IFN-α/β]) to create growth-permissive microenvironment during infection. We previously demonstrated that RNAs secreted by L. (comprising the secRNome) are potent inducers IFN-β. determined composition and diversity members secRNome found they uniquely enriched for noncoding small...
Listeria monocytogenes can cause severe illness in individuals with weakened immune systems. In March 2023, L. was isolated from blood (n = 2) or pleural fluid 1) of three febrile patients receiving synthetic glucocorticoids a tertiary hospital Germany. Food supply records suggested sliced parboiled sausage as the likely source, and four samples sealed packaged sausages ham one manufacturer. The patient food isolates clustered within 0–4 allelic differences. Counts all were < 100...
Abstract Nitroxoline is a bacteriostatic quinoline antibiotic, known to form complexes with metals. Its clinical indications are limited uncomplicated urinary tract infections, susceptibility breakpoint only available for Escherichia coli . Here, we test > 1000 isolates and demonstrate much broader activity spectrum species-specific bactericidal activity, including Gram-negative bacteria which therapeutic options due multidrug resistance. By combining genetic proteomic approaches direct...
Phenotypic (Sensititre Myco, pDST) and genotypic drug susceptibility testing (GenoType NTM DR, gDST) in M. avium complex (MAC) have become available as standardized assays, but comparable data is needed. This study aimed to investigate the phenotypic patterns MAC clinical isolates.Overall, 98 isolates from 85 patients were included. pDST gDST performed on all results compared regarding specificity sensitivity using a reference method. The impact of instability was studied biological assay...
After testing ceftolozane-tazobactam, ceftazidime-avibactam, and cefiderocol against a collection of 233 multidrug-resistant (MDR) Pseudomonas aeruginosa , we showed that is the most active antipseudomonal β-lactam agent (susceptibility rates were 46.6%, 48.4%, 97.4%, respectively). The prevalent one was sequence type 235 (ST235) (24.7%), followed by ST244, ST175, ST233, with all belonging to top 10 P. high-risk clones worldwide distribution.
Mycobacterium abscessus is a highly antibiotic-resistant opportunistic pathogen causing clinically challenging infections in patients with preexisting lung diseases or under immunosuppression. Hence, reliable antibiotic susceptibility data are required for effective treatment. Aims of this study were to investigate (i) the congruence genotypic and phenotypic antimicrobial testing, (ii) relationship between resistance profile clinical course, (iii) phylogenetic relations M. German patient cohort.
Listeria monocytogenes is a bacterial pathogen and causative agent for the foodborne infection listeriosis, which mainly threat pregnant, elderly, or immunocompromised individuals. Due to its ability invade colonize diverse eukaryotic cell types including cells from invertebrates, L. has become well-established model organism intracellular growth. Almost 10 years ago, we others presented first whole-genome microarray-based transcriptome of monocytogenes. With advent newer technologies...
Lassa virus (LASV) is a zoonotic, hemorrhagic fever-causing endemic in West Africa, for which no approved vaccines or specific treatment options exist. Here, we report the genome sequence of LASV isolated from first case acquired fever disease outside Africa.
Abstract Nitroxoline is a bacteriostatic quinoline antibiotic, considered metal chelator inhibiting the activity of RNA-polymerase 1 . Its clinical indications are limited to uncomplicated urinary tract infections (UTIs), with susceptibility breakpoint only available for Escherichia coli 2 By testing > 1,000 isolates, here we demonstrate much broader spectrum and species-specific bactericidal activity, including multidrug-resistant Gram-negative bacteria which therapeutic options due...
Objective Multidrug-resistant organisms (MDRO), especially carbapenem-resistant (CRO), represent a threat for newborns. This study investigates the colonization prevalence of these pathogens in newborn unit at Kenyan tertiary hospital an integrated approach combining routine microbiology, whole genome sequencing (WGS) and surveillance data. Methods The was performed Kenyatta National Hospital (KNH) 2019 over four-month period included 300 mother-baby pairs. A total 1,097 swabs from newborns...
microRNAs (miRNAs) coordinate several physiological and pathological processes by regulating the fate of mRNAs. Studies conducted in vitro indicate a role control host-microbe interactions. However, there is limited understanding miRNA functions vivo models bacterial infections. In this study, we systematically explored changes expression levels Galleria mellonella larvae (greater-wax moth), model system that recapitulates vertebrate innate immunity, following infection with L....
Acinetobacter baumannii is an antibiotic-resistant, Gram-negative pathogen that causes a multitude of nosocomial infections. However, pathogenicity mechanisms and the host cell response during infection remain unclear. In this study, we determined virulence traits A. clinical isolates belonging to most widely disseminated international clonal lineage, cluster 2 (IC2), in vitro vivo. Complexome profiling primary human endothelial cells with revealed mitochondria, particular complexes electron...
Bartonella bacilliformis is the aetiological agent of Carrión's disease, a biphasic and highly lethal illness formerly restricted to South American Andes that now spreading adjacent areas. Reliable serodiagnostic approaches vaccines are urgently needed. In this study, we aimed identify immunodominant proteins B establish novel reliable tools.We used reverse vaccinology approach in combination with an analysis heterologous genomic expression libraries proteins, on basis genome sequences...
Bartonella bacilliformis is the biological agent of Carrion's disease, a vector-borne, life-threatening human bartonellosis restricted to South America. Here, we report complete genome sequence B. KC584 (ATCC 35686). Although it commonly used as reference strain, date, its has not been published.
Background: Carbapenem-resistant Gram-negative bacteria (CRGN) cause life-threatening infections due to limited antimicrobial treatment options. The occurrence of CRGN is often linked hospitalization and but remains incompletely understood. are common in patients with severe illness (e.g., liver transplantation patients). Using whole-genome sequencing (WGS), we aimed elucidate the evolution this vulnerable cohort reconstruct potential transmission routes. Methods: From 351 evaluated for...
We describe a case of endocarditis caused by Streptobacillus moniliformis bacteria, known cause rat-bite fever, in 32-year-old woman with pet rats Germany. The patient had strong serologic response, high IgM and IgG titers. Serologic analysis is promising tool to identify S. bacterial infection.
ABSTRACT We report here a complete genome sequence of Ebola virus Makona from nonfatal patient sample that originated in Sierra Leone during the last outbreak West Africa (species Zaire ebolavirus ) using highly accurate circle sequencing (Cir-seq) method.
Bartonella alsatica causes bacteremia in rabbits and, rarely, human infections. Here, we announce the complete and closed genome of B. IBS 382 (CIP 105477), generated by long-read Pacific Biosciences single-molecule real-time (SMRT) sequencing. The availability this sequence allows future work on understanding zoonotic potential pathogen.