A5090094056- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Analytical Chemistry and Chromatography
- Peptidase Inhibition and Analysis
- Protein purification and stability
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Antimicrobial Peptides and Activities
- Aquaculture disease management and microbiota
- Marine Invertebrate Physiology and Ecology
- RNA and protein synthesis mechanisms
- Acute Myeloid Leukemia Research
- Protein Kinase Regulation and GTPase Signaling
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Microbial infections and disease research
- Toxin Mechanisms and Immunotoxins
- Machine Learning in Bioinformatics
- Bacterial Infections and Vaccines
Centro de Ingeniería Genética y Biotecnología
2016-2025
National Center for Genetic Engineering and Biotechnology
1992-2024
Universidade Federal do Rio de Janeiro
2013
European Bioinformatics Institute
2013
National University of Quilmes
2010
Keyhole limpet haemocyanins (KLH1 and KLH2) from Megathura crenulata , are multi-subunit oxygen-carrying metalloproteins of approximately 3900 amino acids, that widely used as carrier proteins in conjugate vaccines immunotherapy.
Abstract Proteome-wide scale in a dose – and time-depending setting is crucial to fully understand the pharmacological mechanism of anticancer drugs as well identification candidates for drug response biomarkers. Here, we investigated effect CIGB-300 peptide at IC 50 80 doses during 1 4 h treatment on squamous lung cancer cell (NCI-H226) proteome. An overwhelming dose-dependent inhibitory with minor up-regulated proteins was observed by increasing level. Functional enrichment also common or...
SeqMS, a software aid for de novo sequencing by tandem mass spectrometry (MS/MS), which was initially developed the automated interpretation of high-energy collision-induced dissociation (CID) MS/MS spectra peptides, has been applied to low-energy CID and post-source decay (PSD) peptides. Based on peptide backbone fragmented ions their related ions, are dominant observed in latter two techniques, types propensities be have optimized efficient spectra. In typical example, modified SeqMS...
Spectra obtained by low-energy electrospray ionization tandem mass spectrometry (ESI-MS/MS) of 34 peptides containing aspartic acids at position n were studied and unambiguously differentiated. β-Aspartic acid yields an internal rearrangement similar to that the C-terminal rearrangements protonated cationized peptides. As a result this rearrangement, two different ions N- ends original peptide are formed, namely, b − 1 + H2O y″ℓ 46 ions, respectively, where ℓ is number amino residues in...
A combination of antiviral drugs known as antiretroviral therapy (ART) has shown effectiveness against the human immunodeficiency virus (HIV). ART markedly decreased mortality and morbidity among HIV-infected patients, having even reduced HIV transmission. However, an important current disadvantage, resistance development, remains to be solved. Hope is focused on developing cellular targets. This strategy expected prevent emergence viral resistance. In this study, using a comparative...
Maleimide thiol chemistry is widely used for the synthesis of conjugate vaccines and antibody-drug-conjugates. Proteolysis a vaccine mainly generates linear type 2 peptides (cross-linked peptides) with thiosuccinimide linker its stabilized forms (thiazine, and/or hydrolyzed thiosuccinimide). Type contain valuable information on conjugation sites. Cleavable crosslinkers could make more reliable sites identifications, but based different chemistry, they are to study protein-protein...
Internal rearrangement involving the loss of C-terminal amino acid residue upon collision-induced dissociation (CID) or metastable decomposition was studied for protonated peptides. To investigate structural characteristics peptides responsible this rearrangement, a series synthetic were prepared and subjected to B/E-linked scan tandem mass spectrometric analyses using four-sector instrument. The results showed that position basic in peptide sequence its basicity have significant influence...
Nowadays, the studies of pituitary adenylate cyclase-activating polypeptide (PACAP)-related peptide (PRP) and PACAP in non-mammalian vertebrates, especially fish, have paid attention mainly to localization, cloning, structural evolution peptides, but very little is known about its biological functions as growth-promoting factors low vertebrates. In this work, we cloned characterized PRP/PACAP cDNA from commercially important North African catfish Clarias gariepinus. The sequence obtained...
SeqMS, a software program designed for the automated interpretation of high-energy collision-induced dissociation (CID) mass spectra singly protonated peptides ionized by fast atom bombardment, has been developed. The is capable probing sequence an unknown peptide, and even certain modified peptides. program, compiled WINDOWS95 or NT, also permits retrieval raw data reconstruction on user-friendly graphical interface with aid several tools processing spectra, which include setting multiple...
Tamoxifen is the most frequently used drug for hormone therapy of breast cancer patients, even though a high percentage women are (or become) refractory to this treatment. The proteins involved in tamoxifen resistance tumor cells as well mechanisms by which they interact, still unknown. Some years ago, we established xenograft 3366, sensitive and 3366/TAM, resistant tamoxifen, derived after two vivo passages parental 3366 under Here, compare protein expression levels both xenografts. 2-DE...
CIGB-300 is a proapoptotic peptide-based drug that abrogates the CK2-mediated phosphorylation. This peptide has antineoplastic effect on lung cancer cells in vitro and vivo. To understand mechanisms involved such anticancer activity, NCI-H125 cell line proteomic profile after short-term incubation (45 min) with was investigated. As determined by 2-DE or 2D-LC-MS/MS, 137 proteins changed their abundances more than 2-fold response to treatment. The expression levels of related ribosome...
Protein kinase CK2 has emerged as an attractive therapeutic target in acute myeloid leukemia (AML), advent that becomes particularly relevant since the treatment of this hematological neoplasia remains challenging. Here we explored for first time effect clinical-grade peptide-based inhibitor CIGB-300 on AML cells proliferation and viability. internalization subcellular distribution were also studied, role B23/nucleophosmin 1 (NPM1), a major peptide solid tumors, was addressed by knock-down...
Subunit vaccines based on the receptor-binding domain (RBD) of spike protein SARS-CoV-2 provide one most promising strategies to fight COVID-19 pandemic. The detailed characterization primary structure by mass spectrometry (MS) is mandatory, as described in ICHQ6B guidelines. In this work, several recombinant RBD proteins produced five expression systems were characterized using a non-conventional protocol known in-solution buffer-free digestion (BFD). single ESI-MS spectrum, BFD allowed...