A5019411008- Advanced Proteomics Techniques and Applications
- Melanoma and MAPK Pathways
- vaccines and immunoinformatics approaches
- Pancreatic and Hepatic Oncology Research
- Cancer, Lipids, and Metabolism
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Mass Spectrometry Techniques and Applications
- Cutaneous Melanoma Detection and Management
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Bioinformatics and Genomic Networks
- Alzheimer's disease research and treatments
- Chemical Synthesis and Analysis
- Glycosylation and Glycoproteins Research
- Bacterial Infections and Vaccines
- Health, Environment, Cognitive Aging
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
- Biochemical and Molecular Research
- Caveolin-1 and cellular processes
- Monoclonal and Polyclonal Antibodies Research
Lund University
2019-2025
Skåne University Hospital
2021-2025
Centro de Ingeniería Genética y Biotecnología
2006-2023
Molecular Oncology (United States)
2023
Korea Research Institute of Chemical Technology
2023
Hungarian Research Network
2023
Universidad Nacional Autónoma de México
2017-2022
Universidad Autónoma del Estado de Morelos
2018
Instituto de Biología Funcional y Genómica
2017
National University of Quilmes
2009-2010
The hippocampus is important for memory formation and severely affected in the brain with Alzheimer disease (AD). Our understanding of early pathogenic processes occurring hippocampi AD limited due to tissue unavailability. Here, we report a chemical approach rapidly generate free-floating hippocampal spheroids (HSs), from human induced pluripotent stem cells. When used model AD, both APP atypical PS1 variant HSs displayed increased Aβ42/Aβ40 peptide ratios decreased synaptic protein levels,...
Prognostic analysis for early-stage (stage I/II) melanomas is of paramount importance customized surveillance and treatment plans. Since immune checkpoint inhibitors have recently been approved stage IIB IIC melanomas, prognostic tools to identify patients at high risk recurrence become even more critical. This study aims assess the effectiveness machine-learning algorithms in predicting melanoma using clinical histopathologic features from Electronic Health Records (EHRs). We collected 1720...
Melanoma is a highly heterogeneous disease, and deeper molecular classification essential for improving patient stratification treatment approaches. Here, we describe the histopathology-driven proteogenomic landscape of 142 treatment-naïve metastatic melanoma samples to uncover subtypes clinically relevant biomarkers. We performed an integrative analysis identify proteomic subtypes, assess impact BRAF V600 mutations, study profiles cellular composition tumor microenvironment. Clinical...
CIGB-300, formerly known as P15-tat, is a proapoptotic peptide with established antiproliferative activity in vitro and antitumoral vivo. This hypothesis-driven was initially selected for its ability to impair the CK2-mediated phosphorylation one of substrates through direct binding conserved acidic phosphoaceptor domain. However, actual vivo target(s) on human cancer cells among hundreds CK2 well subsequent events that lead apoptosis tumor remains be determined. In this work, we identified...
The molecular processes and proteomic markers leading to tumor progression (TP) in cervical cancer (CC) are either unknown or only partially understood. TP affects metabolic regulatory mechanisms that can be identified as changes. To identify which proteins differentially expressed understand the of progression, we analyzed dynamics proteome CC cell lines. This analysis revealed two up-regulated during TP, GSTM3 GSTP1. These involved maintenance, survival cellular stress response via NF-κB...
Melanoma of the skin is sixth most common type cancer in Europe and accounts for 3.4% all diagnosed cancers. More alarming degree recurrence that occurs with approximately 20% patients lethally relapsing following treatment. Malignant melanoma a highly aggressive metastases rapidly extend to regional lymph nodes (stage 3) distal organs 4). Targeted oncotherapy one standard treatment progressive stage 4 melanoma, BRAF inhibitors (e.g. vemurafenib, dabrafenib) combined MEK inhibitor...
Abstract Huntington's disease is a neurodegenerative disorder caused by CAG expansions in the huntingtin (HTT) gene. Modelling challenging, as rodent and cellular models poorly recapitulate seen ageing humans. To address this, we generated induced neurons through direct reprogramming of human skin fibroblasts, which retain age-dependent epigenetic characteristics. (HD-iNs) displayed profound deficits autophagy, characterized reduced transport late autophagic structures from neurites to soma....
Abstract The MM500 meta‐study aims to establish a knowledge basis of the tumor proteome serve as complement genome and transcriptome studies. Somatic mutations their effect on have been extensively characterized in melanoma. However, effects these genetic changes proteomic landscape impact cellular processes melanoma remain poorly understood. In this study, quantitative mass‐spectrometry‐based analysis is interfaced with pathological characterization, associated clinical data. landscape,...
Abstract The MM500 study is an initiative to map the protein levels in malignant melanoma tumor samples, focused on in‐depth histopathology coupled proteome characterization. and localization were determined for a broad spectrum of diverse, surgically isolated tumors originating from multiple body locations. More than 15,500 proteoforms identified by mass spectrometry, which chromosomal subcellular was annotated within both primary metastatic melanoma. data generated global proteomic...
Well-characterized archival formalin-fixed paraffin-embedded (FFPE) tissues are of much value for prospective biomarker discovery studies, and protocols that offer high throughput good reproducibility essential in proteomics. Therefore, we implemented efficient paraffin removal protein extraction from FFPE followed by an optimized two-enzyme digestion using suspension trapping (S-Trap). The protocol was then combined with TMTpro 16plex labeling applied to lung adenocarcinoma patient samples....
The Chromosome-Centric Human Proteome Project (C-HPP) is an international initiative. It aims to create a protein list expressed in human cells by each chromosomal and mitochondrial DNA enhance our understanding of disease mechanisms, akin the gene generated Genome Project. Transmembrane 160 (TMEM160) member transmembrane proteins (TMEM) family. TMEM have been implicated cancer-related processes, including cell proliferation, migration, epithelial-mesenchymal transition, metastasis,...
Sample Collection and Proteomic Analysis of Follicular Fluid
Using several melanoma proteomics data sets we created a single analysis platform that enables the discovery, knowledge build, and validation of diagnostic, predictive, prognostic biomarkers at protein level. Quantitative mass-spectrometry-based proteomic was obtained from five independent cohorts, including 489 tissue samples 394 patients with accompanying clinical metadata. We established an interactive R-based web comparison levels across diverse supports correlation between proteins...
CIGB-300 is a proapoptotic peptide-based drug that abrogates the CK2-mediated phosphorylation. This peptide has antineoplastic effect on lung cancer cells in vitro and vivo. To understand mechanisms involved such anticancer activity, NCI-H125 cell line proteomic profile after short-term incubation (45 min) with was investigated. As determined by 2-DE or 2D-LC-MS/MS, 137 proteins changed their abundances more than 2-fold response to treatment. The expression levels of related ribosome...
In comparison to other human cancer types, malignant melanoma exhibits the greatest amount of heterogeneity. After DNA-based detection BRAF V600E mutation in patients, targeted inhibitor treatment is current recommendation. This approach, however, does not take abundance therapeutic target, i.e., B-raf protein, into consideration. As shown by immunohistochemistry, protein expression profiles metastatic melanomas clearly reveal existence inter- and intra-tumor variability. Nevertheless,...
To date, several proteomics studies in cervical cancer (CC) have focused mainly on squamous (SCC). Our study aimed to discover and clarify differences SCC CAD that may provide valuable information for the identification of proteins involved tumor progression, CC as a whole, or specific CAD.Total protein extracts from 15 individual samples corresponding 5 different tissue types were compared with non-cancerous control group using bidimensional liquid chromatography-mass spectrometry (2D...
This work presents the results from a study of protein composition outer membrane vesicles VA-MENGOC-BC (Finlay Institute, Cuba), an available vaccine against serogroup B Neisseria meningitidis. Proteins were identified by means SCAPE, 2DE-free method for proteome studies. More than one hundred proteins detected tandem liquid chromatographymass spectrometry analysis fractions enriched in peptides devoid histidine or arginine residues, providing detailed description vaccine. A bioinformatic...