A5011071470- Advanced Proteomics Techniques and Applications
- Acute Myeloid Leukemia Research
- Machine Learning in Bioinformatics
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Vibrio bacteria research studies
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Protein purification and stability
- Entomological Studies and Ecology
- Chemical Synthesis and Analysis
- Insect Pheromone Research and Control
- Protein Degradation and Inhibitors
- Biomedical Text Mining and Ontologies
- Bioinformatics and Genomic Networks
- SARS-CoV-2 and COVID-19 Research
- Retinoids in leukemia and cellular processes
- Metabolomics and Mass Spectrometry Studies
- Growth Hormone and Insulin-like Growth Factors
- GDF15 and Related Biomarkers
- Influenza Virus Research Studies
- 14-3-3 protein interactions
- Immune Response and Inflammation
- Animal Virus Infections Studies
Centro de Ingeniería Genética y Biotecnología
2015-2025
National University of Quilmes
2010
Abstract Proteome-wide scale in a dose – and time-depending setting is crucial to fully understand the pharmacological mechanism of anticancer drugs as well identification candidates for drug response biomarkers. Here, we investigated effect CIGB-300 peptide at IC 50 80 doses during 1 4 h treatment on squamous lung cancer cell (NCI-H226) proteome. An overwhelming dose-dependent inhibitory with minor up-regulated proteins was observed by increasing level. Functional enrichment also common or...
The bacterial pathogen Vibrio cholerae requires colonizination of the human small intestine to cause cholera. anaerobic and slightly acidic conditions predominating there enhance toxicity low copper concentrations create a selective environment for bacteria with evolved detoxifying mechanisms. We reported previously that VCA0260, VCA0261 VC2216 gene products were synthesized only in V. grown microaerobiosis or anaerobiosis. Here we show ORFs VCA0260 are actually combined into single encoding...
Protein kinase CK2 has emerged as an attractive therapeutic target in acute myeloid leukemia (AML), advent that becomes particularly relevant since the treatment of this hematological neoplasia remains challenging. Here we explored for first time effect clinical-grade peptide-based inhibitor CIGB-300 on AML cells proliferation and viability. internalization subcellular distribution were also studied, role B23/nucleophosmin 1 (NPM1), a major peptide solid tumors, was addressed by knock-down...
CIGB-300 is a proapoptotic peptide-based drug that abrogates the CK2-mediated phosphorylation. This peptide has antineoplastic effect on lung cancer cells in vitro and vivo. To understand mechanisms involved such anticancer activity, NCI-H125 cell line proteomic profile after short-term incubation (45 min) with was investigated. As determined by 2-DE or 2D-LC-MS/MS, 137 proteins changed their abundances more than 2-fold response to treatment. The expression levels of related ribosome...
Coronaviruses constitute a global threat to the human population; therefore, effective pan-coronavirus antiviral drugs are required tackle future re-emerging virus outbreaks. Protein kinase CK2 has been suggested as promising therapeutic target in COVID-19 owing vitro activity observed after both pharmacologic and genetic inhibition of enzyme. Here, we explored putative effect anti-CK2 peptide CIGB-325 on bovine coronavirus (BCoV) infection using different viral infected cell-based assays....
Large cell lung carcinoma (LCLC) is one form of NSCLC that spreads more aggressively than some other forms, and it represents an unmet medical need. Here, we investigated for the first time effect anti-CK2 CIGB-300 peptide in NCI-H460 cells as LCLC model. were highly sensitive toward cytotoxicity, reaching a peak apoptosis at 6 h. Moreover, slightly impaired cycle cells. The interactomics profile revealed 300 proteins many them participated biological processes relevant cancer. Interrogation...
A method for quantitative proteomic analysis based on the selective isolation of multiply charged peptides (RH peptides) containing arginine and histidine residues is described. Two pools proteins are digested in tandem with lysyl-endopeptidase trypsin primary amino groups proteolytic separately labeled d3- d0-acetic anhydride. This reaction has a dual purpose: (i) to allow relative protein quantification two different conditions (ii) restrict positive charges presence histidine. The...
Casein kinase 2 (CK2) regulates a plethora of proteins with pivotal roles in solid and hematological neoplasia. Particularly, acute myeloid leukemia (AML) CK2 has been pointed as an attractive therapeutic target prognostic marker. Here, we explored the impact inhibition over phosphoproteome two cell lines representing major AML subtypes. Quantitative phosphoproteomic analysis was conducted to evaluate changes phosphorylation levels after incubation ATP-competitive inhibitor CX-4945....
Introduction The antinociceptive and pharmacological activities of C-Phycocyanin (C-PC) Phycocyanobilin (PCB) in the context inflammatory arthritis remain unexplored so far. In present study, we aimed to assess protective actions these compounds an experimental mice model that replicates key aspects human rheumatoid arthritis. Methods Antigen-induced (AIA) was established by intradermal injection methylated bovine serum albumin C57BL/6 mice, one hour before antigen challenge, either C-PC (2,...
CIGB-552 is a cell-penetrating peptide that exerts in vitro and vivo antitumor effect on cancer cells. In the present work, mechanism involved such anticancer activity was studied using chemical proteomics expression-based culture cell lines. interacts with at least 55 proteins, as determined by proteomics. A temporal differential based iTRAQ quantification method performed to identify modulated proteins. The proteomic profile includes 72 differentially expressed proteins response treatment....
Abstract Protein kinase CK2 has emerged as an attractive therapeutic target in acute myeloid leukemia (AML), advent that becomes particularly relevant since the treatment of this hematological neoplasia remains challenging. Here we explored for first time effect clinical-grade peptide-based inhibitor CIGB-300 on AML cells proliferation and viability. internalization subcellular distribution were also studied, role B23/nucleophosmin 1 (NPM1), a major peptide solid tumors, was addressed by...
Protein kinase CK2 is a highly pleiotropic and ubiquitously expressed Ser/Thr with instrumental roles in normal pathological states, including neoplastic phenotype solid tumor hematological malignancies. In line previous reports, has been suggested as an attractive prognostic marker molecular target acute myeloid leukemia (AML), blood malignant disorder that remains unmet medical need. Accordingly, this work investigates the complex landscape of cellular perturbations supporting antileukemic...
Abstract Casein kinase 2 (CK2) regulates a plethora of proteins with pivotal roles in solid and hematological neoplasia. Particularly, acute myeloid leukemia (AML) CK2 has been pointed as an attractive therapeutic target prognostic marker. Here, we explored the impact inhibition over phosphoproteome two cell lines representing major AML subtypes. Quantitative phosphoproteomic analysis was conducted to evaluate changes phosphorylation levels after incubation ATP-competitive inhibitor CX-4945....
Jusvinza is an immunomodulatory drug composed of altered peptide ligand (APL) designed from a novel CD4+ T cell epitope human heat shock protein 60 (HSP60), autoantigen involved in the pathogenesis rheumatoid arthritis (RA). The induces regulatory cells and decreases levels TNF-α IL-17; pre-clinical phase I clinical studies support its use for treatment RA. This was repositioned COVID-19 patients with signs hyperinflammation. Neutrophils play pathogenic role both RA severe forms COVID-19. To...
Proteome-wide scale in a dose- and time-depending setting is crucial drug-regulated proteomics to fully understand the pharmacological mechanism of anticancer drugs as well identification proteins suitable for drug response biomarkers. Here, we investigated effect CIGB-300 peptide at IC50 IC80 dose levels during 1 4 hours treatment on squamous lung cancer cell (NCI-H226) proteome. A dose-dependent inhibitory global while slight change upregulated was observed, by increasing level. The...
Abstract SOBERANA ® 02 is a safe and effective conjugate vaccine against SARS-CoV-2, produced using the maleimide-thiol chemistry. In this vaccine, Cys 538 in recombinant receptor binding domain (RBD) of SARS-CoV-2 linked, through thiosuccinimide linker, to lysine residues tetanus toxoid (TT) preparation. LC-MS/MS analysis revealed that TT complex mixture proteins, similar other TTs where detoxified neurotoxin (d-TeNT) has been shown be most abundant protein (30-56%), regardless...
Sample preparation and protein fractionation are important issues for proteomic studies. Protein extraction procedures strongly affect the performance of methods by provoking dispersion in several fractions. The most notable exception is gel-based electrophoretic due to its resolution effectiveness sodium dodecyl sulfate as a solubilizing agent, while main limitation lies poor recovery gel-trapped proteins. We created fractionator device separate complex mixture proteins peptides that based...
Abstract Sample preparation and protein fractionation are important issues in proteomic studies spite of the technological achievements on mass spectrometry. Protein extraction procedures strongly affect performance methods by provoking dispersion several fractions. The most notable exception is SDS-PAGE-based due to its extraordinary resolution effectiveness SDS as a solubilizing agent. Its main limitation lies poor recovery gel-trapped proteins, where electro-elution successful approach...