A5067729934- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- vaccines and immunoinformatics approaches
- Research on Leishmaniasis Studies
- Analytical Chemistry and Chromatography
- Peptidase Inhibition and Analysis
- Trypanosoma species research and implications
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Enzyme Structure and Function
- Marine Invertebrate Physiology and Ecology
- Protein purification and stability
- Immunotherapy and Immune Responses
- Bacterial Infections and Vaccines
- Heat shock proteins research
- Marine Sponges and Natural Products
- Rheumatoid Arthritis Research and Therapies
- Toxin Mechanisms and Immunotoxins
- Bacterial Genetics and Biotechnology
- Biochemical and Molecular Research
- Neurobiology and Insect Physiology Research
- Genomics and Phylogenetic Studies
Centro de Ingeniería Genética y Biotecnología
2013-2024
Fundação Oswaldo Cruz
2016-2022
ORCID
2022
National University of Quilmes
2010
National Center for Genetic Engineering and Biotechnology
1995-2003
Osaka University
1992
Protein Research Foundation
1992
Abstract In this study we concentrate on replacing side chains as a subtask of model building by homology. Two problems arise. How to determine potential low energy rotamers? And how avoid the combinatorial explosion that results from combination many residues for which multiple good rotamers are predicted? We attempt solve these choosing position‐specific rather than generalized and sorting have be modelled function their freedom in rotamer space. The practical advantages our method quality...
SeqMS, a software aid for de novo sequencing by tandem mass spectrometry (MS/MS), which was initially developed the automated interpretation of high-energy collision-induced dissociation (CID) MS/MS spectra peptides, has been applied to low-energy CID and post-source decay (PSD) peptides. Based on peptide backbone fragmented ions their related ions, are dominant observed in latter two techniques, types propensities be have optimized efficient spectra. In typical example, modified SeqMS...
Induction of immune tolerance as therapeutic approach for autoimmune diseases constitutes a current research focal point. In this sense, we aimed to evaluate an altered peptide ligand (APL) induction peripheral in patients with rheumatoid arthritis (RA). A novel T-cell epitope from human heat-shock protein 60 (Hsp60), autoantigen involved the pathogenesis RA, was identified by bioinformatics tools and APL design starting epitope. We investigated ability inducing regulatory T cells (Treg...
Internal rearrangement involving the loss of C-terminal amino acid residue upon collision-induced dissociation (CID) or metastable decomposition was studied for protonated peptides. To investigate structural characteristics peptides responsible this rearrangement, a series synthetic were prepared and subjected to B/E-linked scan tandem mass spectrometric analyses using four-sector instrument. The results showed that position basic in peptide sequence its basicity have significant influence...
Detrimental effects of malnutrition on immune responses to pathogens have long been recognized and it is considered a main risk factor for various infectious diseases, including visceral leishmaniasis (VL). Thymus target both infection, but its role in the response Leishmania infantum malnourished individuals barely studied. Because we previously observed thymic atrophy significant reduction cellularity chemokine levels mice infected with L. infantum, postulated that microenvironment...
SeqMS, a software program designed for the automated interpretation of high-energy collision-induced dissociation (CID) mass spectra singly protonated peptides ionized by fast atom bombardment, has been developed. The is capable probing sequence an unknown peptide, and even certain modified peptides. program, compiled WINDOWS95 or NT, also permits retrieval raw data reconstruction on user-friendly graphical interface with aid several tools processing spectra, which include setting multiple...
CIGB-300 is a proapoptotic peptide-based drug that abrogates the CK2-mediated phosphorylation. This peptide has antineoplastic effect on lung cancer cells in vitro and vivo. To understand mechanisms involved such anticancer activity, NCI-H125 cell line proteomic profile after short-term incubation (45 min) with was investigated. As determined by 2-DE or 2D-LC-MS/MS, 137 proteins changed their abundances more than 2-fold response to treatment. The expression levels of related ribosome...
Rheumatoid arthritis is a systemic autoimmune disease mediated by T cells. Productive engagement of cell receptors major histocompatibility complex-peptide leads to proliferation, differentiation and the definition effector functions. Altered peptide ligands (APL) generated amino acid substitutions in antigenic have diverse effects on response. We predicted novel epitope from human heat-shock protein 60, an autoantigen involved pathogenesis rheumatoid arthritis. Three APLs were designed this...
Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic relapsing-remitting joint inflammation. Perturbations in the balance between CD4 + T cells producing IL-17 and CD25(high)FoxP3 Tregs correlate with irreversible bone cartilage destruction RA. APL1 an altered peptide ligand derived from CD4+ T-cell epitope of human HSP60, autoantigen expressed inflamed synovium, which increases frequency CD25(high)FoxP3+ peripheral blood mononuclear RA patients. The aim this...
Aerobic metabolism generates reactive oxygen species that may cause critical harm to the cell. The aim of this study is characterization stress responses in model aromatic-degrading bacterium Paraburkholderia xenovorans LB400 oxidizing agents paraquat and H2O2.Antioxidant genes were identified by bioinformatic methods genome P. LB400, phylogeny its OxyR SoxR transcriptional regulators studied. Functionality from strain was assessed complementation with null mutant aeruginosa ΔsoxR,...
A gene encoding a multiepitope polypeptide (MEP) has been synthesized. It contains the information for (1) an 11-amino acid (aa) epitope from C1 region of gp120 HIV-1 and (2) 3 epitopes 15 amino acids each, central part V3 loop isolates MN, SC, WMJII. These four segments are linked by short spacer peptide AGGGA. This was cloned in plasmid vector expressed Escherichia coli as fusion product with 62-aa fragment human IL-2. The recombinant protein TAB1 purified washed pellet procedures...
Mass spectrometry is now firmly established as a powerful technique for the identification and characterization of proteins when used in conjunction with sequence databases. Various approaches involving stable-isotope labeling have been developed quantitative comparisons between paired samples proteomic expression analysis by mass spectrometry. However, interpretation such spectra far from being fully automated, mainly due to difficulty analyzing complex patterns resulting overlap multiple...
This work presents the results from a study of protein composition outer membrane vesicles VA-MENGOC-BC (Finlay Institute, Cuba), an available vaccine against serogroup B Neisseria meningitidis. Proteins were identified by means SCAPE, 2DE-free method for proteome studies. More than one hundred proteins detected tandem liquid chromatographymass spectrometry analysis fractions enriched in peptides devoid histidine or arginine residues, providing detailed description vaccine. A bioinformatic...