A5032495744- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- Radiopharmaceutical Chemistry and Applications
- Infant Nutrition and Health
- Glycosylation and Glycoproteins Research
- Zoonotic diseases and public health
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- Infective Endocarditis Diagnosis and Management
- Viral Infections and Vectors
- Leptospirosis research and findings
National University of San Marcos
2022
National Institute of Quality
2022
National Center for Genetic Engineering and Biotechnology
2009-2015
Centro de Ingeniería Genética y Biotecnología
1995-2014
This work presents the results from a study of protein composition outer membrane vesicles VA-MENGOC-BC (Finlay Institute, Cuba), an available vaccine against serogroup B Neisseria meningitidis. Proteins were identified by means SCAPE, 2DE-free method for proteome studies. More than one hundred proteins detected tandem liquid chromatographymass spectrometry analysis fractions enriched in peptides devoid histidine or arginine residues, providing detailed description vaccine. A bioinformatic...
Neisseria meningitidis serogroup C polysaccharide (CCPS) was conjugated to the carrier protein P64k using two different conjugation procedures, condensation mediated by carbodiimide with adipic acid dihydrazide as spacer and reductive amination method. BALB/c mice were immunized resultant polysaccharide-protein conjugates immune response evaluated. All assayed generated at least 10-fold higher antibody titers than free polysaccharide. The method rendered best conjugate (CCPS-P64kR) that able...
P64k is a meningococcal protein from Neisseria meningitidis that has been obtained by recombinant DNA technology. Recombinant extensively characterized physicochemical and immunological methods. Lately this found to act as versatile carrier for weak antigens in mice. In the present work, Phase I clinical trial was carried out healthy volunteers who received three inoculations of either placebo or (20 50 μ g). No severe adverse events occurred during trial. Only mild ten were observed. At 1...
In the post‐genomic era, every aspect of production proteins must be accelerated. this way, several vectors are currently exploited for rapid recombinant in Escherichia coli . N‐terminal fusions to first 47 amino acids LpdA (dihydrolipoamide dehydrogenase A) protein Neisseria meningitidis have been shown increase expression proteins. Consequently, we constructed a modified fusion vector, introducing blue/white colony selection by exploiting bicistronic gene organization. new sequence...
Leptospirosis is a zoonotic disease caused by the genus Leptospira, presenting complex and dynamic epidemiology. To determine genetic variability its phylogenetic relationship of Leptospira spp isolates from three sources in Iquitos (Peruvian Amazon) 2002 to 2013, seven MLST genes were analyzed obtain Sequence Type (ST) these sequences concatenated for analysis. The between STs was determined with goeBURST algorithm diversity using DnaSP. Of 51 isolates, 48 pathogenic belonging five...
P64k is a minor outer membrane protein from Neisseria meningitidis. This has been produced at high levels in Escherichia coli. We generated group of monoclonal antibodies (mAbs) against recombinant P64k, which recognise four non-overlapping epitopes, as shown using competition assays with biotinylated mAbs. The sequences involved mAbs binding were mapped synthetic overlapping peptides derived the protein, and located previously determined three-dimensional structure protein. These also...
Pertussis is a vaccine preventable disease caused by the Gram-negative bacterium Bordetella pertussis ( B. ). Acellular vaccines as regard to whole cell are less reactogenic and very similar in efficacy. An increased incidence of infections countries where acellular widely used has questioned their effectiveness. In fact, circulating strains differ from due variations protein sequences associated evolutionary process . Furthermore, antigens change throughout manufacturing chemical treatments...
Abstract The 5C outer membrane protein, one of the N. meningitidis class 5 proteins, was preferably expressed in bacteria isolated from nasopharynx and its role adhering to mucosal cells invading them as well development anti‐5C antibodies healthy carriers demonstrated. Anti‐5C monoclonal are bactericidal presence human complement. immunodominant region protein is highly conserved among different strains , opc gene, which encodes does not seem show antigenic variations. Here isolation gene...
Abstract The gene coding for the class 1 outer membrane protein from Neisseria meningitidis strain B385 (B : 4 P1.15) was isolated by Polymerase Chain Reaction (PCR) and cloned into Sma I cut M13mp 18 vector. Then, a Xba restriction site created using an oligonucleotide‐directed in vitro mutagenesis system at start of fragment. In order to express protein, this fragment fused 180 base pairs corresponding 60 amino acids N terminus interleukin‐2 under control tryptophan promoter (Ptrp)....