A5020083621- Advanced Proteomics Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Blood properties and coagulation
- Adipose Tissue and Metabolism
- Parkinson's Disease Mechanisms and Treatments
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Botulinum Toxin and Related Neurological Disorders
- Protein Interaction Studies and Fluorescence Analysis
- Mass Spectrometry Techniques and Applications
- Machine Learning in Bioinformatics
- Microfluidic and Bio-sensing Technologies
- Sirtuins and Resveratrol in Medicine
- DNA Repair Mechanisms
- Metal Extraction and Bioleaching
- Polymer Surface Interaction Studies
- Advanced Biosensing Techniques and Applications
- Skin and Cellular Biology Research
- Acute Lymphoblastic Leukemia research
- Fungal and yeast genetics research
- Bacterial biofilms and quorum sensing
- Histone Deacetylase Inhibitors Research
- Machine Learning in Materials Science
- RNA regulation and disease
- Telomeres, Telomerase, and Senescence
École Polytechnique Fédérale de Lausanne
2013-2024
University of Lausanne
2017
University Hospital of Geneva
2000-2001
Increasing evidence suggests that phosphorylation may play an important role in the oligomerization, fibrillogenesis, Lewy body (LB) formation, and neurotoxicity of α-synuclein (α-syn) Parkinson disease. Herein we demonstrate α-syn is phosphorylated at S87 vivo within LBs. The levels S87-P are increased brains transgenic (TG) models synucleinopathies human from Alzheimer disease (AD), LB (LBD), multiple system atrophy (MSA) patients. Using antibodies against (S129-P S87-P), a significant...
The two major isoforms of the oncogenic Bcr–Abl tyrosine kinase, p210 and p190, are expressed upon Philadelphia chromosome translocation. is hallmark chronic myelogenous leukemia, whereas p190 occurs in majority B-cell acute lymphoblastic leukemia. Differences protein interactions activated signaling pathways that may be associated with different diseases driven by unknown. We have performed a quantitative comparative proteomics study p190. Strong differences interactome phosphoproteome were...
A number of two-dimensional electrophoresis (2-DE) reference maps from mouse samples have been established and could be accessed through the internet. An up-to- date list can found in WORLD-2D PAGE (http://www.expasy.ch/ch2d/2d-index.html), an index 2-DE databases services. None them were white brown adipose tissues, pancreatic islets, liver nuclei skeletal muscle. This publication describes SWISS-2D database. Proteins present (C57Bl/6J) liver, nuclei, muscle, tissue islets are assembled...
Relative quantitation is key to enable differential proteomics and hence answer biological questions by comparing samples. Classical approaches involve stable isotope labeling with/without spiked standards. Although isotopes may lead precise results, their application not straightforward. In Proteomics, 2004, 4, 2333-2351, we proposed an approach where summed peptide identification scores derive a semiquantitative abundance indicator. this study, combine such indicator with statistical test...
Nanoparticles (NPs) in contact with a biological environment get covered by proteins and some are loosely bound tightly bound. The latter form hard protein corona (HPC) which is known to determine their behavior. Therefore, order study the behaviour of NPs one needs start from HPC. However, established methods standards HPC isolation still not known. This especially challenge case magnetic major branch nanomedicine. we developed novel method, multi-step centrifugation method (MSCM), for...
We present an integrated proteomics platform designed for performing differential analyses. Since reproducible results are essential comparative studies, we explain how improved reproducibility at every step of our laboratory processes, e.g. by taking advantage the powerful information management system developed. The capacity is validated detecting known markers in a real sample and spiking experiment. introduce innovative two-dimensional (2-D) plot displaying identification combined with...
Tissue transglutaminase (tTG) has been implicated in the pathogenesis of Parkinson disease (PD). However, exactly how tTG modulates structural and functional properties α-synuclein (α-syn) contributes to PD remains unknown. Using site-directed mutagenesis combined with detailed biophysical mass spectrometry analyses, we sought identify exact residues involved tTG-catalyzed cross-linking wild-type α-syn mutants associated PD. To better understand consequences each reaction, determined effect...
In biological environments, the surface of nanoparticles (NPs) are modified by protein corona (PC) that determines their behavior. Unfortunately, in vitro tests still give different PC than vivo causing vitro–in discrepancy; hence, studies not indicative for NPs' behavior vivo. Here is demonstrated strongly influenced type extracellular fluid (ECF), blood or lymph, high and low flow conditions transitions between ECFs, a combination these parameters. As result, this study approaches fluidic...
We decoupled electron-transfer dissociation (ETD) and collision-induced of charge-reduced species (CRCID) events to probe the lifetimes intermediate radical in ETD-based ion trap tandem mass spectrometry peptides. Short-lived intermediates formed upon electron transfer require less energy for product formation appear regular ETD spectra, whereas long-lived additional vibrational yield ions as a function CRCID amplitude. The observed dependencies complement results obtained by...
Mycobacterium tuberculosis has a remarkable ability to persist within the human host as clinically inapparent or chronically active infection. Fatty acids are thought be an important carbon source used by bacteria during long term Catabolism of fatty requires reprogramming metabolic networks, and enzymes central this have been targeted for drug discovery. smegmatis, nonpathogenic relative M. tuberculosis, is often model system because similarity basic cellular processes in these two species....
While it is well established that the surface of a nanoparticle plays pivotal role for protein corona, vast number proteins present in biological media render general conclusions about affinities between surfaces and nontrivial. Recently published articles increasingly reveal differences systems an ever increasing influencing factors corona. In contrast, study posits reported may, at least part, be due to poor experimental design, which leads biased results. The investigates adsorption onto...
Tissue transglutaminase (TGase) has been implicated in a number of cellular processes and disease states, where the enzymatic actions TGase may serve both, cell survival apoptosis. To date, precise functional properties or death mechanisms still remain elusive. TGase-mediated cross-linking reported to account for formation insoluble lesions conformational diseases. We report here that induces intramolecular β-amyloid peptide (Aβ), resulting structural changes monomeric Aβ. Using high...
Mitogen-activated protein kinase (MAPK) pathways constitute key regulatory elements linking extracellular stimuli to nuclear gene expression. Immediate-early responsive genes (IEGs) of the activator 1 (AP-1) family, such as fos, achieve peak expression levels shortly after cells are stimulated with growth factors and sharply decrease thereafter. Several AU-rich binding proteins (AUBPs), including HuR (Hu-antigen R, Elav-like 1, ELAVL1) KSRP (far upstream element-binding 2, KHSRP) bind a fos...
Desulfotomaculum reducens strain MI-1 is a Gram-positive, sulfate-reducing bacterium also capable of reducing Fe(III). Metal reduction in Gram-positive bacteria poorly understood. Here, we investigated Fe(III) with lactate, non-fermentable substrate, as the electron donor. Lactate consumption concomitant to reduction, but does not support significant growth, suggesting that little energy can be conserved from this process and it may occur fortuitously. D. reduce both soluble...
This short communication describes the establishment of a two-dimensional electrophoresis (2-DE) reference map nuclear proteins isolated from human liver. The liver nuclei 2-DE contains 1497 spots. In an initial identification study using peptide mass fingerprinting as means protein we were able to identify 26 spots corresponding 15 different proteins. is now included in SWISS-2DPAGE database, which can be accessed through ExPASy server (http://www.expasy.ch/ch2d/).
Human fetal progenitor tenocytes (hFPT) produced in defined cell bank systems have recently been characterized and qualified as potential therapeutic sources tendon regenerative medicine. In view of further developing the manufacture processes such cell-based active pharmaceutical ingredients (API), effects hypoxic vitro culture expansion on key cellular characteristics or process parameters were evaluated. To this end, multiple aspects comparatively assessed normoxic incubation (i.e., 5%...
Serine proteases are important environmental contributors of enterovirus biocontrol. However, the structural features molecular interaction accounting for susceptibility enteroviruses to remains unexplained. Here, we describe mechanisms involved in recruitment serine viral capsids. Among virus types used, coxsackievirus A9 (CVA9), but not CVB5 and echovirus 11 (E11), was inactivated by Subtilisin A a host-independent manner, while Bovine Pancreatic Trypsin (BPT) only reduced CVA9 infectivity...
Meiotic progression in S. pombe is regulated by stage-specific gene expression and translation, changes RNA stability, of anti-sense transcripts, targeted proteolysis regulatory proteins. We have used SILAC labeling to examine the relative levels proteins diploid cells during meiosis. Among 3,268 quantified at all time points, 880 changed least 2-fold; majority showed stepwise increases or decreases meiotic divisions, while some transiently. Overall, we observed reductions involved anabolism...
Summary Enteroviruses are human pathogens known to challenge water safety 1,2 . Among the microbial stressors found in water, bacterial serine proteases contribute control of enterovirus persistence 3 However, structural interactions accounting for susceptibility enteroviruses remains unexplained. Here, we describe molecular mechanisms involved recruitment viral capsids. virus types used, coxsackievirus A9 (CVA9), but not CVB5 and echovirus 11 (E11), was inactivated by Subtilisin A a...