A5060442899- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Parkinson's Disease Mechanisms and Treatments
- Antimicrobial Peptides and Activities
- Blood properties and coagulation
- Alzheimer's disease research and treatments
- Lysosomal Storage Disorders Research
- Insect and Arachnid Ecology and Behavior
- Malaria Research and Control
- Protein Structure and Dynamics
- Neurological disorders and treatments
- Tuberculosis Research and Epidemiology
- Botulinum Toxin and Related Neurological Disorders
- Invertebrate Immune Response Mechanisms
- Lipid Membrane Structure and Behavior
- Biochemical and Structural Characterization
- Protease and Inhibitor Mechanisms
- Crustacean biology and ecology
- Cell Adhesion Molecules Research
- Research on Leishmaniasis Studies
- Nanoparticle-Based Drug Delivery
- Catalytic Alkyne Reactions
- Genomics and Phylogenetic Studies
- Adipose Tissue and Metabolism
- Neuroscience and Neuropharmacology Research
École Polytechnique Fédérale de Lausanne
2012-2022
University of Lausanne
2017
Zero to Three
2014
Muscular Dystrophy Canada
2002
Centre National de la Recherche Scientifique
1994-1999
Institut de Biologie Moléculaire et Cellulaire
1998
Institut de Chimie
1997
Inserm
1997
Institut Pasteur
1997
Institut de Chimie de Strasbourg
1997
Increasing evidence suggests that phosphorylation may play an important role in the oligomerization, fibrillogenesis, Lewy body (LB) formation, and neurotoxicity of α-synuclein (α-syn) Parkinson disease. Herein we demonstrate α-syn is phosphorylated at S87 vivo within LBs. The levels S87-P are increased brains transgenic (TG) models synucleinopathies human from Alzheimer disease (AD), LB (LBD), multiple system atrophy (MSA) patients. Using antibodies against (S129-P S87-P), a significant...
It is known that the nanoparticle-cell interaction strongly depends on physicochemical properties of investigated particles. In addition, medium density and viscosity influence colloidal behaviour nanoparticles. Here, we show how nanoparticle-protein interactions are related to particular characteristics particles, such as their stability, this significantly influences subsequent in vitro. Therefore, different surface charged superparamagnetic iron oxide nanoparticles were synthesized...
The two major isoforms of the oncogenic Bcr–Abl tyrosine kinase, p210 and p190, are expressed upon Philadelphia chromosome translocation. is hallmark chronic myelogenous leukemia, whereas p190 occurs in majority B-cell acute lymphoblastic leukemia. Differences protein interactions activated signaling pathways that may be associated with different diseases driven by unknown. We have performed a quantitative comparative proteomics study p190. Strong differences interactome phosphoproteome were...
Significance Our experiments provide the analysis of lipid metabolite circadian oscillations in a cellular system synchronized vitro, suggesting cell-autonomous diurnal changes profiles independent feeding. Moreover, our work represents comprehensive comparison between composition human skeletal muscle derived from sedentary healthy adults, receiving hourly isocaloric solutions, and primary myotubes cultured vitro. A substantial number metabolites, particular membrane lipids, exhibited...
We have developed an approach based on a differential mass spectrometric analysis to detect molecules induced during the immune response of Drosophila , regardless their biological activities. For this, we applied directly matrix-assisted laser desorption/ionization MS hemolymph samples from individual flies before and after challenge. This method provided precise information molecular masses immune-induced allowed detection, in range 1.5–11 kDa, 24 (DIMs). These are all peptides, four...
Human tuberculosis is caused by the intracellular pathogen Mycobacterium tuberculosis. Sequencing of genome M. strain H37Rv has predicted 3924 open reading frames, and enabled identification proteins from this bacterium peptide mass fingerprinting. Extracellular culture medium in cellular extracts were examined two-dimensional gel electrophoresis using immobilized pH gradient technology. By spectrometry immunodetection, 49 filtrate 118 lysate identified, 83 which novel. To date, 288 have...
Two glycoforms (AH1 and AH2) of androgenic hormone, its corresponding hormone precursor derived from HPLC-purified gland extract the woodlouse Armadillidium vulgare were fully characterized by microsequencing mass spectrometry. The amino-acid sequences two identical; they consist peptide chains, A B, 29 44 amino acids, respectively, with chain carrying one N-glycosylated moiety on Asn18. chains are linked disulfide bridges. Glycoforms only differentiated size heterogeneity glycan chain....
Recently, we have isolated from bovine chromaffin granules and identified two natural peptides possessing antibacterial activity: secretolytin (chromogranin B 614-626) enkelytin (proenkephalin-A 209-237). Here, characterize a large fragment, corresponding to chromogranin A 79-431, that inhibits growth of both Gram-positive Gram-negative bacteria. The aim the present work was determine shortest active peptide located in 79-431 region. Three peptides, which shared same 173-194 sequence...
Relative quantitation is key to enable differential proteomics and hence answer biological questions by comparing samples. Classical approaches involve stable isotope labeling with/without spiked standards. Although isotopes may lead precise results, their application not straightforward. In Proteomics, 2004, 4, 2333-2351, we proposed an approach where summed peptide identification scores derive a semiquantitative abundance indicator. this study, combine such indicator with statistical test...
Merozoites of malaria parasites invade red blood cells (RBCs), where they multiply by schizogony, undergoing development through ring, trophozoite and schizont stages that are responsible for pathogenesis. Here, we report a protein kinase-mediated signalling pathway involving host RBC PAK1 MEK1, which do not have orthologues in the Plasmodium kinome, is selectively stimulated falciparum-infected (versus uninfected) RBCs, as determined use phospho-specific antibodies directed against...
α-Synuclein is the major component of Lewy bodies and a candidate biomarker for neurodegenerative diseases in which are common, including Parkinson's disease dementia with bodies. A large body literature suggests that these disorders characterized by reduced concentrations α-synuclein cerebrospinal fluid (CSF), overlapping compared to healthy controls variability across studies. Several reasons can account this variability, technical ones, such as inter-assay inter-laboratory variation...
A prototype matrix-assisted laser desorption/ionization quadrupole time-of-flight (MALDI-TOF) tandem mass spectrometer was used to sequence a series of phosphotyrosine-, phosphothreonine- and phosphoserine-containing peptides. The high resolution accuracy the instrument allowed localization one, three or four phosphorylated amino acid residues in phosphopeptides up 3.1 kDa. Tandem spectra two different phosphotyrosine peptides permitted determination one phosphorylation sites, respectively....
Aerolysin, a virulence factor secreted by Aeromonas hydrophila , is representative of group β‐sheet toxins that must form stable homooligomers in order to be able insert into biological membranes and generate channels. Electron microscopy image analysis two‐dimensional membrane crystals had previously revealed structure with 7‐fold symmetry et al. (1992) EMBO J. 11, 2457–2463]. However, this unusual molecularity the channel remained confirmed an independent method since low‐resolution...
Formation of the 4‐kDa peptides, which are essential constituents extracellular plaques in Alzheimer's disease, involves sequential cleavage amyloid precursor protein (APP) by β‐ and γ‐secretases. The carboxy‐terminal 99‐amino‐acid peptide is liberated from APP β‐secretase was used as a potential native substrate γ‐secretase(s). With addition an initiator Met FLAG sequence at C‐terminus (β5APP100‐FLAG), it expressed Escherichia coli under control T7 promoter. preferred site(s) N‐terminal...
The chromaffin granules have been shown to be an excellent model study the processing of proenkephalin‐A and chromogranins. Recently, we reported a dealing with chromogranin B/secretogranin I occurrence C‐terminal B‐derived peptide 614–626 which was antibacterial activity [Strub, J. M., Garcia‐Sablone, P., Looning, K., Taupenol, L., Hubert, Van Dorsselaer, A., Aunis, D. & Metz‐Boutigue, M. H. (1995) Eur. Biochem. 229 , 356–368], We also observed that this new present in associated other...
Nanoparticles (NPs) in contact with a biological environment get covered by proteins and some are loosely bound tightly bound. The latter form hard protein corona (HPC) which is known to determine their behavior. Therefore, order study the behaviour of NPs one needs start from HPC. However, established methods standards HPC isolation still not known. This especially challenge case magnetic major branch nanomedicine. we developed novel method, multi-step centrifugation method (MSCM), for...
Most cells acquire cholesterol by endocytosis of circulating low-density lipoproteins (LDLs). After cholesteryl ester de-esterification in endosomes, free is redistributed to intracellular membranes via unclear mechanisms. Our previous work suggested that the unconventional phospholipid lysobisphosphatidic acid (LBPA) may play a role modulating flux through endosomes. In this study, we used Prestwick library FDA-approved compounds high-content, image-based screen endosomal lipids, and...
In specialized cell types, lysosome-related organelles support regulated secretory pathways, whereas in nonspecialized cells, lysosomes can undergo fusion with the plasma membrane response to a transient rise cytosolic calcium. Recent evidence also indicates that lysosome secretion be controlled transcriptionally and promote clearance storage diseases. addition, is accumulating low concentrations of cyclodextrins reduce cholesterol-storage phenotype cells animals cholesterol disease...
Neutrophil elastase (NE) and cathepsin G are two serine proteinases released concomitantly by stimulated polymorphonuclear neutrophils. We previously demonstrated that while NE itself does not activate human platelets, it strongly enhances the weak aggregation induced a threshold concentration of (threshold G) (Renesto, P., Chignard, M. (1993) Blood 82, 139-144). The aim this study was to delineate molecular mechanisms involved in potentiation process. Two main pieces data prompted us focus...
We present an integrated proteomics platform designed for performing differential analyses. Since reproducible results are essential comparative studies, we explain how improved reproducibility at every step of our laboratory processes, e.g. by taking advantage the powerful information management system developed. The capacity is validated detecting known markers in a real sample and spiking experiment. introduce innovative two-dimensional (2-D) plot displaying identification combined with...