Niacinamide (or nicotinamide) is a small-molecule hydrosoluble vitamin with essential metabolic functions in mammalian cells. has become key functional ingredient diverse skincare products and cosmetics. This plays pivotal role NAD+ synthesis, notably contributing to redox reactions energy production cutaneous Via diversified biochemical mechanisms, niacinamide also known influence human DNA repair cellular stress responses. Based on decades of safe use cosmetics, recently gained widespread...

Providing accurate and up-to-date practical tools enabling oversight of platelet-rich plasma (PRP) legislation the appropriate standards to be implemented for its manufacture use in Europe is a demanding task. This due rapid medico-technological advancements, slowness disparity updates enforcement between member states, many reported gray-zone practices, notably autologous PRP use. The levels risk associated with blood manipulation processes generally dictate manufacturing requirements...

Wound healing in fetal skin is characterized by the absence of scar tissue formation, which not dependent on intrauterine environment and amniotic fluid. Fetal cells have capacity extraordinary expansion we describe herein development a cell bank where from one organ donation (2–4 cm2) it possible to produce several hundred million constructs 9 × 12 cm2. grow three four times more rapidly than older cultured same manner these banked are very resistant against physical oxidative stress when...

Abstract Chronic and acute tendon injuries are frequent afflictions, for which treatment is often long unsatisfactory. When facing extended injuries, matrices scaffolds with sufficient biomechanical properties required surgical repair could additionally serve as supports cellular therapies to improve healing. In this study, protocols of either commonly used detergents only (SDS 1%, Triton TBP Tween‐20 1%) or a combination freeze/thaw (F/T) cycles decellularization agents (NaCl 1M, ddH 2 O)...

Tissue decellularisation has gained much attention in regenerative medicine as an alternative to synthetic materials. In decellularised tissues, biological cues can be maintained and provide cellular environments still unmet by Supercritical CO2 (scCO2 ) recently emerged a promising technique aggressive detergents; addition, scCO2 provides innate sterilisation. However, date, with is limited only few tissue types low density. the current study, decellularise high density including articular...

Chlorhexidine is known to be a potent antiseptic with evidence of beneficial role in burn care. Nevertheless, several vitro studies have reported cytotoxicity on cultured cells, while vivo and clinical data seem show more controversial results. In the frame this work, we aimed evaluate use chlorhexidine units worldwide sending survey professionals field. We associated results those perspectives literature update recommendations for specific protocols management. The showed that there no...

Current restrictions for human cell-based therapies have been related to technological limitations with regards cellular proliferation capacity (simple culture conditions), maintenance of differentiated phenotype primary cell and transmission communicable diseases. Cultured fetal cells from one organ donation could possibly meet the exigent stringent technical aspects development therapeutic products. Master working banks (skin) can be developed in short periods time safety tests performed...

Platelet-rich plasma (PRP) preparations have recently become widely available in sports medicine, facilitating their use regenerative therapy for ligament and tendon affections. Quality-oriented regulatory constraints PRP manufacturing clinical experiences underlined the critical importance of process-based standardization, a pre-requisite sound homogeneous efficacy evaluation. This retrospective study (2013-2020) considered standardized GMP medicine-related autologous tendinopathies at...

Hyaluronic acid (HA) hydrogels are commonly used for facial dermal filling and alternative medical aesthetic purposes. High diversity exists in commercial formulations, notably the optimization of finished product stability, functionality, performance. Polyvalent ingredients such as calcium hydroxylapatite (CaHA) or vitamin B3 (niacinamide) bio-stimulants to improve skin quality attributes at administration site. The aim present study was perform multi-parametric characterization two novel...

Lyophilized organ extracts from livestock such as beef or elk are widely commercially proposed animal-sourced nutraceuticals. Such preparations contain blends of vitamins and nutrients that may be employed for general specific dietary supplementation. The first objective this study was to develop an optimized function-oriented manufacturing process stabilized organ-specific American buffalo (Bison bison). second the perform functional benchmarking obtained by focusing on antioxidant...

Background: Resilient hyaluronic acid (RHA) fillers are used to treat dynamic wrinkles or provide tissue lifting in facial aesthetics. This study explored the biological, biomechanical, and clinical safety performance of RHA4, a volumizing filler tailored for support areas, upon interaction with human subcutaneous adipose (AT). Methods: RHA4 underwent cytocompatibility testing fibroblasts stem cells. A 1-year rat vivo implantation tracked integration, local effects, degradation....

Skin aging is a complex and multifactorial process influenced by both intrinsic extrinsic factors. The periorbital area of the face particularly susceptible to premature signs due its delicate skin structure, major concern for many individuals. While hyaluronic acid (HA)-based dermal filler products are commonly used rejuvenation, novel approaches effectively locally address visible available. This study aimed investigate Innovyal Regenerative Action (IRA), an injectable polynucleotide-HA...

Abstract Incidence of allergic contact dermatitis (ACD) is rising worldwide as a consequence raising chemical exposure, especially from biocides present in common water-based products such cosmetics and household products. Understanding the biological processes involved skin sensitization crucial to mitigating ACD. Migration Langerhans cells basal lamina key step sensitization. Animal testing for has been largely replaced by alternative vitro methods due ethical concerns. This study presents...

Background: The extracellular matrix (ECM), primarily composed of collagen and elastin synthesized by dermal fibroblasts, is critical for mesenchymal tissue integrity. Fibroblast phenotypes vary significantly with the anatomical location developmental stage. Fetal skin, particularly prior to 14 weeks gestation, exhibits a simplified structure compared adult characterized thin, loose single-layered epithelium. Objectives: This study aimed characterize functionally compare homogenous...

Current restrictions for human cell-based therapies have been related to technological limitations with regards cellular proliferation capacity, maintenance of differentiated phenotype primary cell culture, and transmission communicable diseases. We seen that cultured fetal cells from one organ donation could possibly meet the exigent stringent technical aspects development therapeutic products. develop a master bank (MCB) 50 homogenous ampoules 4-5 million each (skin) in short periods time...

Abstract: For tissue engineering, several cell types and tissues have been proposed as starting material. Allogenic skin products available for therapeutic usage are mostly developed with culture foreskin of young individuals. Fetal cells offer a valuable solution effective safe engineering wounds due to their rapid growth simple culture. By selecting families genes that reported be implicated in wound repair particularly scarless fetal healing including transforming factor‐beta (TGF‐β)...

Cultured primary progenitor tenocytes in lyophilized form were previously shown to possess intrinsic antioxidant properties and hyaluronan-based hydrogel viscosity-modulating effects vitro. The aim of this study was prepare functionally characterize several stabilized (lyophilized) cell-free tenocyte extracts for inclusion cytotherapy-inspired complex injectable preparations. Fractionation sterilization methods included specific biotechnological manufacturing workflows such extracts....

Safety, quality and regulatory-driven iterative optimization of therapeutic cell source selection has constituted the core innovative developmental bedrock for primary fetal progenitor therapy in Switzerland throughout three decades. Customized Fetal Transplantation Programs were pragmatically devised as straightforward workflows tissue procurement, maximizing traceability, safety, consistency robustness progeny cellular materials. Whole-cell bioprocessing standardization yielded plethoric...

Progenitor Biological Bandages (PBB) have been continuously applied clinically in the Lausanne Burn Center for over two decades. Vast translational experience and hindsight gathered, specifically cutaneous healing promotion of donor-site grafts second-degree pediatric burns. PBBs constitute combined Advanced Therapy Medicinal Products, containing viable cultured allogeneic fetal dermal progenitor fibroblasts. Such constructs may partly favor repair regeneration functional tissues by...