A5067832764- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Glycosylation and Glycoproteins Research
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Mosquito-borne diseases and control
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Viral Infections and Vectors
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- HIV Research and Treatment
- Hepatitis C virus research
- Bacteriophages and microbial interactions
- Blood groups and transfusion
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- Endoplasmic Reticulum Stress and Disease
- Viral Infectious Diseases and Gene Expression in Insects
- Immunodeficiency and Autoimmune Disorders
- Protein purification and stability
- Diabetes and associated disorders
- Peptidase Inhibition and Analysis
- Mast cells and histamine
International Centre for Genetic Engineering and Biotechnology
2014-2024
National Center for Genetic Engineering and Biotechnology
2001-2009
University of Chile
2004
Institut Curie
2002
Inserm
2002
University of Trieste
1997-1998
Ospedale di Cattinara
1998
New York University
1997
AREA Science Park
1997
Peking University
1997
Abstract We recently demonstrated that a human recombinant scFv, L19, reacting with the ED‐B domain of fibronectin, marker angiogenesis, selectively targets tumoral vasculature in vivo. Using variable regions we constructed and expressed “small immunoprotein” (SIP) complete IgG1 performed biodistribution studies tumor‐bearing mice to compare blood clearance rate, vivo stability performance tumor targeting 3 L19 formats [dimeric scFv (scFv) 2 , SIP IgG1]. The accumulation different antibody...
In rotavirus-infected cells, the non-structural proteins NSP5 and NSP2 localize in complexes called viroplasms, where replication assembly occur. Recently, we have demonstrated direct interaction of with NSP2, as a consequence that, up-regulation hyperphosphorylation. To investigate possible structural role for NSP2-NSP5 interaction, analysed cytoplasmic distribution two transfected cells by immunofluorescence using specific antibodies. Here report that can drive formation viroplasm-like...
Several authors have reported on the effectiveness of alpha-interferon (IFN-alpha) in treatment patients with mixed cryoglobulinemia. This prompted to investigate long term effects this drug clinical, hematologic, and virologic parameters a group 20 (13 women 7 men) affected by cryoglobulinemia.In all patients, bone marrow biopsy, phenotyping cells, polymerase chain reaction (PCR) immunoglobulin gene rearrangement peripheral blood lymphocytes were performed before therapy at end follow-up. A...
RNA viruses induce the formation of subcellular organelles that provide microenvironments conducive to their replication. Here we show replication factories rotaviruses represent protein‐RNA condensates are formed via liquid–liquid phase separation viroplasm‐forming proteins NSP5 and rotavirus chaperone NSP2. Upon mixing, these readily form at physiologically relevant low micromolar concentrations achieved in cytoplasm virus‐infected cells. Early infection stage could be reversibly dissolved...
// Moitza Principe 1,2,* , Patrizia Ceruti Neng-Yao Shih 3,* Michelle S. Chattaragada 1,2 Simona Rolla Laura Conti 2,4 Marco Bestagno 5 Lorena Zentilin Sheng-Hui Yang 6 Paola Migliorini 7 Cappello Oscar Burrone and Francesco Novelli 1 Center for Experimental Research Medical Studies (CeRMS), Azienda Universitaria Ospedaliera Città della Salute e Scienza di Torino, Turin, Italy 2 Department of Molecular Biotechnology Health Sciences, University 3 National Institute Cancer Research,...
Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies have reported genetic diversity in antibody heavy chain and light genes found within individual hybridomas. As the prevalence of such has never been explored, we analyzed 185 random hybridomas, a large multicenter dataset. The hybridomas were not biased towards those with cloning difficulties or known additional chains. Of evaluated, 126 (68.1%) contained no productive chains, while remaining 59 (31.9%) one...
Summary. Clonal expansions of IgM‐producing B cells were investigated in 38 patients with a chronic hepatitis C virus infection. Eight affected type II mixed cryoglobulinaemia (two whom also had non‐Hodgkin's lymphoma and one Waldenstrom's disease), III cryoglobulinaemia, disease, 28 liver disease. To detect the clonal B‐cell we used RT/PCR procedure which CDR3/FW4 regions IgM heavy chain mRNAs amplified resolved sequencing poly‐acrylamide gels. Ig gene rearrangements detected all at high...
Viroplasms are discrete structures formed in the cytoplasm of rotavirus-infected cells and constitute replication machinery virus. The non-structural proteins NSP2 NSP5 localize viroplasms together with other viral proteins, including polymerase VP1, VP3 main inner-core protein, VP2. interact each other, activating hyperphosphorylation formation viroplasm-like (VLSs). We have used fused to enhanced green fluorescent protein (EGFP) investigate localization both within virus-infected cells, as...
ABSTRACT Rotaviruses, nonenveloped viruses presenting a distinctive triple-layered particle architecture enclosing segmented double-stranded RNA genome, exhibit unique morphogenetic pathway requiring the formation of cytoplasmic inclusion bodies called viroplasms in process involving nonstructural viral proteins NSP5 and NSP2. In these structures concerted packaging replication 11 positive-polarity single-stranded RNAs take place to generate (dsRNA) genomic segments. Rotavirus infection is...
Rotavirus viroplasms are cytosolic, electron-dense inclusions corresponding to the viral machinery of replication responsible for template transcription, dsRNA genome segments and assembly new cores. We have previously observed that, over time, those increase in size decrease number. Therefore, we hypothesized that this process was dependent on cellular microtubular network its associated dynamic components. Here, present evidence demonstrating structures, which, course an ongoing infection,...
Rotavirus (RV) replication takes place in the viroplasms, cytosolic inclusions that allow synthesis of virus genome segments and their encapsidation core shell, followed by addition second layer virion. The viroplasms are composed several viral proteins, including NSP5, which serves as main building block. Microtubules, lipid droplets, miRNA-7 among host components recruited viroplasms. We investigated interaction between RV proteins performing a pull-down assay lysates from RV-infected...
Rotavirus genomes contain 11 double-stranded (ds) RNA segments. Genome segment encodes the non-structural protein NSP5 and, in some strains, also NSP6. is produced soon after viral infection and localizes cytoplasmic viroplasms, where virus replication takes place. interference by small interfering (si) RNAs targeted to genome mRNA of two different strains blocked production a strain-specific manner, with strong effect on overall replicative cycle: inhibition viroplasm formation, decreased...
The immune synapse (IS) forms as dendritic cells (DCs) and T interact in lymph nodes during initiation of adaptive immunity. Factors that contribute to the formation maintenance IS stability function have been mostly studied cells, whereas little is known about events occurring DCs. Here, we show DCs activated by Toll-like receptor (TLR) agonists reorient microtubule-organizing center (MTOC) toward interacting cell antigen-specific through a mechanism depends on Rho GTPase Cdc42. IL-12,...
Abstract The Wiskott-Aldrich syndrome protein (WASp) is a key regulator of actin polimerization in hematopoietic cells. Mutations WASp cause severe immunodeficiency characterized by defective initiation primary immune response and autoimmunity. contribution altered dendritic cells (DCs) functions to the disease pathogenesis has not been fully elucidated. In this study, we show that conventional DCs develop normally WASp-deficient mice. However, Ag targeting lymphoid organ-resident via...
Rotavirus genome replication and the first steps of virus morphogenesis take place in cytoplasmic viral factories, called viroplasms, containing four structural (VP1, VP2, VP3 VP6) two non-structural (NSP2 NSP5) proteins. NSP2 NSP5 have been shown to be essential for viroplasm formation and, when co-expressed uninfected cells, form viroplasm-like structures (VLS). In present work, VLS was upon co-expression with core protein VP2 despite absence NSP2, indicating a central role assembly. Since...
Dengue virus (DENV) infection is a major emerging disease widely distributed throughout the tropical and subtropical regions of world affecting several millions people. Despite constants efforts, no specific treatment or effective vaccine yet available. Here we show novel design DNA immunisation strategy that resulted in induction strong antibody responses with high neutralisation titres mice against all four viral serotypes. The immunogenic molecule an engineered version domain III (DIII) E...
The rotavirus (RV) double-stranded RNA genome is replicated and packaged into virus progeny in cytoplasmic structures termed viroplasms. nonstructural protein NSP5, which undergoes a complex hyperphosphorylation process during RV infection, required for the formation of these virus-induced organelles. However, its roles viroplasm replication have never been directly assessed due to lack fully tractable reverse-genetics (RG) system rotaviruses. Here, we show novel application recently...
We have previously shown that a number of isoforms the non-structural rotavirus protein NSP5 are found in virus-infected cells. These differ their level phosphorylation which, at least part, appears to occur through autophosphorylation. co-localizes with another protein, NSP2, viroplasms infected cells where virus replication takes place. now show can be chemically cross-linked living viral polymerase VP1 and NSP2. Interaction NSP2 was also demonstrated by co-immunoprecipitation from...