A5047383675- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Neuroscience and Neuropharmacology Research
- Treatment of Major Depression
- Nerve injury and regeneration
- Neuroscience and Neural Engineering
- Genetics and Neurodevelopmental Disorders
- Cytokine Signaling Pathways and Interactions
- Genetic Neurodegenerative Diseases
- Receptor Mechanisms and Signaling
- Mental Health and Psychiatry
- RNA modifications and cancer
- Neurotransmitter Receptor Influence on Behavior
- Schizophrenia research and treatment
- RNA regulation and disease
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
University of Trento
2015-2024
Karolinska Institutet
2022
University of Helsinki
2022
Universität Innsbruck
2022
Mario Negri Institute for Pharmacological Research
2022
University of Pisa
1993-2018
University of Milan
2006-2016
UConn Health
2006
University of Edinburgh
2003-2005
RELX Group (United Kingdom)
2005
Huntingtin is a 350-kilodalton protein of unknown function that mutated in Huntington's disease (HD), neurodegenerative disorder. The mutant presumed to acquire toxic gain detrimental striatal neurons the brain. However, loss beneficial activity wild-type huntingtin may also cause death neurons. Here we demonstrate up-regulates transcription brain-derived neurotrophic factor (BDNF), pro-survival produced by cortical necessary for survival We show this lost when becomes mutated, resulting...
Pluripotent mouse embryonic stem (ES) cells multiply in simple monoculture by symmetrical divisions. In vivo, however, are generally thought to depend on specialised cellular microenvironments and undergo predominantly asymmetric Ex vivo expansion of pure populations tissue has proven elusive. Neural progenitor propagated combination with differentiating progeny floating clusters called neurospheres. The proportion neurospheres is low, they cannot be directly observed or interrogated. Here...
Expansion of a polyglutamine sequence in the N terminus huntingtin is gain-of-function event that causes Huntington's disease. This mutation affects primarily medium-size spiny neurons striatum. Huntingtin expressed many neuronal and non-neuronal cell types, implying more general function for wild-type protein. Here we report acts by protecting CNS cells from variety apoptotic stimuli, including serum withdrawal, death receptors, pro-apoptotic Bcl-2 homologs. protection may take place at...
Stable in vitro propagation of central nervous system (CNS) stem cells would offer expanded opportunities to dissect basic molecular, cellular, and developmental processes model neurodegenerative disease. CNS could also provide a source material for drug discovery assays cell replacement therapies. We have recently reported the generation adherent, symmetrically expandable, neural (NS) lines derived both from mouse human embryonic fetal forebrain (Conti L, Pollard SM, Gorba T, Reitano E,...
We report the construction of artificial cells that chemically communicate with mammalian under physiological conditions. The respond to presence a small molecule in environment by synthesizing and releasing potent protein signal, brain-derived neurotrophic factor. Genetically controlled engineered human embryonic kidney murine neural stem cells. data suggest are versatile chassis for situ synthesis on-demand release chemical signals elicit desired phenotypic changes eukaryotic cells,...
Abstract In vivo application of the CRISPR-Cas9 technology is still limited by unwanted Cas9 genomic cleavages. Long-term expression increases number loci non-specifically cleaved nuclease. Here we develop a Self-Limiting circuit for Enhanced Safety and specificity (SLiCES) which consists an unit Streptococcus pyogenes (SpCas9), self-targeting sgRNA second targeting chosen locus. The self-limiting results in increased genome editing controlling levels. For its utilization, next integrate...
Abstract Pediatric and adult high-grade gliomas are the most common primary malignant brain tumors, with poor prognosis due to recurrence tumor infiltration after therapy. Quiescent cells have been implicated in treatment resistance, but their direct visualization targeting remain challenging, precluding mechanistic study. Here, we identify a population of expressing Prominin-1 non-proliferating state pediatric glioma patients. Using genetic tool visualize ablate quiescent mouse cancer human...
Neural progenitor cells have been isolated from the embryonic central nervous system (CNS) of several mammalian species. These exhibit properties immature cells, including expression intermediate filament protein Nestin, ability to self renew, and give rise terminally differentiated cell types. In this study we describe some ST14A which were established via retroviral transduction temperature-sensitive mutant SV40 Large T Antigen into primary derived day 14 (E14) rat Striatum primordia. At...
The Repressor Element 1 Silencing Transcription factor (REST/NRSF) is a master repressor of neuronal programs in non-neuronal lineages shown to function as central regulator developmental and stem cell physiology. Aberrant REST has been associated with number pathological conditions. In cancer biology, play tumor suppressor activity epithelial cancers but an oncogenic role brain childhood malignancies such neuroblastoma medulloblastoma. Here we examined expression human glioblastoma...
The presence and activation of members the Janus Kinases/Signal Transducers Activator Transcription proteins in response to specific cytokines is currently focus intense investigation hematopoietic system. Although some evidence suggests that might play an important role brain development pathologies, very limited information available on JAK/STAT Central Nervous System. Here we provide Western blot immunohistochemistry data Jak2 vivo immature brain, its expression being greater early stages...
A correlation was found among the degree of memory loss, intellectual impairment, quantity senile plaques, and a decrease in choline acetyltransferase acetylcholinesterase activity patients affected by dementia Alzheimer type. In this study, were subjected to series computerized electroencephalographic (EEG) recordings neuropsychological evaluations after acute administration following drugs: placebo; cholinergic drugs physostigmine. pyridostigmine bromide, edrophonium chloride;...
The RNA-binding protein HuD promotes neurogenesis and favors recovery from peripheral axon injury. interacts with many mRNAs, altering both stability translation efficiency. We generated a nucleotide resolution map of the RNA interactome in motor neuron-like cells, identifying target sites 1,304 almost exclusively 3′ UTR. binds mRNAs encoding mTORC1-responsive ribosomal proteins factors. Altered expression correlates efficiency these overall synthesis, mTORC1-independent fashion. predominant...