A5056583671- Zebrafish Biomedical Research Applications
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Histone Deacetylase Inhibitors Research
- Multiple Myeloma Research and Treatments
- Aquaculture disease management and microbiota
- Acute Myeloid Leukemia Research
- Muscle Physiology and Disorders
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Advanced Fluorescence Microscopy Techniques
- Developmental Biology and Gene Regulation
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Research and Splicing
- Vibrio bacteria research studies
- Optical Coherence Tomography Applications
- Neonatal Respiratory Health Research
- Cystic Fibrosis Research Advances
- Genetics and Neurodevelopmental Disorders
- Peptidase Inhibition and Analysis
- Axon Guidance and Neuronal Signaling
- Hedgehog Signaling Pathway Studies
University of Milan
2014-2025
Politecnico di Milano
2019
Vita-Salute San Raffaele University
2012
KU Leuven
2010
Erasmus MC
2010
University Medical Center Utrecht
2010
Hubrecht Institute for Developmental Biology and Stem Cell Research
2010
University of Massachusetts Chan Medical School
2010
Max Planck Institute for Molecular Biomedicine
2010
Abstract Cystic fibrosis (CF) is a hereditary disease due to mutations in the CFTR gene and causes mortality humans mainly respiratory infections caused by Pseudomonas aeruginosa . In previous work we used phage therapy, which treatment with mix of phages, actively counteract acute P mice Galleria mellonella larvae. this apply therapy PAO1 CF zebrafish model. The structure channel evolutionary conserved between fish mammals cftr -loss-of-function embryos show phenotype that recapitulates...
In the last years, extracellular vesicles (EVs) from different plant matrices have been isolated and gained interest of scientific community for their intriguing biological properties. this study, we characterized nanovesicles lemon juice (LNVs) evaluated antioxidant effects. We tested LNV activity using human dermal fibroblasts that were pre-treated with LNVs 24 h then stimulated hydrogen peroxide (H
To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development.In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b Notch-6 in impaired lymphangiogenesis. Dll4/Notch silencing reduced number producing string parchordal lymphangioblasts; instead, connecting intersomitic vessels were formed. At a later...
Histone deacetylase 8 (HDAC8), a class I HDAC that modifies non-histone proteins such as p53, is highly expressed in different haematological neoplasms including subtype of acute myeloid leukaemia (AML) bearing inv(16). To investigate HDAC8 contribution to haematopoietic stem cell maintenance and leukaemic transformation, we generated zebrafish model with Hdac8 overexpression observed an increase stem/progenitor cells, phenotype could be reverted using specific inhibitor, PCI-34051 (PCI). In...
Abstract Background The homeodomain transcription factor Orthopedia (Otp) is essential in restricting the fate of multiple classes secreting neurons neuroendocrine hypothalamus vertebrates. However, there little information on intercellular factors that regulate Otp expression during development. Results Here, we identified two otp orthologues zebrafish ( otp1 and otp2 ) explored context morphogenetic pathways specify neuroectodermal regions. During forebrain development, expressed anterior...
Cornelia de Lange Syndrome is a severe genetic disorder characterized by malformations affecting multiple systems, with common feature of mental retardation. Genetic variants within four genes (NIPBL (Nipped-B-like), SMC1A, SMC3, and HDAC8) are believed to be responsible for the majority cases; all these encode proteins that part 'cohesin complex'. Cohesins exhibit two temporally separated major roles in cells: one controlling cell cycle other involved regulating gene expression. The present...
Can high resolution array-CGH analysis on a cohort of women showing primary ovarian insufficiency (POI) phenotype in young age identify copy number variants (CNVs) with deleterious effect function?This approach has proved effective to clarify the role CNVs POI pathogenesis and better unveil both novel candidate genes pathogenic mechanisms.POI describes progression toward cessation function before 40 years. Genetic causes are highly heterogeneous despite several being associated failure, most...
Multiple myeloma (MM) is an incurable hematologic neoplasm, whose poor prognosis deeply affected by the propensity of tumor cells to localize in bone marrow (BM) and induce protumorigenic activity normal BM cells, leading events associated with progression, including angiogenesis, osteoclastogenesis, spread osteolytic lesions. The interplay between MM niche does not only rely on direct cell-cell interaction, but a crucial role also played MM-derived extracellular vesicles (MM-EV). Here, we...
Abstract Citron Kinase (CITK) is a protein encoded by the CIT gene, whose pathogenic variants underlie microcephalic phenotypes that characterize MCPH17 syndrome. In neural progenitors, CITK loss leads to microtubule instability, resulting in mitotic spindle positioning defects, cytokinesis failure, and accumulation of DNA double strand breaks (DSBs), ultimately TP53-dependent senescence apoptosis. Although damage has been associated with impaired homologous recombination (HR), role this...
Abstract The Netrin-1 receptor UNC5B is an axon guidance regulator that also expressed in endothelial cells (ECs), where it finely controls developmental and tumor angiogenesis. In the absence of Netrin-1, induces apoptosis blocked upon binding. Here, we identify splicing isoform (called UNC5B-Δ8) exclusively by ECs generated through exon skipping NOVA2, alternative factor regulating vascular development. We show UNC5B-Δ8 a constitutively pro-apoptotic insensitive to required for specific...
Optical imaging through biological samples is compromised by tissue scattering and currently various approaches aim to overcome this limitation. In paper we demonstrate that an all optical technique, based on non-linear upconversion of infrared ultrashort laser pulses multiple view acquisition, allows the reduction effects in tomographic imaging. This namely Time-Gated Projection Tomography (TGOPT), used reconstruct three dimensionally internal structure adult zebrafish without staining or...
Vascular smooth muscle cells (VSMCs) are key participants in both early- and late-stage atherosclerosis influence neighbouring possibly by means of bioactive molecules, some which packed into extracellular vesicles (EVs). Proprotein convertase subtilisin/kexin type 9 (PCSK9) is expressed secreted VSMCs. This study aimed to unravel the role PCSK9 on VSMCs-derived EVs terms content functionality. were isolated from human VSMCs overexpressing (VSMCPCSK9-EVs) tested endothelial cells, monocytes,...
Abstract Background Prox1 , the vertebrate homolog of prospero in Drosophila melanogaster is a divergent homeogene that regulates cell proliferation, fate determination and differentiation during embryonic development. Results Here we report that, zebrafish, prox1 widely expressed several districts Central Nervous System (CNS). Specifically, evidenced expression group neurons, already positive for otp1 located hypothalamus at level posterior tuberculum (PT). knock-down determines severe loss...
Muscle regulatory factors activate myogenesis in all vertebrates, but their role has been studied great detail only the mouse embryo, where myogenin – Myod, Myf5 and Mrf4 are sufficient to (albeit not completely) skeletal myogenesis. In zebrafish myod myf5 required for induction of because simultaneous ablation prevents muscle development. Here we show that mrf4 myog can fully rescue myod/myf5 double morphant via a selective robust activation myod, keeping with its chromatin-remodelling...
Cornelia de Lange syndrome (CdLS), which is reported to affect ∼1 in 10 000 30 newborns, a multisystem organ developmental disorder with relatively mild severe effects. Among others, intellectual disability represents an important feature of this condition. CdLS can result from mutations at least five genes: nipped-B-like protein, structural maintenance chromosomes 1A, 3, RAD21 cohesin complex component and histone deacetylase 8 (HDAC8). It believed that these genes cause by impairing the...
The nucleophosmin 1 gene (NPM1) is the most frequently mutated in acute myeloid leukemia. Notably, NPM1 mutations are always accompanied by additional such as those cohesin genes RAD21, SMC1A, SMC3, and STAG2 but not regulator, nipped B-like (NIPBL). In this work, we analyzed a cohort of adult patients with leukemia mutation observed specific reduction expression NIPBL other genes. our zebrafish model, overexpression form also induced downregulation nipblb, ortholog human NIPBL. To...
Multiple myeloma is still incurable due to an intrinsic aggressiveness or, more frequently, the interactions of malignant plasma cells with bone marrow (BM) microenvironment. Myeloma educate BM support neoplastic cell growth, survival, acquisition drug resistance resulting in disease relapse. microenvironment characterized by Notch signaling hyperactivation increased expression Notch1 and 2 ligands Jagged1 tumor cells. activation influences biology promotes reprogramming stromal In this work...
Multi drug resistant (MDR) bacteria are insensitive to the most common antibiotics currently in use. The spread of antibiotic-resistant bacteria, if not contained, will represent main cause death for humanity 2050. situation is even more worrying when considering patients with chronic bacterial infections, such as those Cystic Fibrosis (CF). development alternative approaches essential and novel therapies that combine exogenous host-mediated antimicrobial action promising. In this work, we...