A5003717399- Effects and risks of endocrine disrupting chemicals
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Estrogen and related hormone effects
- Osteoarthritis Treatment and Mechanisms
- Bone Metabolism and Diseases
- Microplastics and Plastic Pollution
- Regulation of Appetite and Obesity
- Toxic Organic Pollutants Impact
- Nanoparticles: synthesis and applications
- Adipokines, Inflammation, and Metabolic Diseases
- Periodontal Regeneration and Treatments
- Sexual Differentiation and Disorders
- Hypothalamic control of reproductive hormones
- Pharmacological Effects of Medicinal Plants
- Carcinogens and Genotoxicity Assessment
- Hormonal and reproductive studies
- Prostate Cancer Treatment and Research
- Bone health and treatments
- Dental Trauma and Treatments
- Multiple Myeloma Research and Treatments
- Congenital limb and hand anomalies
- Antioxidant Activity and Oxidative Stress
- Extracellular vesicles in disease
- Melanoma and MAPK Pathways
University of Milan
2016-2025
Istituto Nazionale di Fisica Nucleare, Sezione di Milano
2013-2014
The presence and potential toxicity of nanoplastics (NPs) in aquatic ecosystems is an issue growing concern. Although many studies have investigated the adverse effects short-term exposure to high concentrations NPs organisms, information on consequences caused by administration low over long-term limited. present study aimed at investigating induced a (21-days) two sub-lethal polystyrene (PS-NPs; 0.05 0.5 µg/mL) Daphnia magna. A multi-level approach was performed assess sub-individual...
Abstract Malignant melanoma is the leading cause of death from skin cancer. Drug toxicity and resistance represent a serious challange for treatments. Evidence demonstrates that natural compounds may play crucial role in cancer prevention, growth progression. Vitamin E tocotrienols (TT) were shown to possess antitumor activity. Here, we analyzed effects δ-TT on cell involvement endoplasmic reticulum (ER) stress this The experiments performed human lines, BLM A375. exerted significant...
Platelet-rich plasma (PRP) is a platelet concentrate in small volume of plasma. It highly enriched growth factors able to stimulate the migration and bone-forming cells. PRP often used clinical applications, as dental surgery fracture healing. Platelet derived factor (PDGF), concentrated it was shown our previous studies provide chemotactic stimulus SaOS-2 osteoblasts move microchemotaxis assay. Aim present analyze effects pretreatment (short time course: 30-150 min) cells with on...
The exposure to environmental endocrine disrupting compounds (EDC), as polychlorinated biphenyls (PCBs), widely diffused in the environment may produce epigenetic changes that affect system. We found PCBs activate AR transcriptional activity and this effect is potentiated by demethylase Jarid1b, a histone catalyzes removal of trimethylation lysine 4 on H3 (H3K4me3), induced PCB. aim present study was investigate treatment cultured cells (HEK293) with mixture most and, also...
Background Cutaneous melanoma is an aggressive malignancy; its incidence increasing worldwide and prognosis remains poor. Clinical observations indicate that estrogen receptor β (ERβ) expressed in tissues expression decreases with tumor progression, suggesting suppressive function. These experiments were performed to investigate the effects of ERβ activation on cell growth. Methods Results Protein was analyzed by Western blot immunofluorescence assays. Cell proliferation assessed counting...
Multiple myeloma (MM) is an incurable hematologic neoplasm, whose poor prognosis deeply affected by the propensity of tumor cells to localize in bone marrow (BM) and induce protumorigenic activity normal BM cells, leading events associated with progression, including angiogenesis, osteoclastogenesis, spread osteolytic lesions. The interplay between MM niche does not only rely on direct cell-cell interaction, but a crucial role also played MM-derived extracellular vesicles (MM-EV). Here, we...
Our world is made of plastic. Plastic waste deeply affects our health entering the food chain. The degradation and/or fragmentation plastics due to weathering processes result in generation nanoplastics (NPs). Only a few studies tested NPs effects on human health. toxic actions are, part, mediated by oxidative stress (OS) that, among its effects, bone remodeling. This study aimed assess if influence skeleton remodeling through OS. Murine cell cultures (MC3T3-E1 preosteoblasts, MLOY-4...
PDGF is a major constituent of platelet rich plasma (PRP), responsible chemotactic and possibly mitogenic effects PRP on osteoblasts. family includes 5 isoforms: PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC PDGF-DD, all expressed in platelets except PDGF-DD. Aim this study was to analyze the effect recombinant hPDGF-A, -AB, -B -C, migration proliferation human osteoblastic cell line, SaOS-2. Preliminary observations were also done primary cultures In vitro microchemotaxis 3H-thymidine assays used....
Background: The epigenome represents an important target of environmental pollution. Early-life exposure to polychlorinated biphenyls (PCBs) modifies sex steroid enzymes and receptor transcription patterns. Steroid receptors, such as androgen (AR), function coregulators histone modification enzymes. Aim: To clarify if a PCB early-life might affect the in rat liver, we analyzed some post-translational modifications (H3K4me3 H4K16Ac) corresponding remodeling enzymes, AR enzyme coregulator....
Natural antioxidants are considered as promising compounds in the prevention/treatment of osteoporosis. We studied ability purified δ-tocotrienol (δ-TT) isolated from a commercial palm oil (Elaeis guineensis) fraction to protect osteoblast MC3T3-E1 and osteocyte MLO-Y4 cells against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage mechanisms involved its protective action MC3T3-E1.MC3T3-E1 were treated with δ-TT (1.25-20 µg/ml for 2 h) followed by t-BHP at 250 µM or 125 3 h,...
Abstract The ‘fate’ of growth plate hypertrophic chondrocytes has been long debated with two opposing theories: cell apoptosis or survival transformation into osteogenic cells. This study was carried out on the proximal tibial rabbits using light microscopy, scanning and transmission electron microscopy. We focused particularly orientation specimens included in order to define mineral deposition vascular invasion lines obtain histological ultrastructural images at corresponding height plate....