A5016221798- Regulation of Appetite and Obesity
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Biochemical Analysis and Sensing Techniques
- Growth Hormone and Insulin-like Growth Factors
- Acute Myocardial Infarction Research
- Bone health and osteoporosis research
- Historical and Literary Analyses
- Cardiac Imaging and Diagnostics
- Pancreatitis Pathology and Treatment
- Pain Mechanisms and Treatments
- Bone Metabolism and Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Helicobacter pylori-related gastroenterology studies
- Hypothalamic control of reproductive hormones
- Mast cells and histamine
- Historical and Literary Studies
- Stress Responses and Cortisol
- Biochemical effects in animals
- Psychoanalysis and Psychopathology Research
- Pituitary Gland Disorders and Treatments
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Italian Literature and Culture
- Amino Acid Enzymes and Metabolism
University of Milan
2012-2021
Italian Institute of Technology
2019-2021
Sapienza University of Rome
2019
Fondazione Poliambulanza Istituto Ospedaliero
2012
Istituto Dermopatico dell'Immacolata
2007
Clínica Santa María
2006
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
1991-2005
The Neurological Institute
1994
University of Pavia
1994
Istituto De Angeli (Italy)
1982-1989
### a. Background In 1999, the National Academy of Clinical Biochemistry (NACB)1 published first standards laboratory practice addressing analytical and clinical recommendations for use cardiac markers in coronary artery diseases (1). The objectives were to recommend appropriate implementation utilization biomarkers, specifically troponin (cTn), which had just gained US Food Drug Administration (FDA) clearance as a biomarker aid diagnosis acute myocardial infarction (AMI). In 2001, IFCC...
Ghrelin, the endogenous ligand for GH secretagogue receptors, has been reported to influence acid gastric secretion and motility, but its potential gastroprotective effect is unknown. The aims of this study were 1) examine effects central peripheral administration ghrelin on ethanol-induced ulcers in conscious rats, 2) investigate possible roles nitric oxide (NO), vagal nerve, sensory fibers ghrelin. Ghrelin was administered either intracerebroventricularly or sc 30 min before ethanol,...
We examined the possible central and peripheral effects of synthetic growth hormone secretagogues (GHS), hexarelin (Hexa) EP 40737 (D-Thr-D-Trp (2-Me)-Ala- Trp-D-Phe-Lys-NH2), their endogenous counterpart, ghrelin, on gastric acid secretion. The compounds were administered intracerebroventricularly (i.c.v.) or subcutaneously (s.c.) in conscious male rats volume secretion output 3 h after pylorus ligation (Shay-test). Central Hexa, ghrelin administration (from 0.1 pmol to 1 nmol/rat, i.c.v.)...
Background and purpose: Ghrelin, a gut‐brain peptide, is considered gastroprotective factor in gastric mucosa. We investigated the role of prostaglandins (PG) possible interplay between PGs nitric oxide (NO) ghrelin gastroprotection against ethanol (EtOH)‐induced lesions. Experimental approach: examined effects (1) central (4 μg per rat) injection on PGE 2 accumulation normal or EtOH–lesioned mucosa, (2) pretreatment with indomethacin (10 mg kg −1 , p.o.), non‐selective cyclooxygenase (COX)...
The effect of amylin on gastric ulcers induced by oral administration indomethacin (Indo, 20 mg kg −1 at a dosing volume 5 ml) or ethanol 50% (EtOH, 1 ml/rat) was investigated in conscious rats. Amylin given intracerebroventricularly (0.22, 0.66 and 2.2 μg/rat, i.c.v.) demonstrated dose‐dependent cytoprotective against both Indo EtOH‐induced ulcers. In contrast, amylin, subcutaneously doses effective inhibiting acid secretion (2.5, 10 40 μg , s.c), did not show any effect. interaction...
Natural antioxidants are considered as promising compounds in the prevention/treatment of osteoporosis. We studied ability purified δ-tocotrienol (δ-TT) isolated from a commercial palm oil (Elaeis guineensis) fraction to protect osteoblast MC3T3-E1 and osteocyte MLO-Y4 cells against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage mechanisms involved its protective action MC3T3-E1.MC3T3-E1 were treated with δ-TT (1.25-20 µg/ml for 2 h) followed by t-BHP at 250 µM or 125 3 h,...
Vgf gene expression has been detected in various endocrine and neuronal cells the gastrointestinal tract. In this study we investigated pharmacological activity of different VGF-derived peptides. Among these, TLQP-21, corresponding to 556-576 fragment protein was unique active peptide, its profile further studied.The effects TLQP-21 were examined vitro by smooth muscle contraction isolated preparations from rat tract and, vivo, assessing gastric emptying rats. Rat stomach tissues also...
Recent research suggests a role for ghrelin in the modulation of inflammatory disorders. However, type receptor (GHS-R) involved both anti-inflammatory and anti-hyperalgesic actions remains to be characterized. In this study, we examined whether inhibitory effect development hyperalgesia edema induced by intraplantar carrageenan administration depends on an interaction with GHS-R1a. Both central (1 nmol/rat, i.c.v.) peripheral (40 nmol/kg, i.p.) selective GHS-R1a agonist EP1572 had no...
Analytical, within-subject and between-subject components of variation have been determined for total alkaline phosphatase (ALP) its bone isoform, osteocalcin, tartrate-resistant acid (TR-ACP) in serum specimens from a cohort 10 healthy subjects over 1-month period. From these data, we calculated the desirable analytical imprecisions, indices individuality, critical differences significant change detection, number required to estimate homeostatic setpoint an individual. For ALP most observed...
Bone remodeling and repair require osteogenic cells to reach the sites that need be rebuilt, indicating stimulation of osteoblast migration could a promising osteoanabolic strategy. We showed purified δ-tocotrienol (δ-TT, 10 μg/mL), isolated from commercial palm oil (Elaeis guineensis) fraction, stimulates both MC3T3-E1 osteoblast-like primary human bone marrow mesenchymal stem (BMSC) as detected by wound healing assay or Boyden chamber respectively. The ability δ-TT promote is dependent on...
Ghrelin, the endogenous ligand of GH secretagogue receptor (GHS-R) has been previously shown to inhibit gastric acid secretion in pylorus-ligated rats. Two isoforms GHS-R have identified: GHS-R(1a) and GHS-R(1b). The present study aimed: (i) characterise type involved central inhibitory activity ghrelin by using des-octanoyl ghrelin, synthetic agonist (EP1572) antagonist (D-Lys(3)-GHRP-6) (ii) investigate relationship between cortistatin (CST) control natural neuropeptide CST-14 octapeptide...
We assessed the analytical and biological variation of pyridinium crosslinks in early morning, 2 h fasting, 24 urine specimens from 14 healthy adults over a 1 month period. The results were expressed both terms pyridinoline concentration pyridinoline/creatinine ratio. data obtained used to select optimum specimen for clinical purposes. found that: ( a) morning are preferred; b) should be as ratio; c) reference intervals stratified according gender; d) necessary imprecision (CV≤ 9%), derived...
The effects of histamine and amodiaquine (a drug known to inhibit histamine-N-methyltransferase) on pulsatile growth hormone (GH) secretion were determined in unanaesthetized male rats. Intracerebroventricular (i.c.v.) administration (0.01, 0.05, 0.1 μmol/rat) caused a dose-related suppression spontaneous pulses rat GH (rGH) secretion. Amodiaquine (0.16 μmol/rat i.c.v.) also inhibited the pulses, but lesser degree. results, which show inhibitory both exogenous increased levels endogenous rGH...