A5010514068- Nerve injury and regeneration
- Telomeres, Telomerase, and Senescence
- Neuropeptides and Animal Physiology
- Regulation of Appetite and Obesity
- Cancer Research and Treatments
- Signaling Pathways in Disease
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Glioma Diagnosis and Treatment
- Cellular transport and secretion
- Silk-based biomaterials and applications
- Hypothalamic control of reproductive hormones
- Apelin-related biomedical research
- Adipose Tissue and Metabolism
- S100 Proteins and Annexins
- Axon Guidance and Neuronal Signaling
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Neuroendocrine regulation and behavior
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Biochemical Analysis and Sensing Techniques
- Monoclonal and Polyclonal Antibodies Research
- Neurogenesis and neuroplasticity mechanisms
Institute of Cell Biology and Neurobiology
2011-2024
National Research Council
1999-2017
Istituto di Farmacologia Traslazionale
2012
Istituti di Ricovero e Cura a Carattere Scientifico
2011
Fondazione Santa Lucia
2011
Institute of Neurobiology and Molecular Medicine
1997-2009
University of Rome Tor Vergata
1989-2004
Istituto Superiore di Sanità
2004
Institute of Marine Engineering
2004
Sapienza University of Rome
1999
The low-affinity p75 neurotrophin receptor is believed to participate with the Trk tyrosine kinase in formation of high-affinity binding sites for nerve growth factor (NGF). To investigate functional significance two NGF receptors, a truncated was stably expressed PC12 rat pheochromocytoma cells, yielding cells greatly reduced levels wild-type and normal levels. Although these were capable differentiation by NGF, very few detected. These findings indicate that may be functionally dissociated...
Nerve growth factor (NGF) is essential for the development and differentiation of sympathetic or sensory neurons. A complementary DNA was cloned that corresponds to a gene sequence induced more than 50-fold in cultured target cell line pheochromocytoma cells (PC12 cells) 5 hours after addition NGF. The messenger RNA encodes 90,000-dalton polypeptide may represent one primary events NGF-induced
The vgf gene has been identified as an energy homeostasis regulator. Vgf encodes a 617-aa precursor protein that is processed to yield incompletely characterized panel of neuropeptides. Until now, it was unproved assumption VGF-derived peptides could regulate metabolism. Here, VGF peptide designated TLQP-21 in rat brain extracts by means immunoprecipitation, microcapillary liquid chromatography-tandem MS, and database searching algorithms. Chronic intracerebroventricular (i.c.v.) injection...
The neurotrophin responsive gene vgf is widely expressed in central and peripheral neurones, certain neuroendocrine cell populations. Its encoded VGF precursor protein (proVGF1: 617 amino acids rat, 615 man, > 85% homology) gives rise to several low molecular weight species. We studied a range of neuronal cells, which VGF-processing products were prominent with an apparent 20 10 kDa (VGF20 VGF10, respectively). Such peptides recognized by antibodies specific for the C-terminal rat...
VGF is the designation for a new 712 amino acid protein, regulated by nerve growth factor (NGF) in PC12 cells, that has not been previously described CNS. Northern blot analysis with nick-translated cDNA probe revealed single band of mRNA brain molecular weight identical to found cells. The current paper presents series immunocytochemical studies expression focus on hypothalamus. Two different antisera were raised against nonoverlapping sequences bacterial-expressed protein from gene cloned...
The peptides encoded by the VGF gene are gaining biomedical interest and increasingly being scrutinized as biomarkers for human disease. An endocrine/neuromodulatory role has been suggested but never demonstrated. Furthermore, no study demonstrated so far existence of a receptor-mediated mechanism any peptide. In present study, we provide comprehensive in vitro, ex vivo identification novel pro-lipolytic pathway mediated TLQP-21 We show first time that VGF-immunoreactivity is within...
Caspase-8 is a key player in extrinsic apoptosis and its activity often downregulated cancer. However, human expression retained some tumors, including glioblastoma (GBM), suggesting that it may support cancer growth these contexts. GBM, the most aggressive of gliomas, characterized by extensive angiogenesis an inflammatory microenvironment development resistance to therapies. We have recently shown sustains neoplastic transformation vitro GBM cell lines. Here, we demonstrate Caspase-8,...
While the central role played by TrkA in nerve growth factor (NGF) signalling has been established dissecting its signal transduction pathways, insight into mechanism of action p75LNR, low-affinity neurotrophin receptor, only recently achieved. The relative contribution p75LNR and to constitution high-affinity receptors for NGF and, similarly, with TrkB an TrkC formation those other neurotrophins, is presently under debate. Some form collaboration mediating activities observed between Trk...
Defective expression of frataxin is responsible for the degenerative disease Friedreich's ataxia. Frataxin a protein required cell survival since complete knockout lethal. protects tumor cells against oxidative stress and apoptosis but also acts as suppressor. The molecular bases this apparent paradox are missing. We therefore sought to investigate pathways through which enhances resistance in cells. found that upregulated several lines response hypoxic stress, condition often associated...
Abstract The c-Myc protein is dysregulated in many human cancers and its function has not been fully elucitated yet. inhibitor Omomyc displays potent anticancer properties animal models. It perturbs the network, impairs binding to E-boxes, retaining transrepressive inducing histone deacetylation. Here we have employed further analyse activity at epigenetic level. We show that both Myc stimulate H4 symmetric dimethylation of arginine (R) 3 (H4R3me2s), glioblastoma HEK293T cells. Consistently,...
Abstract: VGF is a neuroendocrine‐specific gene product that up‐regulated by nerve growth factor in the PC12 cell line. In rat neuroendocrine tissues two polypeptides of 90 and 80 kDa were detected an antiserum to N‐terminal domain (from residues 4 240). parallel, directed against C‐terminal nonapeptide 609 617) revealed several additional posttranslational products. Peptides apparent molecular sizes 20, 18, 10 prominent hypophysis but absent adrenal medulla, their relative abundance varied...
Abstract Different VGF peptides derived from Vgf, originally identified as a nerve growth factor responsive gene, have been detected in neurons within the central and peripheral nervous system various endocrine cells. In current study, we evaluated ability of TLQP‐21, VGF‐derived peptide, to protect, dose‐ time‐dependent manner, primary cultures rat cerebellar granule cells (CGCs) serum potassium deprivation‐induced cell death. We demonstrated that TLQP‐21 increased survival CGCs by...
Reaction of the thiol reagent 5,5′‐dithio‐bis(2‐nitrobenzoic acid) (Nbs 2 ) with brain‐specific protein S‐100 favours stabilization quaternary structure via disulfide bond formation. This process is modulated by those cations (Ca 2+ and K + which are known to affect conformation protein. Ca markedly reaction Nbs but inhibits subsequent formation; , on contrary, much less effective in promoting interaction strongly stimulates These findings interpreted assuming that presence three subunits...