A5026741989- Regulation of Appetite and Obesity
- Growth Hormone and Insulin-like Growth Factors
- Biochemical Analysis and Sensing Techniques
- Neuropeptides and Animal Physiology
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Knee injuries and reconstruction techniques
- Pancreatic function and diabetes
- Pituitary Gland Disorders and Treatments
- Hypothalamic control of reproductive hormones
- Tendon Structure and Treatment
- Receptor Mechanisms and Signaling
- Muscle Physiology and Disorders
- Osteoarthritis Treatment and Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Estrogen and related hormone effects
- Neuroscience and Neuropharmacology Research
- Diet, Metabolism, and Disease
- Axon Guidance and Neuronal Signaling
- Thyroid Disorders and Treatments
- Nerve injury and regeneration
- Ion Channels and Receptors
- Amyotrophic Lateral Sclerosis Research
- Metabolism, Diabetes, and Cancer
- Neurobiology and Insect Physiology Research
University of Milano-Bicocca
2015-2024
Electroformed Nickel (United States)
2022
Institut des Biomolécules Max Mousseron
2007-2008
Centre National de la Recherche Scientifique
2007-2008
Université de Montpellier
2003-2007
University of Parma
2003-2005
University of Milan
1988-2003
University of Modena and Reggio Emilia
2003
University of Brescia
2003
University of Virginia
1996
Anterior cruciate ligament (ACL) reconstruction restores knee stability but does not reduce the incidence of posttraumatic osteoarthritis induced by inflammatory cytokines. The aim this research was to longitudinally measure IL-1 β , IL-6, IL-8, IL-10, and TNF- α levels in patients subjected ACL using bone-patellar tendon-bone graft. Synovial fluid collected within 24–72 hours rupture ( acute ), 1 month after injury immediately prior surgery presurgery thereafter postsurgery ). For...
The treatment of anterior cruciate ligament (ACL) injuries in children and adolescents is challenging. Preclinical clinical studies investigated ACL repairing techniques skeletally immature subjects. However, intra-articular bioenvironment following tear has not yet been defined patients. aim this study was to measure cytokine concentrations the synovial fluid adolescent population. Synovial levels IL-1β, IL-1ra, IL-6, IL-8, IL-10, TNF-α were measured 17 patients (15 boys) with tears who...
The critical role of neuroinflammation in favoring and accelerating the pathogenic process Alzheimer's disease (AD) increased need to target cerebral innate immune cells as a potential therapeutic strategy slow down progression. In this scenario, mesenchymal stem (MSCs) have risen considerable interest thanks their immunomodulatory properties, which been largely ascribed release extracellular vesicles (EVs), namely exosomes microvesicles. Indeed, beneficial effects MSC-EVs regulating...
Ghrelin, the endogenous ligand for GH secretagogue receptors, has been reported to influence acid gastric secretion and motility, but its potential gastroprotective effect is unknown. The aims of this study were 1) examine effects central peripheral administration ghrelin on ethanol-induced ulcers in conscious rats, 2) investigate possible roles nitric oxide (NO), vagal nerve, sensory fibers ghrelin. Ghrelin was administered either intracerebroventricularly or sc 30 min before ethanol,...
It has previously been reported that growth hormone secretagogues (GHS) may have a role in the regulation of bone metabolism animals and humans. In this study we evaluated effect ghrelin, endogenous ligand GHS receptors, on proliferation rate osteoblast activity primary cultures rat calvaria osteoblasts. same experiments, compared effects ghrelin with those hexarelin (HEXA) EP-40737, two synthetic different characteristics. Both HEXA (10 −11 −10 −8 M) significantly stimulated at low...
Abstract Surgical reconstruction of the anterior cruciate ligament (ACL) does not necessarily decrease risk developing osteoarthritis (OA). The inflammatory response and relative changes in pro‐ anti‐inflammatory cytokines could participate triggering development OA. To test this hypothesis we measured concentrations IL‐1β, IL‐1ra, IL‐6, IL‐8, IL‐10, TNF‐α at different times after ACL rupture. sample population consisted 48 patients with tear which were assigned to groups according time...
ABSTRACT Recently, data have been presented showing that muscarinic cholinergic agonists or antagonists can modulate, in opposite ways, GH-releasing hormone (GHRH)-induced GH release man. The aim of the present study was, first, to confirm these findings rat and, secondly, if confirmed, investigate mechanism(s) subserving effect drugs. In adult male rats bearing chronic indwelling atrial cannulae, pretreatment with pirenzepine (0·5 mg/kg, i.v.) atropine significantly reduced rise plasma...
The vgf gene has been identified as an energy homeostasis regulator. Vgf encodes a 617-aa precursor protein that is processed to yield incompletely characterized panel of neuropeptides. Until now, it was unproved assumption VGF-derived peptides could regulate metabolism. Here, VGF peptide designated TLQP-21 in rat brain extracts by means immunoprecipitation, microcapillary liquid chromatography-tandem MS, and database searching algorithms. Chronic intracerebroventricular (i.c.v.) injection...
We previously reported that induction of selective GH deficiency in the rat exacerbates cardiac dysfunction induced by experimental ischemia and reperfusion performed on explanted heart. In same model, short-term treatment with hexarelin, a GH-releasing peptide, reverted this effect, as did GH. To ascertain whether hexarelin had non-GH-mediated protective effects heart, we compared hypophysectomized rats. Hexarelin (80 μg/kg sc), given for 7 days, prevented exacerbation ischemia-reperfusion...
A series of ghrelin receptor ligands based on the trisubstituted 1,2,4-triazole structure were synthesized and evaluated for their in vitro binding biological activity. In this study, we explored significance aminoisobutyryl (Aib) moiety, a common feature numerous growth hormone secretagogues described literature. Potent agonist antagonist secretagogue type 1a (GHS-R1a) obtained, i.e., compounds 41 (JMV2894) 17 (JMV3031). The best vivo activity food intake, after sc injection rodents. Among...
A new series of growth hormone secretagogue (GHS) analogues based on the 1,2,4-triazole structure were synthesized and evaluated for their in vitro binding ability to stimulate intracellular calcium release cloned hGHS-1a ghrelin receptor expressed LLC PK-1 cells. We have potent ligands this receptor, some them behaving as agonists, partial or antagonists. Some compounds among most potent, i.e., agonist 29c (JMV2873), agonists including 21b (JMV2810), antagonists 19b (JMV2866) 19c (JMV2844),...
Data from Alzheimer's disease (AD) patients and AD animal models demonstrate the accumulation of inflammatory microglia at sites insoluble fibrillar beta-amyloid protein (fAbeta) deposition. It is known that fAbeta binds to CD36, a type B scavenger receptor also involved in internalization oxidized low-density lipoprotein (LDL), initiate signaling cascade regulates microglial recruitment, activation, secretion mediators leading neuronal dysfunction death. The recent demonstration binding...
The peptides encoded by the VGF gene are gaining biomedical interest and increasingly being scrutinized as biomarkers for human disease. An endocrine/neuromodulatory role has been suggested but never demonstrated. Furthermore, no study demonstrated so far existence of a receptor-mediated mechanism any peptide. In present study, we provide comprehensive in vitro, ex vivo identification novel pro-lipolytic pathway mediated TLQP-21 We show first time that VGF-immunoreactivity is within...
Abstract Background Cachexia is a wasting condition associated with cancer types and, at the same time, serious and dose‐limiting side effect of chemotherapy. Skeletal muscle loss one main characteristics cachexia that significantly contributes to functional impairment. Calcium‐dependent signaling pathways are believed play an important role in skeletal decline observed cachexia, but whether intracellular calcium homeostasis affected this situation remains uncertain. Growth hormone...
ABSTRACT Concentrations of pro‐ and anti‐inflammatory cytokines in synovial fluid samples collected from patients with chronic meniscal tears were investigated. An acute inflammatory response is generally reported 24–48 h after knee injury, but the largest body data available literature concerns anterior cruciate ligament injury very little information about balance soluble factors knees tears. Sixty‐nine (46 males 23 females) tear that occurred more than 3 months earlier enrolled. According...
Palmitoylethanolamide (PEA) is an endogenous lipid produced on demand by neurons and glial cells that displays neuroprotective properties. It well known inflammation neuronal damage are strictly related processes microglia play a pivotal role in their regulation. The aim of the present work was to assess whether PEA could exert its anti-inflammatory effects through modulation reactive phenotypes. In N9 microglial cells, pre-incubation with blunted increase M1 pro-inflammatory markers induced...