A5028449400- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Nerve injury and regeneration
- Stress Responses and Cortisol
- Cancer, Stress, Anesthesia, and Immune Response
- Knee injuries and reconstruction techniques
- Osteoarthritis Treatment and Mechanisms
- Tendon Structure and Treatment
- Cancer Treatment and Pharmacology
- Immune Response and Inflammation
- Hypothalamic control of reproductive hormones
- Tryptophan and brain disorders
- Botulinum Toxin and Related Neurological Disorders
- Pharmacological Receptor Mechanisms and Effects
- Inflammatory mediators and NSAID effects
- Dermatology and Skin Diseases
- Pharmacological Effects of Natural Compounds
- Receptor Mechanisms and Signaling
- Pluripotent Stem Cells Research
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Circadian rhythm and melatonin
- Estrogen and related hormone effects
- Neuroinflammation and Neurodegeneration Mechanisms
- Anesthesia and Pain Management
- Cannabis and Cannabinoid Research
University of Milan
2015-2024
Città di Castello Hospital
2021
Politecnico di Milano
2016
University of Pisa
2010
Park Terrace Care Center
2006
Anterior cruciate ligament (ACL) reconstruction restores knee stability but does not reduce the incidence of posttraumatic osteoarthritis induced by inflammatory cytokines. The aim this research was to longitudinally measure IL-1 β , IL-6, IL-8, IL-10, and TNF- α levels in patients subjected ACL using bone-patellar tendon-bone graft. Synovial fluid collected within 24–72 hours rupture ( acute ), 1 month after injury immediately prior surgery presurgery thereafter postsurgery ). For...
The treatment of anterior cruciate ligament (ACL) injuries in children and adolescents is challenging. Preclinical clinical studies investigated ACL repairing techniques skeletally immature subjects. However, intra-articular bioenvironment following tear has not yet been defined patients. aim this study was to measure cytokine concentrations the synovial fluid adolescent population. Synovial levels IL-1β, IL-1ra, IL-6, IL-8, IL-10, TNF-α were measured 17 patients (15 boys) with tears who...
Abstract Surgical reconstruction of the anterior cruciate ligament (ACL) does not necessarily decrease risk developing osteoarthritis (OA). The inflammatory response and relative changes in pro‐ anti‐inflammatory cytokines could participate triggering development OA. To test this hypothesis we measured concentrations IL‐1β, IL‐1ra, IL‐6, IL‐8, IL‐10, TNF‐α at different times after ACL rupture. sample population consisted 48 patients with tear which were assigned to groups according time...
Neuropathic pain consequent to peripheral injury is associated with local inflammation and overexpression of nitric oxide synthases (NOS) inflammatory cytokines in locally recruited macrophages, Schwann glial cells. We investigated the time course localization central (spinal cord thalamus) nervous system (nerve dorsal root ganglia), a mouse model mononeuropathy induced by sciatic nerve chronic constriction injury. ATP recognized as an endogenous mediator. Therefore we also evaluated role...
Abstract Painful neuropathy is one of the complications diabetes mellitus that adversely affects patients’quality life. Pharmacological treatments are not fully satisfactory, and novel approaches needed. In a preclinical mouse model effect both human mesenchymal stromal cells from adipose tissue (hASC) their conditioned medium (hASC-CM) was evaluated. Diabetes induced by streptozotocin. After neuropathic hypersensitivity established, mice were intravenously injected with either 1 × 10 6 hASC...
<h3>Importance</h3> Convalescent plasma (CP) has been generally unsuccessful in preventing worsening of respiratory failure or death hospitalized patients with COVID-19 pneumonia. <h3>Objective</h3> To evaluate the efficacy CP plus standard therapy (ST) vs ST alone <h3>Design, Setting, and Participants</h3> This prospective, open-label, randomized clinical trial enrolled (1:1 ratio) pneumonia to receive between July 15 December 8, 2020, at 27 sites Italy. Hospitalized adults a partial...
There is great interest in soy isoflavones as alternatives to endogenous estrogens not only hormonal pathologies, but also inflammatory, neurodegenerative diseases, and pain. We investigated the effect of isoflavone genistein on neuropathic Genistein binds estrogen receptors (ER) with higher affinity for ERbeta particularly expressed neuronal immune cells. Neuropathy was induced mice by means chronic sciatic nerve constriction, subcutaneous administration from third day after lesion reversed...
This review provides an overview of the immunological effects commonly used analgesic opioid drugs, focusing mainly on two aspects: mechanisms involved and potential clinical relevance. The immunomodulatory morphine have been characterized in animal human studies. Morphine decreases effectiveness both natural acquired immunity, interfering with intracellular pathways immune regulation, directly indirectly via activation central receptors. targets at basis opioid-induced immunomodulation...
Introduction: Impaired immune function during the perioperative period may be associated with worse short- and long-term outcomes. Morphine is considered a major contributor to modulation. Patients methods: We performed pilot study investigate postoperative by analyzing peripheral blood mononuclear cells' functionality cytokine production in 16 patients undergoing abdominal surgery. All were treated intravenous (i.v.) patient-controlled analgesia morphine continuous wound infusion...
Summary In experimental neuropathic pain, intravenous neural stem cells induce a rapid remission of hyperalgesia and allodynia, followed by improvement in nerve morphology. A nonphysiological repair the lesioned leading to formation neurinomas, altered conduction, spontaneous firing is considered main cause events underlying pain. It was investigated whether cell (NSCs) administration could lead physiological repair, thus reduction pain symptoms such as allodynia well-established model this...
1.--The small protein Bv8, isolated from the amphibian skin, belongs to a novel family of secreted proteins linked several biological effects. We describe expression Bv8/prokineticins and their receptors in mouse macrophages, characterize proinflammatory activities. 2.--The rodent analogue prokineticin-2, is expressed by as well its G-protein-coupled receptor prokineticin (PKR-1 PKR-2). PKR-1 more abundantly. 3.-- Bv8 induces potent chemotaxis macrophages at concentrations low 10(-12) M....
A sixth month phase II multicenter-pilot trial with a low dose of the opiate antagonist Naltrexone (LDN) has been carried out in 40 patients primary progressive multiple sclerosis (PPMS). The end points were safety and tolerability. Secondary outcomes efficacy on spasticity, pain, fatigue, depression, quality life. Clinical biochemical evaluations serially performed. Protein concentration β-endorphins (BE) mRNA levels allelic variants μ-opiod receptor gene (OPRM1) analyzed. Five dropouts two...
Over the last decade, it has been proved that mesenchymal stem cells (MSCs) elicit anti-inflammatory effects. MSCs from adipose tissue (hASCs) differentiate into of mesodermal lineage and transdifferentiate ectodermal-origin cells. Although there are various etiologies to chronic pain, one common feature is painful states associated with increased inflammation. We believe in hASCs as a therapeutic tool also pathologies involving neuroinflammation neuronal damage. have investigated effect...
ABSTRACT Concentrations of pro‐ and anti‐inflammatory cytokines in synovial fluid samples collected from patients with chronic meniscal tears were investigated. An acute inflammatory response is generally reported 24–48 h after knee injury, but the largest body data available literature concerns anterior cruciate ligament injury very little information about balance soluble factors knees tears. Sixty‐nine (46 males 23 females) tear that occurred more than 3 months earlier enrolled. According...
We examined the changes in cerebrospinal fluid (CSF) concentrations of prostaglandin E2 (PGE2) and tumor necrosis factor-alpha (TNF-alpha) after intraplantar administration complete Freund's adjuvant (CFA) rats. In addition, we investigated whether different analgesic drugs orally administered at antihyperalgesic doses were able to prevent PGE2 TNF-alpha spinal levels associated with hindpaw inflammation.The Randall-Selitto paw-withdrawal test was used measure inflammatory hyperalgesia....